EMAGE mouse gene expression spatial database

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EMAGE:11576

Nmnat3 nicotinamide nucleotide adenylyltransferase 3 ( MGI:1921330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003646. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003646_01	euxassay_003646_02	euxassay_003646_03	euxassay_003646_04

euxassay_003646_05	euxassay_003646_06	euxassay_003646_07	euxassay_003646_08	euxassay_003646_09

euxassay_003646_10	euxassay_003646_11	euxassay_003646_12	euxassay_003646_13	euxassay_003646_14

euxassay_003646_15	euxassay_003646_16	euxassay_003646_17	euxassay_003646_18	euxassay_003646_19

euxassay_003646_20	euxassay_003646_21	euxassay_003646_22	euxassay_003646_23	euxassay_003646_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11576_wholemount_strong.wlz

11576_wholemount_moderate.wlz

11576_wholemount_weak.wlz

11576_wholemount_possible.wlz

11576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6633
Entity Detected:	Nmnat3, nicotinamide nucleotide adenylyltransferase 3 ( MGI:1921330)
Sequence:	sense strand is shown >T6633 TGGGAGCGCAAGGTCAAGTTCACTCGCGGCTCCGCCCCCAGCCTGGGTCAAGACTAGTTAGTCTTAGGGC GATCTGCGGGGTGACCCATCCTGGTCGAGCAGCAGATCCGCGGTCCCTGTTGACTCTGAGCGGAGCCCCG CGACGCGCAGGTAAGCACAGCCAGCGACCTTGGCTGCCTGCTTCGCCCGCGTGGGTGTCCACGAAGCCTT GAGTCCCCGCGTACCTACCAGGCCCACGTGACTCTCGGGGGCGGCGACTCGGGCGCGCGCGCGCACGACA GAGTCAGATGGCTGTGGAGCGCAGAACCACGCTAGCCCCACGGTCACTTTTCTGACCGATTGGCTCGAGC TGACACGGGTTCGGATCCCCCCTGCAGCACGTTTACAGTCAGTTAAAACAGGGTGGCCACTGCGGGTGTG GAGGTGTGTGACAGCATGAAGAACCGAATCCCTGTGGTGCTTCTGGCCTGTGGTTCCTTCAACCCCATCA CGAATATGCACCTGCGCTTGTTTGAGGTGGCCAGAGACCACCTACACCAAACAGGAAGGTACCAGGTGAT TGAGGGCATCATCTCACCCGTCAATGACAGCTATGGGAAGAAAGACCTGGTGGCTTCCCATCACCGAGTG GCCATGGCCCGGCTGGCCCTGCAGACATCTGACTGGATTCGGGTGGACCCCTGGGAGAGTGAGCAGGCGC AGTGGATGGAAACGGTGAAGGTGCTGAGGCACCATCACAGGGAGCTGCTCAGATCCTCAGCCCAGATGGA TGGCCCAGACCCCAGCAAGACACCATCAGCCTCTGCAGCACTGCCAGAGTTGAAACTCCTCTGCGGAGCT GATGTCCTCAAGACCTTCCAGACCCCCAACCTCTGGAAAGACACGCACATCCAGGAAATAGTGGAGAAGT TCGGCTTGGTGTGCGTGAGCAGGAGCGGTCATGACCCGGAAAGGTACATCTCGGACTCGCCCATCCTCCA GCAGTTTCAGCACAACATTCACCTGGCCAGGGAACCCGTTCTGAACGAGATCAGTGCCACATACGTCAGG AAAGCCTTGGGACAAGGGCAGAGCGTGAAGTACCTCCTCCCTGAGGCCGTCATCACCTACATCAGGGACC AGGGCCTCTACATCAATGACGGTTCCTGGAAAGGGAAAGGAAAGACTGGCTAGAGGGCCTCCTGGGGAGT AGGGGTATTCTGCTCTGGGTTCTGGGTTTGTTTCCACAGTGGTTTTTTTTTACCATGGGTGACTTCCTAC TCCAGGAAGAGATCGTGCCTGTGGCAGAGACAGTCGAGTATCAGAGCAACTTAAGAGGCATGACAGGTCA TAAGATTAGGGTCACTAAGGAGGAGACCTGCTTACTGTGTAAATGGCAGTCATGGGAGGCACACTGATGC TGTCCTGAGCATGGTTGGCCCCTGAGGGGTCCATCAAGTCGCCTTCGTTCGGGTTTTGTTTTTGTTTTTT GTTTTATAACTCGGGCCAGGTGCAGCATGCTGGTCTTGATAGGCAGAGTTTGACAGGGCGCTAATTACTG AGCAGAGGACCTTTTCAACATCCTCGGCTCAAACAATAATAGCAAGTGAGGATTCAGAAATCTGGAAACA GAACATCATTGATTTCCAATGTATCCTCCCTTGTAACCATGGCCTGCCTGCCTGTCTGTCTGTCTGCCAC CTGCCTGTGGTGTAACATAAAATTTTTGACTACCTTCTTTCTATGTTGTGGTCAAGCAATATGTGTTTAA GATCTGTTTTATCATATGATCGTATGTGTGTGTGTGCATGTGTCTTTACATGTGTGTGTACATGCACATG GGAGGGACATCAGAGTGCCTGAAACCCAAGTTACTGGCAGTTGTGAGCCCCCTGATGTGGGTGCTGGAAC CCAAACTCCAGTCTTAGGGAAGAGCAGCAAGAGAGTTCTTAACCACTAAGCTGTCTCTCCAGCCCCCTCA AGCAATATTTTTGATACACCATGTATTTTTACACTGCTATACTCTAAGCACCAGGGTTCCCAATATCCTG ATTTCTAAAAGGGAACCGAAGTAAAACAGATGCCTGACTGTTTTAGAAGATCTTTTGAATGTTTTATGAC TGCCTGCTTGTTTGAATATTGGCAAAAAGATAAATAATAAATTGACATCAAAAATACTAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3599134 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599134 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11573 same embryo
	EMAGE:11572 same embryo
	EMAGE:11574 same embryo
	EMAGE:11577 same embryo
	EMAGE:11575 same embryo
	EurExpress:euxassay_003646 same experiment
	MGI:4826735 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source