EMAGE mouse gene expression spatial database

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EMAGE:11573

Glyr1 glyoxylate reductase 1 homolog (Arabidopsis) ( MGI:1921272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003637. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003637_01	euxassay_003637_02	euxassay_003637_03	euxassay_003637_04

euxassay_003637_05	euxassay_003637_06	euxassay_003637_07	euxassay_003637_08	euxassay_003637_09

euxassay_003637_10	euxassay_003637_11	euxassay_003637_12	euxassay_003637_13	euxassay_003637_14

euxassay_003637_15	euxassay_003637_16	euxassay_003637_17	euxassay_003637_18	euxassay_003637_19

euxassay_003637_20	euxassay_003637_21	euxassay_003637_22	euxassay_003637_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11573_wholemount_strong.wlz

11573_wholemount_moderate.wlz

11573_wholemount_weak.wlz

11573_wholemount_possible.wlz

11573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6631
Entity Detected:	Glyr1, glyoxylate reductase 1 homolog (Arabidopsis) ( MGI:1921272)
Sequence:	sense strand is shown >T6631 GATGGCGGCTGTGAGTCTGCGGCTCGGCGACTTGGTGTGGGGGAAACTGGGCCGGTATCCTCCCTGGCCA GGAAAGATTGTTAATCCACCCAAGGACTTAAAGAAACCACGTGGAAAGAAATGCTTCTTTGTGAAGTTTT TTGGAACAGAAGATCATGCCTGGATCAAAGTGGAACAGCTAAAGCCTTACCATGCTCACAAGGAGGAGAT GATAAAGATTAACAAGGGTAAACGGTTCCAGCAAGCTGTGGATGCTGTTGAAGAGTTCCTCAGGAGAGCC AAAGGGAAAGACCAGACATCATCCCACACTTCTGCTGATGACAAGAATCGGCGTAATTCCAGTGAGGAGA GAAGTAGGCCAAACTCAGGTGATGAGAAACGCAAGCTTAGCCTGTCTGAAGGGAAGGTGAAGAAGAACAT GGGAGAAGGAAAGAAGAGGGTGACTTCAGGCTCTGCAGACAGAGGCTCCAAATGCCTTAAAAGAGCCCAA GAGCAAAGTCCCCGGAAGCGGGGTCGGCCCCCAAAGGATGAGAAGGACCTCACCATCCCTGAGTCTAGCA CTGTAAAGGGGATGATGGCTGGACCGATGGCTGCATTTAAATGGCAGCCAACAGCGACCGAGCCAGTCAA AGATGCAGATCCTCATTTTCATCATTTTCTGTTGAGCCAAACAGAGAAGCCAGCTGTCTGTTACCAGGCA ATCACAAAGAAGTTGAAAATATGTGAAGAGGAAACTGGTTCCACCTCCATCCAGGCAGCAGACAGCACAG CTGTGAATGGCAGCATTACACCCACAGACAAAAAGATAGGATTTTTGGGCCTTGGCTTAATGGGAAGTGG CATCGTCTCCAACTTGCTAAAAATGGGTCACACAGTGACTGTCTGGAACCGGACTGCAGAGAAAGAGGGG GCCCGCCTAGGAAGAACCCCCGCTGAAGTCGTTTCAACTTGTGACATCACTTTTGCCTGTGTGTCGGATC CAAAGGCAGCCAAGGACCTGGTGCTGGGCCCCAGCGGTGTGCTGCAAGGAATCCGCCCCGGGAAGTGCTA TGTGGACATGTCAACGGTGGATGCGGATACAGTCACCGAGCTGGCCCAGGTGATTGTATCCAGAGGGGGA CGCTTTCTGGAAGCACCAGTCTCAGGGAATCAGCAACTGTCCAATGATGGGATGTTGGTGATCTTAGCAG CCGGAGACAGGGGCTTGTATGAGGACTGCAGCAGCTGCTTCCAGGCAATGGGAAAGACCTCCTTCTTTTT AGGTGAAGTTGGCAATGCAGCCAAGATGATGCTGATTGTGAACATGGTCCAAGGGAGCTTCATGGCCACC ATTGCTGAGGGGCTCACGCTGGCCCAAGTGACAGGCCAATCACAGCAGACACTCTTGGACATCCTTAATC AGGGACAATTGGCCAGCATCTTTCTGGACCAGAAGTGCCAAAATATCCTACAAGGAAACTTTAAACCTGA CTTCTACCTGAAATACATTCAGAAGGATCTCCGCCTGGCCATTGCATTGGGTGATGCAGTCAACCACCCC ACTCCCATGGCAGCTGCAGCCAATGAGGTATACAAAAGAGCCAAGGCACTGGACCAGTCTGACAATGATA TGTCTGCCGTGTACCGAGCCTACATACACTAAGCCTTGCCTGCCCATCTACCCTCCCATCTTCCCTCTGA CCTCCCCTCCCAGTGTGGGGTCAGGGTTCTGGGACTAGCTCTGGACCAGTCACCTGTCTCCATTTCCTTT TATACAGACTTTGAAACTTGCATCAGCACAGTACACAGCAGCACCCTGTCTCCCTGAGGCCCTGGCCAGG GATAAGAGGATCTATCTCTCCTGGTGGCTGTGAAGAAGCTCTTGAGCTGGGCCTTGATTGACCCTGGAGC AGGAGGTTGTGGATTCTCTCTGTTCCTCTGTGTTCTCTGTCTCTGTCACACACACACCCAGGATAGAAGC TACCCAGAAACTGCTGCCTGGCTGTTCTTGCAAGCTTAGCCCCCTTCTGGTCAAGAGACTGGAGGGACTA GAATTGACAGGGAGCACTGGAAGCTTTCTTTCAACTTCCCCAGGACAGAGACTGGCCCCCACTGGGCCCC TGAAAGGAGAGGCCCCTCAGCCATCATGAACCAACAGATTCCCTGAGGAGGCTCTGTGGATCACCAGCTG AGGAGGATTTTCCTGTAGACACTACCATTACACCGCAGCTCCCAGAGTGTCTCTGTGAACTGAAGAGAGA CCAGCATAGCCACATAGACCCCTCTTCAGGGGGAGCAGAGTCTACAGTCTTCTGTCCTGAGCCTGTTCCT CCCAGGAGGTTTCATTTGGATTTGCATTCCAGCCCAAGGGAAGGGGCCGTAAGGGCCTTGGGAGCAGTGA AGTGTATGGGCGGCTCCTTCCTGTTTCCGTTACGCCAGGCTCTTGAGCTGGGCTACCTGTTCAGCCTGCT CTACACAGGTCCCCTGCATGTCCCTCTTCCCCTTTTCCTTCCATGGAGATATTTCTGTCCCATCCTCTTT TGACCCAGCCCCAGCCTGATCAACACCAATAATAAGCATTTCCTAGATGAGCATTTCCTAGGCTGGGCTT TTGATGAGGAACCTTACTTCTGCCAGTGGTTCCAAGTTCTGGGTTTTTTGGGACTTGAAGCATTTCTCCT TGAAGAGGGGACCAGAGAGGCTTAGAATGTCACACGAGAGGAATCACCCGAGGAAGCCTTCCTCCTCATG AGTTTGGATTTTCAAAGCCACGATTTAACTTTTCCATCTGGGATAAGGATCTCAGGACATGGGCACGGGG CAGGGGATGCTTGTGGAGTTCAGTGCAATTGTCCATTCACTGGGATGCTGAACAGTTTCTGTCTGGACAC AGCCTGTCTTGGTCCTGGGGGCTGGGGGTGGGGAAGATGCTGTAAGAAAACCGGTTAGTCAATGTTGTCT TAATATTGTTGACAATTCTGTAAAGTTCCTTTTTATGACTATTTCTGTTTAAGCTATTTCACCTTTCATT TTGAAACCCTTTCCTTTTATGGAGAAAATGTGACACTGTGGAAATGCTTGTAAGAAAGCCCCTACTCCCG CTTTTCCTTGAAATGACAGATCTAGGTGATTACGGTTGTGAATTTTTATTTTCACTTTGTTTTTAATGAA CATTTGTCTTTCAGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599477 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599477 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11572 same embryo
	EMAGE:11574 same embryo
	EMAGE:11577 same embryo
	EMAGE:11576 same embryo
	EMAGE:11575 same embryo
	EurExpress:euxassay_003637 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source