EMAGE mouse gene expression spatial database

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EMAGE:11754

Plekha3 pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 3 ( MGI:1932515)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007085. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007085_01	euxassay_007085_02	euxassay_007085_03	euxassay_007085_04

euxassay_007085_05	euxassay_007085_06	euxassay_007085_07	euxassay_007085_08	euxassay_007085_09

euxassay_007085_10	euxassay_007085_11	euxassay_007085_12	euxassay_007085_13	euxassay_007085_14

euxassay_007085_15	euxassay_007085_16	euxassay_007085_17	euxassay_007085_18	euxassay_007085_19

euxassay_007085_20	euxassay_007085_21	euxassay_007085_22	euxassay_007085_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11754_wholemount_strong.wlz

11754_wholemount_moderate.wlz

11754_wholemount_weak.wlz

11754_wholemount_possible.wlz

11754_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11754_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 18 19 20 21 22 23

submandibular gland primordium

moderate

regional

moderate expression: see section 06 16 17 18 weak expression: see section 07 08 09 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 weak expression: see section 10 13 14 15 16 21

mandible

moderate

regional

moderate expression: see section 02 03 04 05 weak expression: see section 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

lower jaw incisor

weak

regional

weak expression: see section 09 10 11 12 14 15

maxilla

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07 16 17 18 19 20 21

upper jaw incisor

weak

regional

weak expression: see section 09 10 11 14 15

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 20 21 weak expression: see section 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4564
Entity Detected:	Plekha3, pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 3 ( MGI:1932515)
Sequence:	sense strand is shown >T4564 GGTCAATGAAAACAAGCCGGGCCTCTGAGGGCCGCAGCAGCGCGGCCGGCGCCGGGCCAGGAGGATGCGC GGCGCCGGGCTCTGAAGCATGGAGGGGGTTCTGTACAAGTGGACCAACTATCTCACAGGTTGGCAGCCTC GATGGTTTGTTCTGGATAATGGAATCCTGTCCTACTATGACTCACAGGATGATGTCTGCAAAGGGAGCAA AGGGAGTATAAAGATGGCGGTCTGTGAGATTAAAGTCCATCCCGCAGACAACACAAGAATGGAGTTAATC ATTCCAGGAGAGCAGCATTTCTACATGAAGGCAGTAAATGCCGCCGAGAGACAGAGGTGGCTGGTTGCCC TTGGGAGCTCCAAAGCGTGTTTGACCGACACGAGGACTGCAAAAGAGAAAGAAATAAGTGAGACCAGTGA ATCTCTGAAAACCAAAATGTCTGAACTTCGCCTCTACTGTGACCTCCTGATGCAGCAGGTTCATACGATC CAGGAATTCGTCCACCGTGATGAGAGGCATCCCTCTCCCAGTGTGGAGAACATGAATGAAGCCTCCTCCT TGCTCAGTGCCACCTGTAACACATTCATCACAACCCTGGAGGAGTGTGTGAAGATCGCCAACGCCAAGTT TAAACCTGAGATGTTTCAACTGCCTCATCCGGATCCCCTGGTCTCTCCCGTGTCGCCTTCTCCTGTTCAG ATGATGAAGCGTTCAGCCAGCCACCCTGGTTCCTGCAGTTCCGAGAGGAGCAGCTGCTCCATCAAAGAAC CAGCATCTGCCCTCCACCGACTTCCTCAGCGACGCCGCAGAACCTACTCGGACACAGACTCTTGTAATGA TGTTCCCCCTGAAGACCCAGAGAGACCTCTTCACTGTTCAGGAAACACACTTAATGGAGATTTGGCATCA GCAACCATTCCGGAAGAAAGCAGACTCATGGCCAAGACACAATCTGAAGAACCTCTTCTGCCCTTCTCCT GAGGAAACAGACATGCCCAGCTTCCTCCTGAGGAAACAGACATGCCCAGCTTCCTCCTGAGGAAACAGAC ATGCCCAGCTTCCTCCTGAGGAAACAGACATGCCCAGCTTCCTCTGAGTGTCGCTATGCAAAAGCTGCTG TAATTAAACTCGGTCTGGGCTAGCTTTGCCCTCTCCTTAGGATTTCTCTGCACTTTATAGAATATTGTAA ACAAACAACCCACATACTTTTGAAGTGTATTTTATCTTTCTATAGTTTACTTGCAAGAGTATTTTCCTAA TAACTTCACAGTATGAATGTGCATCTTTTTTTTTTTTTTAAACAAATGATGGTGTAACATTTTGACATCC ATAAGGACAAATGTAGATATTTTTCTAAAAAACTGTGAGGGACTGACAGCTTGGTCAGTGTGTATTGTAG TATATAAACATGAAATCTCGCCAGATTTATTTGACAGAAATGTGAGAGATGTAACTTGTGCCATGGACCA AAAGGTCACTTCACCCCAGCTTAAAATTAATTACCATAGCAGCTTGATGGTGATTATATCATATTCCTTT AAGCAAAAAGGAAACGCTTAATATTCTAAAGGTCTTTAGCCCAAATACCATGACATATTGAGCATTTTTT TTTAAAAAGCAGACTCCGCTGTCCTTCATATGTGAAGTTGACATCTACTGATTTGTCAATACCAAACATC AGATTACAGTATTTAATTTTTATTTATTTATTTTCTTATTGCATCAGAAGATGGTTATGTCCTAACTTTT ATGGCCTCCCCAATTTAAGATGTATATGCATAGTTGTTATTACGTTGTTCTAAGATACATGAGGCAAGTG TCCCAGTGATCTTGTTCCCTTACACGAGAGAAGTAAACAGCTTTCAATGGGAATGGAGTTCAGTGCTTTT CAGAAAATAGGCAGCAAGCTGCTGTTGTAAGGTATGATTTGCAGCTCTTTGGCATATCTAGAGACATTTT TAATTTATGAATATTTATACAAAAAGCAATTCTGTCAAGATGACTGTTCTATATCACTTGAGAATGGCAT TATTTAATTAAAGAACAATTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4981486 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4981486 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11750 same embryo
	EMAGE:11752 same embryo
	EMAGE:11753 same embryo
	EMAGE:11749 same embryo
	EMAGE:11751 same embryo
	EurExpress:euxassay_007085 same experiment
	MGI:4827264 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source