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EMAGE:12013

Cdk19 cyclin-dependent kinase 19 ( MGI:1925584)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013
"Pseudo-wholemount" of euxassay_011117. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011117_01 euxassay_011117_02 euxassay_011117_03 euxassay_011117_04
EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013
euxassay_011117_05 euxassay_011117_06 euxassay_011117_07 euxassay_011117_08 euxassay_011117_09
EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013
euxassay_011117_10 euxassay_011117_11 euxassay_011117_12 euxassay_011117_13 euxassay_011117_14
EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013
euxassay_011117_15 euxassay_011117_16 euxassay_011117_17 euxassay_011117_18 euxassay_011117_19
EMAGE:12013 EMAGE:12013 EMAGE:12013 EMAGE:12013
euxassay_011117_20 euxassay_011117_21 euxassay_011117_22 euxassay_011117_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12013Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12013_wholemount_strong.wlz
12013_wholemount_moderate.wlz
12013_wholemount_weak.wlz
12013_wholemount_possible.wlz
12013_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12013_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37318
Entity Detected:Cdk19, cyclin-dependent kinase 19 ( MGI:1925584)
Sequence:sense strand is shown

>T37318
AGTCTGAAGAGCCTGTTTCCTGCACTAGGACCTCACAAGGAGTCACGCTGATCAAAGCACGCTTCTTCCA
CGTGAAGGAAAAGCTTTTCCCATGTGTGTCTTCAGACGCTTCTCTCAAGGGGCTGCTGGCTCTTCAGTCT
CTTCACATCTGGTCAGCATGAGTAACTTTCTTCCATAATCAAGGATACTCAAATAGAAGCTTGTTCATTC
ATCTGCAGGGTTTTCCATTTGTGTGTACATTAAGCATAAAGTGACTATTTTTAAAGCATGTTAAAATTTT
AGGTTTCATTCATGTTTGAAGTGTGTATTATGTATGCATAATTTTGCTGTTACTGAGACTTAACGCTGTC
AAGAATCTTTTGTATTGCACTGAATGCTTTCTTTTGCCCCTAGGACAAAAACCTTAATAATTGTGCCTAA
GAACTATGGGCAGATAGTGTAAAGTAGATGAGGTGAATATTTGCATTTCCATGATCTACGAATTAGAGGG
GAGTTCTATTTTAGCTACTTTTGAGGAACCCCCACCTCCAGAATCAAGAGGAAATGTAAAGGCTTAGAGC
TCCCAGTGTAGTGCAGGGGCAGAACACCTGTGTTCTGATGCTCCTAGCAGCACCAGCCTTGTTTGCAATG
TTTCTTGAGCTAGAAAAAAATAGCCAATTGTATATGCAAACTATATGCATTTTTAAAAAACTATTCTTGT
GAACCTATCTACCTGGTTATGATACTCTGGGGTCCATATACAAGTAAAATAAGATTTTTAGAAGCCAGTA
TACATTTTGCACTATTGATGTGATACTGTAGCCAGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101729. Forward Primer - name:101729_F_cDNA_2700084L06Rik, sequence:AGTCTGAAGAGCCTGTTTCCTG; Reverse Primer - name:101729_N_SP6_cDNA_2700084L06Rik, sequence:GGCTGGCTACAGTATCACATCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12012 same embryo
 EMAGE:12016 same embryo
 EMAGE:12015 same embryo
 EMAGE:12011 same embryo
 EMAGE:12014 same embryo
 EurExpress:euxassay_011117 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS