EMAGE mouse gene expression spatial database

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EMAGE:11879

Ltbp2 latent transforming growth factor beta binding protein 2 ( MGI:99502)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011131_01	euxassay_011131_02	euxassay_011131_03	euxassay_011131_04

euxassay_011131_05	euxassay_011131_06	euxassay_011131_07	euxassay_011131_08	euxassay_011131_09

euxassay_011131_10	euxassay_011131_11	euxassay_011131_12	euxassay_011131_13	euxassay_011131_14

euxassay_011131_15	euxassay_011131_16	euxassay_011131_17	euxassay_011131_18	euxassay_011131_19

euxassay_011131_20	euxassay_011131_21	euxassay_011131_22	euxassay_011131_23	euxassay_011131_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11879_wholemount_strong.wlz

11879_wholemount_moderate.wlz

11879_wholemount_weak.wlz

11879_wholemount_possible.wlz

11879_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11879_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

upper arm mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 moderate expression: see section 20 21 22 23 24

upper leg mesenchyme

moderate

regional

moderate expression: see section 20

anterior naris

strong

regional

strong expression: see section 14 15 16 17 18

external naris

strong

regional

strong expression: see section 14 17

heart valve

strong

regional

strong expression: see section 10 11 12 13 14

upper lip

strong

regional

strong expression: see section 15 16 17 18

thyroid cartilage

strong

regional

strong expression: see section 12 13 14 15

axial skeleton

strong

regional

strong expression: see section 10 11 12 13

basioccipital bone

strong

regional

strong expression: see section 11 12 13 14

basisphenoid bone

strong

regional

strong expression: see section 11 12 13 14

orbito-sphenoid

strong

regional

strong expression: see section 03 04 05 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36601
Entity Detected:	Ltbp2, latent transforming growth factor beta binding protein 2 ( MGI:99502)
Sequence:	sense strand is shown >T36601 AGATGCCGATGAATGTGTACTGTTTGGGCCTGCTCTCTGCCAGAATGGCCGATGCTCAAACATAGTGCCT GGCTACATTTGCCTGTGCAACCCTGGCTACCACTATGATGCCTCCAGCAGGAAGTGCCAGGATCACAACG AATGCCAGGACTTGGCCTGTGAGAACGGTGAGTGTGTGAACCAAGAAGGCTCCTTCCATTGCCTCTGCAA TCCCCCCCTCACCCTAGACCTCAGTGGGCAGCGCTGTGTGAACACGACCAGCAGCACGGAGGACTTCCCT GACCATGACATCCACATGGACATCTGCTGGAAAAAAGTCACCAATGATGTGTGCAGCCAGCCCTTGCGTG GGCACCATACCACCTATACAGAATGCTGCTGCCAAGATGGGGAGGCCTGGAGCCAGCAATGCGCTCTGTG CCCGCCCAGGAGCTCTGAGGTCTACGCTCAGCTGTGCAACGTGGCTCGGATTGAGGCAGAGCGCGGAGCA GGGATCCACTTCCGGCCAGGCTATGAGTATGGCCCTGGCCTGGACGATCTGCCTGAAAACCTCTACGGCC CAGATGGGGCTCCCTTCTATAACTACCTAGGCCCCGAGGACACTGCCCCTGAGCCTCCCTTCTCCAACCC AGCCAGCCAGCCGGGAGACAACACACCTGTCCTTGAGCCTCCTCTGCAGCCCTCTGAACTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74672. Forward Primer - name:074672_F_cDNA_Ltbp2, sequence:AGATGCCGATGAATGTGTACTG; Reverse Primer - name:074672_N_SP6_cDNA_Ltbp2, sequence:GAAGTTCAGAGGGCTGCAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11875 same embryo
	EMAGE:11874 same embryo
	EMAGE:11876 same embryo
	EMAGE:11878 same embryo
	EMAGE:11877 same embryo
	EurExpress:euxassay_011131 same experiment
	MGI:4826018 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source