EMAGE mouse gene expression spatial database

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EMAGE:11683

Pcdhgc4 protocadherin gamma subfamily C, 4 ( MGI:1935203)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006964. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006964_01	euxassay_006964_02	euxassay_006964_03	euxassay_006964_04

euxassay_006964_05	euxassay_006964_06	euxassay_006964_07	euxassay_006964_08	euxassay_006964_09

euxassay_006964_10	euxassay_006964_11	euxassay_006964_12	euxassay_006964_13	euxassay_006964_14

euxassay_006964_15	euxassay_006964_16	euxassay_006964_17	euxassay_006964_18	euxassay_006964_19

euxassay_006964_20	euxassay_006964_21	euxassay_006964_22	euxassay_006964_23	euxassay_006964_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11683_wholemount_strong.wlz

11683_wholemount_moderate.wlz

11683_wholemount_weak.wlz

11683_wholemount_possible.wlz

11683_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11683_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9123
Entity Detected:	Pcdhgc4, protocadherin gamma subfamily C, 4 ( MGI:1935203)
Sequence:	sense strand is shown >T9123 CGCGAAAAGGGGCGGGGTGAATTGATGTGTTAGAGGGGGAATACATATATGATTTCTGCCTCTCCACGAT GATAACGAGCCCCGGACACAAATGCGAACTGAACAGAAGGAAGGTCGAAAGAAACGTTGGTATTTATTAT GTTGGGGGGTTTGTTTGGCGGGGGCTGCATGTGTGTGTTTCCTCCCAATGCTACGACGCATTAACCCTGC TGCTATTGGGACATTCTCTTCTCAGCCTATTGGCTGAGCCCAGGAGTTGCTGCCTGCCAATCGGGTAATG AAAGGCAGACAAATCTACCCCGCCACCAGCAAAAAACGGCGCGTAACCCTTGCAGCGCCGGCGGAGCCGC GCCAGACCTGGCGCGGGGAAGGGAGATAGGTGTCTCCAGCTGCTGTGGCTGTTAGGGGCGGGTCAGCTTC TTTGGCGTCTCCGGACCAAGTAGAGAATTAGCGGATGGCAGCAATGCCCAGCACGGTGAGAAGCTGGGTA GAAATTTGGTGGGGGGCTACCCTTTTGTTCCTCTTTTGCCACCTGGGCTACGTTTATGGGCAGATTCGCT ACCCGGTCCCAGAGGAGTCACAGGAAGGGACTTTTGTAGGGAATGTCGCCCAAGATTTTTTGCTGGATAC AGAGAGTCTATCCGCTCGCAGGCTGCAGGTCGCTGGAGAGGTGAACCAAAGACACTTCCGTGTGGATTTG GACAGCGGAGCCCTGCTCATCAAAAATCCAATCGATCGAGAGGCACTGTGTGGGCTCAGTGCCAGCTGCA TCGTGCCCCTGGAGTTTGTAACCGAAGGGCCTTTGGAAATGTACCGAGCGGAGGTTGAGATCGTAGATGT GAATGATCACGCCCCCCGATTTCCGCGGCAGCAGTTGGACTTGGAAATCGGGGAAGCAGCTCCGCCAGGA CAGCGTTTCCCCTTGGAAAAGGCTCAGGATGCAGATGTTGGGAGCAATTCCATTAGCAGCTACAGACTAA GCTCCAATGAGCACTTTGCCCTGGATGTCAAGAAGCGCAGCGACGGCAGCCTGGTCCCAGAGCTTCTTCT GGAGAAGCCCTTGGATCGTGAGAAGCAATCCGACTACCGCCTGGTGCTGACTGCTGTAGATGGAGGGAAC CCCCCTAGATCTGGAACCGCAGAGCTCCGGGTGTCAGTGCTGGATGTGAATGACAACGCCCCAGCCTTCC AGCAATCCAGTTACAGGATCAGCGTGTTGGAGAGCGCGCCAGCGGGCATGGTGCTCATCCAGCTCAATGC CTCTGACCCAGACCTGGGACCTAGTGGTAACGTCACCTTTTCTTTTAGTGGTCACACCCCTGACCGTGTG AGAAACCTCTTTAGCCTTCATCCCACTACTGGAAAGCTTACCCTTCAGGGGCCTCTAGACTTTGAGAGTG AGAATTATTACGAATTTGATGTAAGGGCTCGTGATGGGGGTTCTCCAGCTATGGAGCAACACTGCAGCCT TAGGGTGGATCTTCTAGATGTGAACGACAACGCCCCCCATATCACAGTGACCTCTGAGCTTGGAACTCTA CCAGAGAGTGCAGAACCTGGCACTGTGGTAGCACTAATCAGTGTGCAGGACCCTGACTCGGGGTCAAATG GGGATGTGAGCCTACGGATTCCCGACCACTTACCATTTGCCCTCAAGTCTGCCTTCAGGAACCAGTTCTC CCTCGTGACAGCAGGACCCTTGGACCGAGAGGCCAGATCCAGTTACGACATCATGGTTACCGCTTCTGAT GCTGGGAACCCTCCGCTGAGCACCCACAGGACGATTTTCCTCAATATCTCAGATGTGAATGATAACCCAC CCTCATTCTTTCAGAGGTCGCATGAGGTGTTTGTTCCTGAGAACAATCGCCCAGGGGACCTGCTTTGCTC CCTTGCAGCCTCTGACCCAGACTCTGGCTTAAACGCGTTAATCTCATACTCTCTCTTAGAGCCCAGAAAC CGAGACGTATCGGCTTCTTCCTTCATCTCTCTGAACCCCCAGACAGGGGCCGTTCATGCTACTAGATCAT TTGACTATGAACAAACACAGACACTGCAGTTTGAGGTGCAGGCCAGGGACAGGGGCAGCCCACCTCTCAG CAGCACTGTGACAGTGCGCCTGTTTGTTCTAGACCTCAATGACAATGCCCCAGCAGTGCTACGCCCCAGG GCCAGGCCTGGTTCCTTATGTCCCCAGGCGTTGCCTCCATCAGTTGGCGCTGGTCACCTCGTCACAAAGG TGACGGCTGTGGACTTAGATTCAGGTTACAATGCTTGGGTTTCCTATCAGCTCCTGGAGGCCCCAGATCC CAGCCTGTTTGCAGTCTCCCGTTATGCAGGGGAAGTACGGACTGCTGTTCCCATCCCAGCTGACCTCCCA CCGCAGAAGCTGGTCATAGTGGTGAAGGACAGTGGTAGCCCACCACTCTCTACCTCTGTGACTCTCCTAG TGTCCTTAGAGGAAGACACTCATCCAGTTGTCCCCGATCTTCGAGAATCTTCAGCTCCCAGGGAAGGAGA ATCCCGCCTAACCCTCTACTTGGCTGTGTCCTTAGTGGCGATCTGCTTTGTCTCCTTTGGTTCTTTTGTG GCACTGCTCTCTAAGTGTCTTCGTGGGGCTGCCTGTGGAGTAACGTGCTTTCCTGCTGGCACCTGCGCCT GTCTCACCAGATCTCGGAGGAGGGAGGGGCTTCCTCCTTCCAATGGGATCCTCCGAATCCAGCTAGGGTC AGAAGATCCTATCAAGTTTGTTGACGTGGGAGGCCACTCTCATGGCTGTACACCATTGGCTTCTGCGCCT ACTCGGAGTGATAGCTTCATGATGGTGAAGTCACCCAGTGCACCTATGGCTGGGGAGCCCGTGCGGCCAA GCTGTCCACCCTCTGATCTTCTCTATGGGCTAGAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCA AGCCCAGAGACCCGGCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTT GATACAGAGATGCTGCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGG GAGGGGGCGCTGGCACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGA CTACCGCCAGAACGTGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGC AAGGCTCCAGCAGGTGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCC AGGCCTTGAACCACAAGGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCA GAATTTCAGGGCTCACCCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACAC GTGCCCAAGCCAACACCCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCC CCCTATTCAATCAGTGATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACA CACACACACACACACAAACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGG CAGGCTTATCCATCTCCTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGG TAGCTGGGCTGTTGGGGTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACA ATCTTTTGGCCTCTCCTTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTT CCAGAGCCTGAGACCAATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGC TGGATTTTGGGAAGGCATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTT CATTGTTTGATGTTCCCACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCT GGAGACAGTAGGGGGTGGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACA AAACAGCCTGTTAATAGGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATT CTAGCCATAGACAAACACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCAC CAGGACCAATGGATTTAAGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGA GGAAAGAGGGGCCTCTGTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGC AGGAAGGGGGGCCCAGCAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAG TGGCCATAAAGTCCCAGCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTC CACCTTACCAGGTGCCATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAG AGAACGGTTAAAGCCCCCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGT AGAAGATCCCCCTCCCCCGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCG AGTGGGCAGCGGGCGGTGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGT GCTGTGTTTTGTGCTGTGTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTC TCTTCGCATAGTCACGTAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATAT TTATATGGCTTTTTTCTTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825007 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825007 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11680 same embryo
	EMAGE:11684 same embryo
	EMAGE:11681 same embryo
	EMAGE:11682 same embryo
	EurExpress:euxassay_006964 same experiment
	MGI:4827092 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source