EMAGE mouse gene expression spatial database

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EMAGE:11978

Tbc1d24 TBC1 domain family, member 24 ( MGI:2443456)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010949. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010949_01	euxassay_010949_02	euxassay_010949_03	euxassay_010949_04

euxassay_010949_05	euxassay_010949_06	euxassay_010949_07	euxassay_010949_08	euxassay_010949_09

euxassay_010949_10	euxassay_010949_11	euxassay_010949_12	euxassay_010949_13	euxassay_010949_14

euxassay_010949_15	euxassay_010949_16	euxassay_010949_17	euxassay_010949_18	euxassay_010949_19

euxassay_010949_20	euxassay_010949_21	euxassay_010949_22	euxassay_010949_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11978_wholemount_strong.wlz

11978_wholemount_moderate.wlz

11978_wholemount_weak.wlz

11978_wholemount_possible.wlz

11978_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11978_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 05 06 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 17 18 19

spinal cord

strong

regional

strong expression: see section 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18

retina

moderate

regional

moderate expression: see section 01

neural retina

moderate

regional

moderate expression: see section 02 03 04

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9352
Entity Detected:	Tbc1d24, TBC1 domain family, member 24 ( MGI:2443456)
Sequence:	sense strand is shown >T9352 CCGAAGCTGGGGCTGCAGGATTTAGCTGCTTTTTGTTCCCTTCCTGGCAGCCTAGGACCTGCCTTGTGGG CACACTGGGCACCATGGACCCCCCAGGGTACAATTGCTTTGTGGATAAGGACAAGATGGATGCATCCATC CAGGACCTGGGGCCAAAGGAGCTGAACTGCACGGAGCTACAGGAGCTGAAGCAACTGGCACGGCAGGGTT ACTGGGCTCAGAGCCACACCCTGCGCGGGAAAGTGTACCAGCGCCTGATCCGGGACATCCCCTGCCGCAC AGTCACACCTGATGCCAGCGTGTACAGTGACATTGTGGGGAAGATTGTGGGCAAGCACAGCAGCAGTAGT CTGCCCTTGCCTGAGTTTGTAGACAACACTCAGGTGCCCACCTACTGCCTGAACACACGGGGTGAAGGGG CTGTGCGCAAGATCCTCCTGTGTATTGCCAACCAGTTCCCTGACATCTCCTTCTGCCCTGCCCTGCCTGC TGTGGTGGCCTTGCTACTGCACTACAGCATCGATGAAGCTGAGTGTTTCGAAAAAGCCTGCCGCATCTTA TCCTGCAATGACCCCACCAAGAAGCTCATTGACCAGAGCTTCCTGGCCTTTGAGTCTTCCTGTATGACAT TTGGGGACCTGGTGAACAAGTACTGCCAGGCAGCCCATAAACTGATGGTGGCCGTGTCAGAGGACGTCCT GCAGGTCTACTCTGACTGGCAGCGATGGCTCTTTGGGGAGCTGCCCCTCAATTACTTTGCCCGTGTTTTT GATGTCTTCCTTGTAGAAGGCTACAAAGTATTGTACCGAGTTGCTCTGGCCATCCTCAAGTTCTTCCACA AGGTGAGAGCAGGACAGCCCCTTGAGTCAGACAATGTAAAGCAAGACATCCGAATGTTTGTCAAGGACAT TGCCAAGACAGTGTCCCCTGAGAAACTACTAGAGAAGGCCTTTGCCATCCGCCTATTTTCCCGAAAGGAG ATCCAGCTCTTGCAAATGGCCAATGAGAAAGCACTGAGGCAGAAGGGTATAACTGTCAAACAGAAGAGGC AGTTTGTGCACTTAGCTGTCCATGCAGAGAACTTCCACTCAGAGATTGTCAGCGTGAAGGAGATGAGAGA CATTTGGTCCTGGATCCCTGAGCGCTTTGCCCTCTGCCAACCCCTCCTGCTCTTCTCATCACTGCAGCAT GGGTACAGCCTAAGCAGGTTCTATTTCCAGTGTGAAGGACATGAGCCCACCCTCCTGCTCATCAAGACCA CTCAAAAGGAGGTCTGTGGGGCTTACCTGTCAACAGACTGGAGCGAGAGGACTAAATTCGGAGGCAAGCT GGGCTTCTTTGGGACTGGAGAATGCTTTGTTTTTAGGCTGCAGCCGGAAGTGCAGCGCTATGAGTGGGTG GTCATCAAACACCCAGAGCTGACCAAGGCAACATCCCTCAAGTCTTCAGAGGCTGCAGGCAGTTCTTCCC TCATCAGCCACTGCTCTTCAGACCCCGCTGATCGGCTCTCCCCTTTCCTGGCTGCCCGGCACTTCAACCT GCCCTCCAAGACTGAGTCCATGTTCATGGCTGGAGGCAATGACTGCCTCATCATTGGGGGAGGAGGTGGT CAAGCACTCTACGTTGATGGGGACCTGAATCGAGGGCGCACTGGACACTGTGACACATTCAACAACCAGC CCCTCTGCTCTGAGAACTTCCTCATCGCAGCTGTGGAGGCTTGGGGCTTCCAAGACCCTGACACCGAATG ACGGGCCCGCACTGATGGTGACAGCCACATTGTGGGGTGATGGGACAGAGGCCCGGCTGCCTCTTCAGGG AGCAGGGAGTGAGTGAGACGTCACCCCCAGAAAGTGGAAAGGTTTGCTGTGCAGTGGGGCTGTTAGTCTC TCACGCCAGGCCTGGCCCTGGCTTGGACTTAGGTTAGGATTCCTGAGCCCTCCTGCTTCTGAGCTCTTGA ACCCTCAGCCCTGTCTAGCATCTCTGTAAGAGACACCCTCTGCCCTCACCCAGGGCCCTGGCTCTGCCAG GTGTCCTGTACCCACAATTGCCAAGGCTACATTTCCATGCCCCTGAGCCTCAGCCCCTTATAAGAAAAGG GCAAATGAATCTTCTTGCCAAATGTGGGCCCATGGTGCCCCCCTGCTGGTGCTTTGACCCTGCAGCTCCT CTGAGGTTCCCCTCAGAGGGGTACATTTGCTGCTGTAGTGTAGGGTAGGATTGGGAAGGGCAGGGTCTGT GAGTTCTGCAGCTCCCCCTAGTCTAGAGATGGAATGAGGGCTGGAGAACTGTCTTTTGTCTTTGTGTTTC TGCAGAGCCTGAAGCTCTTGCTGCGCCTCGCTGCGAGTCCACACCTGAAGGTGGCCTCCGGAGAGACTTT CTTTGACCACCTTGGCCAGTCAGCTCCATTTCCAGAGTTCTTCTCAGAGCTGACTAGACTCTGAGGCCTT TGCCTCTCATCCCTGTGGCCTCCTTGAAGTTCTGTAGTCTTGGTGACTCTTGTACTTGGTTCCTCTGTCA CAGTGACTGAGGCCCACCAAGCATGGCATGCTTGTCCCCATCCAGCTTTTTGCCTTCTCCCCAGGCCCTG TGCTCCCCTGGACCCGTTCACACAGCATCTGATTCACCTGTGTGACCTGATCCCTCAGCTGGCACTGTGG CCGACCACGTACTGTATCTTCTGTGTGCTACCCCTGCCCCTATTAAGGCCTTCAGGTCTCTGGATGTCTG TGCTTCCTGAGCCCTGTGCCAGCCTGTGCACATAGGATGCCAGCCTGTGCACATAGGATGCCAGCCTGTG CACATAGGATGCCAGCCTGTACACATAGGATGCCAGCCTGTGCACATAGGATGCCAGCCTGTGCACATAG GATGCCAGCCTGTGCACATAGGATGCCAGCCTGTGCACATAGGATGCCAGCCTGTGCACATAGGATGCCA AGGGAGGTGCTGCTTCTGAGGACTCTCGCAAGCTCCTCTTCCCCGTGACCTCCCTCAGCTGGTCCTGAGG GTCTAGGCGCCACAGAGGAAGAGAGAAGAAGCAGTGGCCCAAATGGCTCTGCTGGGGCCATGGAAGTCGG GATCATAAGGACAGGCGCCTAGCTTCCACATGACTGTCTGGCGTGCTTGAGAGTCAGCATGTGTCAGGCC AGTGCTTGTCTCCACGCTGAGATTCACTTTCGCTGGGGAAGGTCCATCCTTCCCTGTTCCACCAATTCAC ACCAGCTCTCTGCACGGCCACAGTGGCAGAAGAGGCAGGGCAGAAAGGAGCCTCATGCATCTAGAACTCT GGATTCTGCACAGTGGACAAGTTCCCCCACCATGCTGGCTTCCAGGATGGGTTCGCGTGACTCTATAGAG TGAAGAGGAACCACTGTTGCTCACCTCTACAGAAGGTTATAAGCCTCAATGCCCACTTCCCCTGAGCCCA CTGCCGAGGCCTAGACCTCCAACTCTGGTGTTCATTTAACACCATCTTGATTTTTGTTTCTGGCAAGATC TTACTAGTTAACCTAACTGGCCTGGAACTCCCTATGTAGCCCAGGCTAGCCTGGAAATTGCTATGTAGCC TAGGTTAGACTCAAAAATCATGATCTCCCTCAGCCTCCTCATTGCTGGGCTTCTAGGTGTTAGGCCCAGC TGAGCGCTTTGAGTTTGACTGCCTCCGTTCTGAGTCGGGCTCCTGCCAGAAGGCCCCCAGGACGTTCCCA GGCTGAGCTCTTGCCGCTCACCCATGCATTACCACTGAGACTCAGAAGCCTTCAGAAGCAATGACGTTAA TTGGGAGTATGTGCGGGGCTCTGCAGGGTTCCAGACTGGTGGTGGACCTTGTGTTCTGTGTGGCCCCCAC TGGCAGTCCCAGGGGTCTAGCCCAAGGAGACTGATGGATGCTGAGTCATGATCACATCACAAGCAAAGTT CTTAGCTTTTCTCCCACAACATACCCAGCTCTTTAAATTTAATGTCCTTTTTTTTTCTGACTTTTCTACC AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6850962 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6850962 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11976 same embryo
	EMAGE:11979 same embryo
	EMAGE:11977 same embryo
	EMAGE:11975 same embryo
	EurExpress:euxassay_010949 same experiment
	MGI:4828607 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source