EMAGE mouse gene expression spatial database

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EMAGE:11594

Slc35b4 solute carrier family 35, member B4 ( MGI:1931249)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006950. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006950_01	euxassay_006950_02	euxassay_006950_03	euxassay_006950_04

euxassay_006950_05	euxassay_006950_06	euxassay_006950_07	euxassay_006950_08	euxassay_006950_09

euxassay_006950_10	euxassay_006950_11	euxassay_006950_12	euxassay_006950_13	euxassay_006950_14

euxassay_006950_15	euxassay_006950_16	euxassay_006950_17	euxassay_006950_18	euxassay_006950_19

euxassay_006950_20	euxassay_006950_21	euxassay_006950_22	euxassay_006950_23	euxassay_006950_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11594_wholemount_strong.wlz

11594_wholemount_moderate.wlz

11594_wholemount_weak.wlz

11594_wholemount_possible.wlz

11594_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11594_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 17 18 19

pharyngo-tympanic tube

strong

regional

strong expression: see section 02 03 04 05 21 22 23 moderate expression: see section 01 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

urinary system

strong

spotted

strong expression: see section 06 07 22 23

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 18 19 20 21

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

right lung

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7111
Entity Detected:	Slc35b4, solute carrier family 35, member B4 ( MGI:1931249)
Sequence:	sense strand is shown >T7111 GTTGATCAAGACGGCCCTCAGGGAGGGAACCGCTGGCGGTAGTGATGTTGCGCGCGGATTTTCTAGGGTA GCGAACTGAAAGGGCGCTTTTTCTGCGCGCTGGCTCCCCGTGCCCCGCACCGGCCATGCGCCCGGCCTTC GCGGTGGGCCTGGTGTTCGCAGGCTGCTGCAGTAATGTGATCTTCCTAGAGCTGCTGGCCCGGACACATC CAGGATGCGGGAACATCGTCACGTTTGCGCAGTTCTTATTTATTGCTGTGGAAGGCTTCCTTTTTGAAGC TAACTTGGGAAGGAAGCCACCAGCTATCCCAATAAGGTACTATGCCATCATGGTAACCATGTTCTTCACT GTCAGCGTGGTGAACAACTATGCCCTGAACCTGAACATCGCCATGCCGCTGCATATGATATTCAGATCAG GCTCTCTGATTGCCAACATGATTCTAGGAATTATCATTTTGAAGAAAAGATACAGTATGTTCAAGTATAC CTCCATTGCTCTGGTGTCTGCAGGGATATTTATTTGCACTTTCATGTCAGCAAAGCAGGTGACTGTCCAG ACCGGCTTGAGTGACAAGGATGGATTCCAGGCCTTTGCATGGTGGTTACTAGGTATTGCGGCACTGACCT TCGCTCTGCTGATGTCGGCAAGAATGGGCATCTTCCAAGAGACTCTGTACAGACAGTTTGGGAAGCACTC CAAGGAGGCGTTATTTTATAATCACGCCCTTCCACTTCCCGGGTTCATCTTCTTGGCCTCTGATATTTAT GACCACGTTGTTCTGTTCAATAAGTCTGAACTCTATCAAGTTCCAGTCATCGGTGTGACGATGCCCATCA TGTGGTTCTACCTCCTCATGAATGTCGTGACTCAATATGTGTGCATCCGGGGTGTGTTCATCCTTACAAC AGAGTGTACCTCCCTCACCGTCACACTGGTTGTGACTCTGCGCAAGTTTGTGAGCCTCATCTTTTCTATC TTGTACTTCCAGAACCAGTTCACTATGTGGCACTGGCTGGGCACCTCGTTTGTCTTCATTGGAACCTTAA TGTACACAGAGGTGTGGAAAAACCTTGGGACCACAAAAAGTGAGCTGCAAAAGGATGACAAGAAGGACTG AGGTCTGCCTGGGACAGACAGACAGACGGTGTTATGTGAGAGCGGCCTCTGCTGCAGGTCTGGCCACCAT TTCACTTTAGCTGATGCCAAATTACCAGAGTATTGAAGCAGAAAGGCTCCAGAGCTTCCTCCAAAAGGAT GGGACTCATCTGCTCTCACGAGTCTGCATTTGTAGATTCCACATAGGCATCCCTGAGATAGAATAAACAA AACAAGCAAGGCTACCAGGCAGACCGTCGGGCAGCCTCTGCATTTGCTTTTCTATTTCTTTGCCAGAACA TCAGAACCTTATTTGTTGAATTCCAAGTCTCAGAGCTTCTAGTTTTCTACCTGTGTCGCACTTGTTGCTA CCGATGCCTTTTTGCTATGTACCTCTTTCCTCCCGTTACCCCTGCACACTTAAGCACTTTGCAGCACAGC CAACATTCCAGGCACAATCAGGCACTGAGTGATCCAGTGGACATGCGAAAAATTAGACCCCAGGAGTGAT GAGGCCGGACTCCCTCCAGCGTGTTGGTGTTGGGAGAGAAGCAGTTGTCCCCATGAGCAGAGCCCTTGAG ATCCGTGAGCTGGATGCTGGTGATGCTGGAGCAGACAGACGGCTGGATCCCACCACGCACATTTCACGTT ATTTGATTAGTCAATGCAAAGATCACACACTTCTTTCACCTGTTGCTTTCTGTAGTTAGAGCAGTCCTGC AGCGCTCATTGCACATTAGAAAGGAAGAAAACTTGAAAGTGGATCGAGAAGAGGGAGACCTTTCTTTCCA TCTCCCACGTGAAGCCCCTTGGCCTGTTTACGGAGATGGAGAAGGAAGTGTGGCCCAATACAGCTCACTC TTTCAGCACCCTGAGAGACTGTTGCCCCTGTGAACCCAGCCACACACTTCCATGGGACTCCTGTGGGTTT CAAATATGTGTCTGTGAGAGGCGTTAAGACCTGGTAGCCAGAAGATACTTCTGAGAATTGTCACCTGTCA AGTCATCGATGATGATTGATTGATACACAGGTGTGAGTGTGGTCAAACCCCAGCTGCCGCAGGGTGAAGT CTTCTTGTTCCATAGATATGGGACTGCTTTTGGGTGTGCCTGGGAGGTGGCATGACACAGCATGTTTACA TACAGAAACAGGCCTGGCTCTTTTCCGCTGATCACTCTGAAGGTCTGATTGTCCTCTCTTGGATCCCCTT GAGGAAGAGTAACCCTGTCACACAGCATGTGGCGCTTGTGTGCCCTTAGCTGGATTGTATGTGATTGAAG AACAAGAACATTGTGTTGCTTTCTTTCCTCGTCCCCACTTAGGGCCAGACCCTCATTACCCTCACCTAGC CATCTGCTCCTCTGCAAACAGCTACACACAATGTTTCTCTCGCACAGACGACAGCCTTCCATTCTTCCAC TATGTGCTGACTGTGCTCAAACTGAGGAGTGGCTCTCCATCTCCTCGGCTCACTGTAGCTCTTTGTTGCA CCCTCTGAAGATGCTCTTTGATGGGTCTGAACAGCAGCAGCAAAGAGAACTATCCCAATCCTCTGGGTCC CCTGGGAAGGCAAAGAGAACTGTCCTAATCCTCTGGGTCCCCTGGGAAGGCAGTTAAGACTTCTTTCTGT TACATTAAATGCAGATACTGTTTCTCCACCTAAGTCCCCAGGTTTGCAGACTGATTGTCATTCTGGCCAT TCACAGCTTCCCAGTGCTGGTAATGGTTTGTTCTCAAAGACCTGAATTAGATGTGAACCCATTCACAGTC CCAGAAGCTCTGTAGCTACTTTGAAATATGTGGGGTTGCCTGGACCAATGTTGCTATAAATACCAAGTGT TAAAAGCCCCTTAAAAAGACAGTAATAAAAAGTACTTTTTTTCTTCCAGTTTAACAGAAGAACCTGTCCC TCGGGGCTCTTGAGGCTCCATTCTGATTACTCCTAACGGCTATAACCTTCAGTTACCTAGAGCTTCCCTT TTTATGTTGGGAGAAGTACCACACAGCTTGTAGTTCCCTGAGACTGGCTGATACAATCTAACTGGACCGG TTAGGGTTTCAGAATGTGTATCCCTTTGTGAGTTTTGTTGAAGGAGATGTAAGAACAGATAAGAGTGCAG GCTCTGCTGGCTCAAGCTAGCAAGTGCCCCAGCTGCTTCCTGCCGTGCAGTAGACAGAAGAAAATGTAAC CGGCACTAGTTACAGTTTTGTAAAATTGCCCTTCTAAAGGTTCTGTTTTCCTTCTCATTGTGTATGCTTG TTGCACAGGGCCCTGTGGTTGGACACTTCCCTGCAGTTGAGTACTTCCCCCAAGCTCTCCTCTCCCCGTG TTAGGCCCTGCTGTTCCCCTGAACAGTTTTGCCTTCTACCTTCAGATCGCATACACACCATGGTTTCATG TCTCTCTACAAAGCTCAGGAGCTACAAATAAGAGCACACATATGCTGTTTGTCCTCCCTACACCAGCTAA CCCCATAATAGGACCATCTCTGGATACATGCATAAAATCACTCTTTATAAAAGTCATTTCTCTTTAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4017336 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017336 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11593 same embryo
	EMAGE:11592 same embryo
	EMAGE:11595 same embryo
	EMAGE:11591 same embryo
	EurExpress:euxassay_006950 same experiment
	MGI:4828199 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source