EMAGE mouse gene expression spatial database

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EMAGE:12168

Zfp811 zinc finger protein 811 ( MGI:2682944)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007048. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007048_01	euxassay_007048_02	euxassay_007048_03	euxassay_007048_04

euxassay_007048_05	euxassay_007048_06	euxassay_007048_07	euxassay_007048_08	euxassay_007048_09

euxassay_007048_10	euxassay_007048_11	euxassay_007048_12	euxassay_007048_13	euxassay_007048_14

euxassay_007048_15	euxassay_007048_16	euxassay_007048_17	euxassay_007048_18	euxassay_007048_19

euxassay_007048_20	euxassay_007048_21	euxassay_007048_22	euxassay_007048_23	euxassay_007048_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12168_wholemount_strong.wlz

12168_wholemount_moderate.wlz

12168_wholemount_weak.wlz

12168_wholemount_possible.wlz

12168_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12168_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 21 moderate expression: see section 06 07 08 09 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 21 moderate expression: see section 08 09 22

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 21 moderate expression: see section 06 07 08 09 10 19 20 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 09 10

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 21 moderate expression: see section 08 09 10 19 20 22

trigeminal v nerve

moderate

regional

moderate expression: see section 11 19

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

cervico-thoracic ganglion

strong

regional

strong expression: see section 13 14 18 19

cervical ganglion

strong

regional

strong expression: see section 11 19

thoracic ganglion

strong

regional

strong expression: see section 15 16 17

neural retina

strong

regional

strong expression: see section 01 02 03 04 05

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

rectum

strong

regional

strong expression: see section 15

midgut

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8390
Entity Detected:	Zfp811, zinc finger protein 811 ( MGI:2682944)
Sequence:	sense strand is shown >T8390 TCCATTTCTGCCTCACCTCCACTGACTGCAGGAACTACAAGAGGACACTAGGACATCATGTCTCTGGAAA TGGAGCTGGTGACCTTCGAGGATGTGGCTGTGCACTTCACCATGGAGGAGTGGGCTCTGCTGGATCTATC CCAAAAGGAGCTCTACAAAGACGTGATGAAGGAGATATTGAGAAATGTGGTTTCCATAGGAGACATAAGG AAACACCAAAAGATTGACAATAAATACCAAAATTCTGGGAGAAAACTAAGATGTCAACTTGTGGAGAAAC TCCATGACTGTAAAGAAGGTCATGAGTGTGCAGAGATCTTCAAATTCTCTACAGAGGGCACTGTGAACCA TGAATTTTATCCAGGAGTACATATATGTAAAAGAAATGGGTTTGTCAATGTACTCATTGGCCATTTAGCT ATAAATGTGCGACTGAAACCTAGGCCAGGGCAGAAGTCAAATGAAGTCCAGGAACATGGAAAGAATGTGT ATACTAAGGAATCTCAGGGCACCTCCAGCGCTTCCCCGTCTCTTCAGAAGCATCAAAGTGCTGAGCCTGC AGAGAAATCTGATAAAACTAAACAGCCTGATGAAGACTTGAGACTTGGTCAGAATCATGAAAGGACTCCT GCTGAAGAAAAACACCACAAATGTAAACACTGTGGGAAAGCTTGTACTTCTCTCAGCTCCTTGAAGAGGC ATGAACAACATTACCATGCAAAGAAATGCTCTAAGTGTAAGCAGTGTGGGAAAGCCTTCCCTTTCCCGAG TTCCCTACAAGTCCACCAAAGGATCCACACTGGAGAAAAGCCCTATGCGTGTAAGACGTGTGGGAAGACC TTTGGACATTCCAGTTCTCTCCTAAGACATGAAAGAATCCACACTGGCTCGAGTCCCCATGAGTGTAAAC TGTGCAAGAAAACCTTTACTCGTTTGAATTCGCTTTTAAGGCACAAAATAATTCATACTGGTGAGAAGCC CTATGCATGTAATCAGTGTGGGAAAGCTTTCTCTCGATCCGCTTCCCTTCAGAGACACACAAGACTCCAC ACTGGAGAGAAACCCTATGTGTGTAAGCAGTGTGGGAAAGCCTTCATTACCTCTACTTACCTACAAGTTC ATGAACGAACTCACACTGGGGAAAAACCATATGTATGTAAGGAGTGTGGGAGCACCTTCATGCTTTGGAC TCAATTCCAAAAGCATAAAATAGTTCACAGCGGTGTGAATCCCTTTGTTTGTAAGCAATGCGGGAAGGCT TTCACTCGTCTTGGTACCCTACAGATACATGAGAGAATTCACACTGGTGAGAAGCCCTATGTGTGTAAGC AGTGTGCAAAAGCCTTCATGTCTTTGACTCAGTTAAGACGACATGAAATAATCCACAGTGGCATCAAACC CTACATGTGCAAACAATGTGGAAAGGGGTTTCTCTGCTCTACTACTCTTCGGAGTCATGAGAAGATCCAC AGTGGCAGTAACCCGTATGTATGTTCTCAATGTGAGAAAGTTGTGAGTTCTGAGAGCTCTTTGCGGAAAC ATAAAATGATTCACACTGGAGAGAAGCCTTGTGTCTGTAAGCAATGTGGCAAAACATTCACTCATTTAAG TTCTCTTCAGTACCATGCACTGATGCACAGCGGAGAGAAACCCCATGAATGTAAGAAATGCGGGAAAGGC TTTCGGTCTCTTAGTCACCTTAAAAAGCACGAGAGAACTCAACACTAAATAGAAACTATATGCATGGAAG CAATATGGGAAAGCCATACACAGTGGCATAAATCCATAGGCTTGTTTGTAAGCAGTGTTCACAGCCTTTA AATAGCTGTGGTGTTTTCACATAAACTCACTGGAGAAAAGGGGGACTTTTTGCTAACAATATGTGAAAGT TCCCTATAGGATTGATTTTAAAAAACAAAAACCCATGAAATAGCCAGAGTGGAGGGGGAACCTTTTTAAA AAATAATATGGAAGCCAGGCAGTGGTGGTGCATGCCTTTAGTCCCAGTACTTGGGAGGCAGAGGCAGGTG AATCCCTGTGAGTTCAAGACCAGCCTGGTCTACAGGGGGAGTTTTAAGACAGCTGAGGCTGTCCTGTCTC AAAACAGAGAAACCGTGTCTCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5685048 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5685048 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12172 same embryo
	EMAGE:12171 same embryo
	EMAGE:12170 same embryo
	EMAGE:12169 same embryo
	EurExpress:euxassay_007048 same experiment
	MGI:4829342 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source