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EMAGE:11945

Mcpt8 mast cell protease 8 ( MGI:1261780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945
"Pseudo-wholemount" of euxassay_011102. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011102_01 euxassay_011102_02 euxassay_011102_03 euxassay_011102_04
EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945
euxassay_011102_05 euxassay_011102_06 euxassay_011102_07 euxassay_011102_08 euxassay_011102_09
EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945
euxassay_011102_10 euxassay_011102_11 euxassay_011102_12 euxassay_011102_13 euxassay_011102_14
EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945 EMAGE:11945
euxassay_011102_15 euxassay_011102_16 euxassay_011102_17 euxassay_011102_18 euxassay_011102_19
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euxassay_011102_20 euxassay_011102_21 euxassay_011102_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11945Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11945_wholemount_strong.wlz
11945_wholemount_moderate.wlz
11945_wholemount_weak.wlz
11945_wholemount_possible.wlz
11945_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11945_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36625
Entity Detected:Mcpt8, mast cell protease 8 ( MGI:1261780)
Sequence:sense strand is shown

>T36625
GGGAAATCATATGGGGTACAGAGTCCAAACCCCACTCCCGGCCCTATATGGCATACATAAGGTTTAATGA
CAGCAAATCAGTCTATCGCTGTGGTGGTTTCCTGGTGGCAAGAGACATCGTAATGACAGCAGCTCACTGT
AATGGAAAAGTTATAAATGTAACCTTAGGAATTCACAATCTCAAGAAAAAGAAAAACACCCAGCTCATTC
CTGTCAGTGAAGCCATACCTCACGAGAGCTTTGACAATGAAACACTGGTCAATGACATCATGCTCCTGAA
GCTGGAACGCAAAGCTCAACTCAACAGTGCTGTGGATACTATTGCCCTTCCAAAGAGCAAGGATTGGGTG
AAACCTGGGCAGGTGTGCACAGTGGCAGGTTGGGGAAAGTTGGCCAATTGCACTTTGTCTGACACACTTC
AAGAAGTGAATCTAGAAGTTCAAAAGGGCCAGAAGTGTCGCAGCATGTCCCAAACCTACAACGACTCCAT
CCAGCTCTGTGTGGGAAATCCCAGTGAGAATAAGGCTACTGGTAAGGGTGACTCTGGCGGACCCTTTGTG
TGTAATGGGGTGGTCCAGGGCATTGTCAGTTGTCGCTTGTGTACTGGGACACTTCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73872. Forward Primer - name:073872_F_cDNA_Mcpt8, sequence:GGGAAATCATATGGGGTACAGA; Reverse Primer - name:073872_N_SP6_cDNA_Mcpt8, sequence:GAGGAAGTGTCCCAGTACACAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11944 same embryo
 EMAGE:11946 same embryo
 EMAGE:11942 same embryo
 EMAGE:11943 same embryo
 EMAGE:11941 same embryo
 EurExpress:euxassay_011102 same experiment
 MGI:4826136 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS