EMAGE mouse gene expression spatial database

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EMAGE:11682

Doc2g double C2, gamma ( MGI:1926250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008534. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008534_01	euxassay_008534_02	euxassay_008534_03	euxassay_008534_04

euxassay_008534_05	euxassay_008534_06	euxassay_008534_07	euxassay_008534_08	euxassay_008534_09

euxassay_008534_10	euxassay_008534_11	euxassay_008534_12	euxassay_008534_13	euxassay_008534_14

euxassay_008534_15	euxassay_008534_16	euxassay_008534_17	euxassay_008534_18	euxassay_008534_19

euxassay_008534_20	euxassay_008534_21	euxassay_008534_22	euxassay_008534_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11682_wholemount_strong.wlz

11682_wholemount_moderate.wlz

11682_wholemount_weak.wlz

11682_wholemount_possible.wlz

11682_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11682_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9941
Entity Detected:	Doc2g, double C2, gamma ( MGI:1926250)
Sequence:	sense strand is shown >T9941 GACACAGCTCTCAAGAGGTGTGTGCTCGGTGCCCCCAGTAGGTGGGAGCTTCTCTGAGTCCCTATGTCTG GACCACCATGGCATGTGCAGGGCCAGCCAGTGGGCGACAGCGGGTGAGCATGCAGGAACACATGGCCATC GATGTGAGCCCTGGCCCCATTCGGCCCATCCGCCTCATTTCCAACTACTTCCCACATTTCTACCCCTTCT TGGAGCCGGTGCTGCGTGCCCCAGATCGGCAGGCAATGCTGGCCCCAGCCATCCCCTCTGCACCCCAGCT GCAGCCCAACCCTGAGCCCGAAGGAGACTCGGATGACAGCACTGCCCTAGGCACCCTAGAATTCACACTT CTCTTTGATGAGGACAACAGCGCCCTGCACTGCACAGCCCATCGTGCAAAGGGTCTCAAGCCACCAGCCG CCGGCTCTGTGGACACCTATGTCAAAGCCAACTTACTACCAGGGGCCAGCAAGGCCAGCCAGCTTCGGAC ACGCACTGTTCGAGGCACCAGGGAACCTGTCTGGGAGGAGACTCTCACCTATCATGGCTTTACATGCCAG GATGCTGGACGGAAGACCCTGAGACTATGTGTGTGTGAGGACTCACGGCTGCGGCGCCGGCGGCGAGGAC CCCCCCTGGGGGAGCTACGAGTGCCCCTGAGGAAGCTGGTGCCAAACCGAGCCAGGAGCTTTGACATCTG TCTAGAGAAGCGGAAGCTGACCAAGAGGCCCAAGAGCCTGGACACAGCTCGTGGCATGTCTCTATATGAG GAGGAGGAGATGGAGGCAGAGGTGTTTGGGGAGGAGCGTGGCCGCATCCTACTGTCCCTGTGCTACAGTT CCGAGCGCGGTGGCCTGCTGGTGGGTGTGCTACGCTGTGTTCACCTTGCTCCCATGGATGCCAATGGCTA CTCAGACCCCTTTGTCCGCCTGTTCCTGCATCCAAGTTCTGGGAAGAAATCCAAATATAAGACCAGTGTT CGGAGGAAGACCCTGAACCCTGAGTTCAATGAGGAATTCTTTTATGCAGGTCATCGGGAGGAGCTAGCCC AGAAGGCACTGCTGGTGTCTGTGTGGGACTATGACCTGGGCACAGCTGATGATTTCATTGGTGGGGTGCA GCTGAGTGGCAGAGCCAGTGGGGAACGCCTGCGCCACTGGCGGGAGTGCTTAGGCCACTGTGACCACCGG CTGGAACTGTGGCACCTGCTGGACAGCGTGCCCCCCCAACTTGGTGACTAGTCAGCACAGCACAGGGCCC TGCTGTGCAACCAGCTCAGGAGCCAAGATGTCTGCTGCATCTGGAAAGAGGTCTGGGCTATCACATCTGC TATGCATTGTCTCCTTCCAGTCATGTAACCCCCAAACACAAAGCAAAATAAACACCTGCTTCTGCCAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30296398 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30296398 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11683 same embryo
	EMAGE:11680 same embryo
	EMAGE:11684 same embryo
	EMAGE:11681 same embryo
	EurExpress:euxassay_008534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source