EMAGE mouse gene expression spatial database

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EMAGE:12097

C130026L21Rik RIKEN cDNA C130026L21 gene ( MGI:3041189)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008424. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008424_01	euxassay_008424_02	euxassay_008424_03	euxassay_008424_04

euxassay_008424_05	euxassay_008424_06	euxassay_008424_07	euxassay_008424_08	euxassay_008424_09

euxassay_008424_10	euxassay_008424_11	euxassay_008424_12	euxassay_008424_13	euxassay_008424_14

euxassay_008424_15	euxassay_008424_16	euxassay_008424_17	euxassay_008424_18	euxassay_008424_19

euxassay_008424_20	euxassay_008424_21	euxassay_008424_22	euxassay_008424_23	euxassay_008424_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12097_wholemount_strong.wlz

12097_wholemount_moderate.wlz

12097_wholemount_weak.wlz

12097_wholemount_possible.wlz

12097_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12097_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9463
Entity Detected:	C130026L21Rik, RIKEN cDNA C130026L21 gene ( MGI:3041189)
Sequence:	sense strand is shown >T9463 ATCCGAGAAGTCCCTGATGCTGAGGTCTCTGCTGACTTGCCCCGAAGGATTGCCGACACACGGACATTTA TGTCTTCCCCACAGGGAAAAACACCCCATTAGAAGGCAGAAATTTGGTTCCCCCATAGGATCTTCAGGGA GCTGCAGTGACACCAGAGGAGGGCTACTGGAAATGGCACCAGGACATCATATTCGTTTTCTAGATCCCAA CATGGAAGAATTGGAAAGAGAAAAGCATTATATGCTCATGGATGCTAGTGGAGTTTGTTTCCAGCCTGTC GTGTTTCCGGGCCTTGGCTTAGAGACAGAGGTGGCTTCCTTCCTTCCCTGGGGGAGGCTTCATTCCTGAC TCCTGACCTGCCTCTTCTCCTGCCCACATCCTTCCTTTCTGAGTGATCCTGACTGGCAAGAAGGGACAGC AGTGAGGAGCCTCTCAGAACGGAGACCATGGCTCCTGCTTCTCAGCCTGGCTAGCCCACCTGCAGGAAAC CTGATTGTACCCTCATGGGGCCATCCTTTCTCTGTCCCCAAAGTACCCAATACACCTGGGCTTTCTTCAG GATCCCACTCTTTCCCTCCTCCCTCCTCCCCTCCTCCCCTCTTTTCTTTCTTCCTCCATCTCTGCCTTTC TCCTTTCTGCCATACACTGCCTGACCCATCAGATGTCATTGTTTGCTGTTTAGACTGCAGTAGCCACTGA AAGCAGACTCTGCAGGTTGAAGCTTGACCTCCATCTCTGCTGTACACGGCTTTGGGAAGTTCCTCCGGCG CTCCAGGCTTTCATTTCATCGCCTTGAATGAAGGTTCTTCTGGGGATGAAATAAAGTAGTCATTGGTCGT TGCTCTGTCACGACTGATAGATGAGTAAATCCCAGAGACTTCAGAAACAGAAGCCAGAGGAAACGGCCAC CCTGCTGAGCCCTGGAGCTGCTGGCAGGCAGAGCCCTCTCTCTAAGTCACTACCAGACAGGCTGGCAGGA AAATCTGGAAGAATACAGATTTGATGACAGGGATGTCCTAGGAGGGATGTCTCCAAGTCCAACCTCTGCC CTTGGACCTCTCAGGTGCAAAGAAAGCGCAGGTTCTCAGAGCTGTGGTACCCAGCCCTCTGTGATCCCCA CCATCAGCTGGATCCAGAAGGGCTCCTCTCTTCATAGGGAGCCTGAGGCATTCATTGCTCTCACTAAGGG CCCAGAAAAGGCAAGCTTTGGTGCTGAGAGCCCCCTAAGCTCCCCACAAACACTGTGATTGCTAAAAATT TGCTTCGTTCTGATTTTGCCTCTCGTTGGCTGAAGGACTGGATTTTATACCAGGTCAATTTCAGACCATT TGATATTGATATATGAAGATCCCAGGGGATTACTGAATGCATTTCTCATCCTGCCTAGATAGTATCATAG AAGGAAAATGGGATTGACTGTTAAGGACAAATTGAGAAAGACATGGCCATTTAAGATAACTCCTTATCTG TATACAAGCAAATGGTATCTGGGAAATAGACCACCAGTTGACAGCAAGAAATTCTACTCCGGGCTCTTCA CCCACTCTCCCTTCTGCCGTTAACACTCAGGGATTATCCCGTTATCCTGAGAGCCATTTCCTGGGGGCCC CTGCTGGAGGACACTAGAGAAGAGGGCTTTTTGAGACTTTATTCTGAAAGGGTTGGGTGGAAGGGGTCTG GGATTGTCCATGGGTACTCTGAGGAGTTTGGGATGGAATGGCTATGAATGTGACAGATCTCCCTGGTAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30359051 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30359051 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12095 same embryo
	EMAGE:12099 same embryo
	EMAGE:12096 same embryo
	EMAGE:12098 same embryo
	EurExpress:euxassay_008424 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source