EMAGE mouse gene expression spatial database

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EMAGE:12098

Cldn2 claudin 2 ( MGI:1276110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008493. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008493_01	euxassay_008493_02	euxassay_008493_03	euxassay_008493_04

euxassay_008493_05	euxassay_008493_06	euxassay_008493_07	euxassay_008493_08	euxassay_008493_09

euxassay_008493_10	euxassay_008493_11	euxassay_008493_12	euxassay_008493_13	euxassay_008493_14

euxassay_008493_15	euxassay_008493_16	euxassay_008493_17	euxassay_008493_18	euxassay_008493_19

euxassay_008493_20	euxassay_008493_21	euxassay_008493_22	euxassay_008493_23	euxassay_008493_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12098_wholemount_strong.wlz

12098_wholemount_moderate.wlz

12098_wholemount_weak.wlz

12098_wholemount_possible.wlz

12098_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12098_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 moderate expression: see section 11 12 14 15 16

diencephalon roof plate

moderate

regional

moderate expression: see section 14 15

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 10 11 12 16 17 18 19 20 21

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22 23

midgut

moderate

regional

moderate expression: see section 09 10 13 14 15 16 17 18 19 20 21 weak expression: see section 08 11 12

kidney calyx

strong

regional

strong expression: see section 06 07 08 09 10 21 22 moderate expression: see section 11 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8399
Entity Detected:	Cldn2, claudin 2 ( MGI:1276110)
Sequence:	sense strand is shown >T8399 AGGGTTTCATTCCCATCTGTAAGAAGGCTCAGAGTTACAGGTGGAGTTTACAAAGCTGTGGCTAATAATT AGAAATCCCTTATCACCACAGCTTGTGACCCCTTGGACCCCGTTCGCCTTTCTCTGGACCTAGTCCTTGT CCCCAGAAGCGTCTCTTCCAGAGCTCTTCGAAAGGACGGCTCCGTTTTCTAGATGCCTTCTTGAGCCTGC TTGTGGCCACCCACAGATACTTGTAAGGAGGAAAGAAGTCAGCTTGCCAGAGACACTGTGAAATAAGGGA TTAGAGGCTCGGATCCAAGAAGCAAGAGCAGCAGCCAAACGACAAGCAAACAGGCTCCGAAGATACTTCT ACTGAGAGGTCTGCCATGGCCTCCCTTGGCGTCCAACTGGTGGGCTACATCCTAGGCCTTTTGGGGCTGT TAGGCACATCCATTGCCATGCTGCTTCCCAACTGGCGAACGAGTTCCTATGTTGGTGCCAGCATTGTGAC GGCGGTTGGCTTTTCCAAGGGCCTCTGGATGGAGTGTGCGACACACAGCACAGGCATCACCCAGTGCGAT ATCTACAGTACCCTTTTAGGACTTCCTGCTGACATCCAGGCTGCCCAGGCCATGATGGTGACGTCCAGTG CAATGTCCTCGCTGGCTTGTATTATCTCTGTGGTGGGCATGAGATGCACCGTGTTCTGCCAGGATTCTCG AGCTAAGGACAGAGTGGCTGTAGTGGGTGGAGTCTTTTTCATCCTTGGTGGCATCCTGGGCTTTATCCCA GTTGCTTGGAATCTTCATGGCATCCTTCGGGACTTCTACTCGCCGCTGGTTCCTGACAGCATGAAATTTG AGATTGGAGAGGCTCTGTACTTGGGCATCATCTCAGCCCTGTTTTCTTTGGTAGCCGGAGTCATCCTTTG CTTTTCCTGCTCGCCCCAGGGCAATCGTACCAACTACTATGATGGCTACCAGGCCCAGCCTCTTGCCACT AGGAGCTCTCCAAGATCTGCTCAACAGCCCAAAGCCAAGAGTGAGTTCAACTCATACAGCCTGACTGGGT ATGTGTGAAGAACCAGGGGCCAGAGCTGGGGGTAGGGTGGGGTAGTGGCTGGGACTATATATATAAAAAA AAGCAGTGGACAATGCCCCCAGGGTCACAAGCAAAGGACATTGCCACTGAATCATGTGTAAGGTGCTGCT GAGGGTAGAATGACTTTGGCCATTGGATGGAGTGAAGGCAGAGATGAGAAGAGGGGTGTCAGCATGTAGA TGGCATTGCCAAGGACGCTTGCCAAGCTAGCTTTTCTATTTCCCTCACCTGACCTCACTAAAGTACCTCC TGTGCTTTGAATGCTCAACCCAAAAGCCCAAATCCCATCTCTGCCACTTTAGGCTATGCCCAGAGGCTCT CTTCTGCCCTTTGGTTTACTTGGAGATCTGTCCCCAAACCAATCATATCCTGTTGACTAATCACCTGTGA TCAAAAGACCCTCTCCCTCTGGCTGAGGTTGGCTCTTAACTCAATTACTAAGGGACAGAGAGAAGAAAGA GTGGTGGCTTCTGTGAGGACATTTCTAGCTCCCTTCTCAAATCTCCAGCCAAAGAAATGGATTAGTCAGT AATGGGCCCTGAAGCTTCTCTTCCAGTTTTGCTATGAATGCAGTATGTCCAGACTGCATTGTGAATGAAC TGAAGGAAAGCTATCTCATGGCATCCAGCAGAATACAGCTGGGTGCAGGATGGGAGGCAACCTGGGATCT AAAGAAACAAAACAAACAGCAAAACCCGACAGAATCCCTTCTCAGGCCATCTCCTATAATTCCCGTACAT TTGTGGGTCAGGGATGGAAGCACTGCTAAGGAAAGCACAAGAAGCCAAGGGAAAGCACATGGCTGCTGCC CTCTGACTCCACAGAGGAATTGCCCAGAAGCCAAGGTGAACACGGACCACTGAAAGCTCTGCCTCCCACT GGGGTGTCTGACCCAGCTTCCTGCTAAACCACAAGACTTGGAGCCCTAGACAAGGGTTTCCTTAAGGACA ATAAACCAGTTTTTCTAGAACTGCCCTTTGACATGCGAGTTGCAGAAGGCTTAAAAAAAATACTACCCTT TAGCCCTGACCGAGAAAGAATAAGGGCTTGGGTATCAAAGAACCGGACAGAGATGTGTGGTAGCTGTTAT GTGTGCTCCTACTGTTTCAGAGAGGCAGCCCTACCAGATACTTTAGACTTGATGGAAGACCAACCCATCA GGGTCTATGTCTCAGCACTACAGAGCCTCTGAAAGACCACAGCACCAGAGAGGTCCTTTGTCACTTTTCA CACTTGAGTCATCGCCCATCAGAAGATACTCTCACAGGTATGCAAAGTCTCCAGTGTGGAACATCTGTGA CCAAGGTGTTGTGTTCATGACCTGAGCAATTGGTGAACTTCTGCCTGGGATTGTGCTTGAGGTGGGAGAA CGGAGACTGGTTCCCTGTCCAACAGTTTTATTTCCACGGTCTTAAAATGCTCCATTTCTTCTGTGGTAGA TGGGGGTCATAAACCAGAGACATTTCCCCACCACCACCAGCTTAATATCACCAGCTTCAGAAGATCCCCA GCGCACCTCATCAAAACTTCAGCACCGGCAAGAGAGGCCCATATGTGAGGTTTCCCTTAACATGCCTTTC CTATTTCCACCATATTACTTGTGTTCAAGCTGATATGCTGACCTGGAAGCCATTTCCTTTTAACTAAGTC ATCCCCATGCTTGGAGAACTTGCTCATTGAGCCTTCTCTGACCACTCCTTCCTGTTCTTTCCTTTCCCTC CTCCAACTCCCAATATATAAATTGCCTAATCCTCATCATTCATAATTTTGATTGATAGTTTATAGTTCTT GTATGTGCATTTGTGTGTGACTCTATCCGTCTGTCTGTCTGTGTGATCTCAAAAGTTCACTGTAAGTACT TGGAGGGCAATTGCTATATCTTAGACCTGTCCATATGTATACCCAAGACTGTAACAGTGCTGGGCACACA CAAGGTGATCAATAAACGGATGTTGATTGAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686202 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686202 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12095 same embryo
	EMAGE:12097 same embryo
	EMAGE:12099 same embryo
	EMAGE:12096 same embryo
	EurExpress:euxassay_008493 same experiment
	MGI:4823909 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source