EMAGE mouse gene expression spatial database

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EMAGE:11592

Il2rb interleukin 2 receptor, beta chain ( MGI:96550)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006944. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006944_01	euxassay_006944_02	euxassay_006944_03	euxassay_006944_04

euxassay_006944_05	euxassay_006944_06	euxassay_006944_07	euxassay_006944_08	euxassay_006944_09

euxassay_006944_10	euxassay_006944_11	euxassay_006944_12	euxassay_006944_13	euxassay_006944_14

euxassay_006944_15	euxassay_006944_16	euxassay_006944_17	euxassay_006944_18	euxassay_006944_19

euxassay_006944_20	euxassay_006944_21	euxassay_006944_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11592_wholemount_strong.wlz

11592_wholemount_moderate.wlz

11592_wholemount_weak.wlz

11592_wholemount_possible.wlz

11592_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11592_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

spotted

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7093
Entity Detected:	Il2rb, interleukin 2 receptor, beta chain ( MGI:96550)
Sequence:	sense strand is shown >T7093 GCTACCTAAGCTGCGGAACAGGAGATGGCCGTGGACCCAACCTCCAGCAGGACTGCAGGGAACATCTCGA CACAACTCCATGTTGCAGCCAGGGTTTGCATCCTCAGCTCCTCTCAGCTGTGATGGCTACCATAGCTCTT CTCTGGAGCCTGTCCCTCTACGTCTTCCTCCTGCTCCTGGCTACACCTTGGGCATCTGCAGCAGTGAAAA ACTGTTCCCATCTTGAATGCTTCTACAACTCAAGAGCCAATGTCTCTTGCATGTGGAGCCATGAAGAGGC TCTGAATGTCACAACCTGCCACGTCCATGCCAAGTCGAACCTGCGACACTGGAACAAAACCTGTGAGCTA ACTCTTGTGAGGCAGGCATCCTGGGCCTGCAACCTGATCCTCGGGTCGTTCCCAGAGTCCCAGTCACTGA CCTCCGTGGACCTCCTTGACATAAATGTGGTGTGCTGGGAAGAGAAGGGTTGGCGTAGGGTAAAGACCTG CGACTTCCATCCCTTTGACAACCTTCGCCTGGTGGCCCCTCATTCCCTCCAAGTTCTGCACATTGATACC CAGAGATGTAACATAAGCTGGAAGGTCTCCCAGGTCTCTCACTACATTGAACCATACTTGGAATTTGAGG CCCGTAGACGTCTTCTGGGCCACAGCTGGGAGGATGCATCCGTATTAAGCCTCAAGCAGAGACAGCAGTG GCTCTTCTTGGAGATGCTGATCCCTAGTACCTCATATGAGGTCCAGGTGAGGGTCAAAGCTCAACGAAAC AATACCGGGACCTGGAGTCCCTGGAGCCAGCCCCTGACCTTTCGGACAAGGCCAGCAGATCCCATGAAGG AGATCCTCCCCATGTCATGGCTCAGATACCTTCTGCTGGTCCTTGGTTGTTTTTCTGGCTTCTTCTCCTG CGTCTACATTTTGGTCAAGTGCCGGTACCTTGGGCCATGGCTGAAGACAGTTCTCAAGTGCCACATCCCA GATCCTTCTGAGTTCTTCTCCCAGCTGAGTTCCCAGCATGGGGGAGACCTTCAGAAATGGCTCTCCTCGC CTGTCCCCTTGTCCTTCTTCAGCCCCAGTGGCCCTGCCCCTGAGATCTCTCCGCTGGAAGTGCTCGACGG AGATTCCAAGGCCGTGCAGCTGCTCCTGTTACAGAAGGACTCTGCCCCTTTACCCTCGCCCAGCGGCCAC TCACAGGCCAGCTGCTTCACCAACCAGGGCTACTTCTTCTTCCATCTGCCCAATGCCTTGGAGATCGAAT CCTGCCAGGTGTACTTCACCTATGACCCCTGTGTGGAAGAGGAGGTGGAGGAGGATGGGTCAAGGCTGCC CGAGGGATCTCCCCACCCACCTCTGCTGCCTCTGGCTGGAGAACAGGATGACTACTGTGCCTTCCCGCCC AGGGATGACCTGCTGCTCTTCTCCCCGAGCCTCAGCACCCCCAACACTGCCTATGGGGGCAGCAGAGCCC CTGAAGAAAGATCTCCACTCTCCCTGCATGAGGGACTTCCCTCCCTAGCATCCCGTGACCTGATGGGCTT ACAGCGCCCTCTGGAGCGGATGCTGGAAGGTGATGGAGAGGGGCTGTCTGCCAATAGCTCTGGGGAGCAG GCCAGTGTCCCAGAAGGCAACCTTCATGGGCAAGATCAGGACAGAGGCCAGGGCCCCGTCCTGACCCTGA ACACCGATGCCTATCTGTCTCTTCAAGAACTACAGGCCCAAGATTCAGTCCACCTAATATAGCAGGTGGC CAGGACTGGGATCCAGCTGCCTGGATCAGGTCAGGCTTGAGGAAGACTGCTTAAGAGGTCTCTTGAGGAC AGTCCACTGCTGAGGACTGTCCTGGCTATCCCCGCCCCCCCCCCAAACTTAATCATCCACTTCTGAACTC CATTTGCTACTTCCTGGTCTAACCAGGGTTTGGTGGAGGGTGGGGAGTGGGGGAGCGGTGGTCAGCTCCA CTGCCCTATTTAGTCATGAGGTCACTAGCTTCCTGTACCTGCCTCCCTCCCTCCTGCTTGCCTTCACAGG GCAGCTAGAACTTGCTTCCCCCAATACAAACATAACCTTGCTCCTCATTTATCCCTGAGCTTGCTGGGGC TCCTTACTTCCCTCTGCCCCATTTCCTGTCTCAACTCTTGCCTGCCAGCACGGCTGTGAAACAGCTGTCC TCAGGTTGTGATGTGGCTGTACACTGTCTTAAAATCAGAGCAGCTTTGACAACCCAAACGAAGAGGTCCT GATAAGACTGAATGCAGCCTGTCGACTATGGAGGGTCCCTCGTAGAAGGAGGGGTTGGGAAGGGTCATCA GTCATCACCTGAGATCGTAGCCACTCTCCTGCACTCCATCCTCAGCTATTCTGCTCCTGGAGCATGCGCT ATTTTATGTGTTCTTGGGCGATGCTGGTCTATGTAAGGGGTGGGGGGTGCTGATTGAAGTGAGGGCTCCT TCTATGTTGCTTTGGGGACCTTGTCTATTCTGGGGTGGGTTTTTCTGTGAAGCAGCTTCTTTGGGGTAAC CTAGCCCCCTTGTCAGTAAGCCTAAGAGAGTCAAAGGACCACCGAGCTGGACTTGAGTGTACGCCTACTT TTGATGCAACATCTGGGAAAAGTACTTTTTTTGTTAATGTATCCTTGAAGCTAAGAAAAGCTTACACCAC AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4015306 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4015306 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11593 same embryo
	EMAGE:11594 same embryo
	EMAGE:11595 same embryo
	EMAGE:11591 same embryo
	EurExpress:euxassay_006944 same experiment
	MGI:4825579 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source