EMAGE mouse gene expression spatial database

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EMAGE:11753

Hgd homogentisate 1, 2-dioxygenase ( MGI:96078)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007117. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007117_01	euxassay_007117_02	euxassay_007117_03	euxassay_007117_04

euxassay_007117_05	euxassay_007117_06	euxassay_007117_07	euxassay_007117_08	euxassay_007117_09

euxassay_007117_10	euxassay_007117_11	euxassay_007117_12	euxassay_007117_13	euxassay_007117_14

euxassay_007117_15	euxassay_007117_16	euxassay_007117_17	euxassay_007117_18	euxassay_007117_19

euxassay_007117_20	euxassay_007117_21	euxassay_007117_22	euxassay_007117_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11753_wholemount_strong.wlz

11753_wholemount_moderate.wlz

11753_wholemount_weak.wlz

11753_wholemount_possible.wlz

11753_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11753_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4533
Entity Detected:	Hgd, homogentisate 1, 2-dioxygenase ( MGI:96078)
Sequence:	sense strand is shown >T4533 CCACGCGTCCGGTTTTAAAGAGACACGGGAACATTGGTACCTCACAAACTCCTTGGCCTGGGTCTCCCTC CAGCAGAAGGGAAATGGCAGAGTTAAAGTACATTTCTGGATTTGGGAATGAGTGTGCTTCAGAGGATCCT CGCTGTCCAGGTTCCCTGCCCAAAGGACAGAATAATCCTCAGGTCTGCCCCTACAACCTCTACGCTGAGC AGCTCTCGGGATCAGCCTTCACTTGTCCACGGAACACCAATAAGAGAAGCTGGCTGTATAGGATTCTTCC TTCAGTTTCTCACAAACCCTTTGAGTCCATCGACCAAGGCCATGTCACTCACAACTGGGATGAAGTTGGC CCTGATCCTAACCAGCTAAGATGGAAACCATTTGAGATTCCAAAAGCGTCTGAGAAGAAAGTGGACTTTG TGAGTGGTTTGTACACCTTATGTGGAGCAGGAGACATCAAGTCTAACAACGGCCTTGCTGTGCACATTTT TCTGTGTAACTCCTCCATGGAGAGCAGGTGCTTTTACAACTCAGATGGGGATTTCCTGATTGTTCCCCAG AAAGGGAAACTTCTCATTTACACCGAGTTTGGCAAGATGTCCCTGCAGCCCAATGAAATCTGCGTCATTC AGAGAGGAATGCGATTCAGCGTCGATGTCTTTGAGGAGACCAGGGGCTACATCCTGGAGGTCTATGGTGT CCACTTTGAGTTACCTGACCTGGGACCCATCGGAGCAAATGGCTTGGCCAATCCTCGAGATTTCTTGATT CCCGTTGCCTGGTATGAAGATCGTCGAGTGCCAGGTGGTTACACTGTGATTAATAAATTCCAGGGCAAGC TGTTTGCTTGCAAACAGGATGTCTCTCCATTCAATGTCGTGGCCTGGCATGGAAACTACACACCCTACAA GTACAACCTAGAGAACTTCATGGTCATCAATGCGGTAGCCTTTGACCATGCGGATCCTTCCATTTTCACC GTGCTGACTGCCAAATCCCTCCGACCTGGGGTGGCTATTGCTGACTTTGTCATCTTCCCACCTCGGTGGG GAGTTGCAGATAAAACCTTCAGACCTCCTTATTACCACAGGAACTGCATGAGTGAGTTCATGGGCCTCAT CAAAGGTCACTATGAGGCAAAGCAAGGTGGATTCCTGCCAGGGGGCGGCAGCCTGCACAGTGCCATGACC CCTCACGGCCCCGATGCAGACTGCTTTGAAAAGGCCAGCAAGGCCAAACTCGAGCCAGAGAGGATAGCTG ACGGCACCATGGCGTTTATGTTTGAATCTTCCTTAAGTTTGGCGGTCACCAAGTGGGGACTCAAGACCTG TAGCTGTTTGGATGAGAACTACTACAAATGTTGGGAGCCTCTCCGGAGCCACTTCACCCCCAATTCCAGG AGCCCGACAGAGCCTAAGTGAGACTGGAGCCCTCTACTGTAATTGAGAACAGACTTGCATAGCTTCCTTG AGATCCTCACAGCTCTGATGCTACAGGGACAGCGATTGAAATGAGAGCTCACTTGTCATAGTCAAGAAAC TCAGAATGTTTGTGGACATGCATTCAGAAATGCCTATGGCTTCCTAAATTACTCAATAAATCTGTGTTCT GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4973560 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4973560 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11754 same embryo
	EMAGE:11750 same embryo
	EMAGE:11752 same embryo
	EMAGE:11749 same embryo
	EMAGE:11751 same embryo
	EurExpress:euxassay_007117 same experiment
	MGI:4825367 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source