EMAGE mouse gene expression spatial database

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EMAGE:12171

Morn4 MORN repeat containing 4 ( MGI:2449568)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007046. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007046_01	euxassay_007046_02	euxassay_007046_03	euxassay_007046_04

euxassay_007046_05	euxassay_007046_06	euxassay_007046_07	euxassay_007046_08	euxassay_007046_09

euxassay_007046_10	euxassay_007046_11	euxassay_007046_12	euxassay_007046_13	euxassay_007046_14

euxassay_007046_15	euxassay_007046_16	euxassay_007046_17	euxassay_007046_18	euxassay_007046_19

euxassay_007046_20	euxassay_007046_21	euxassay_007046_22	euxassay_007046_23	euxassay_007046_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12171_wholemount_strong.wlz

12171_wholemount_moderate.wlz

12171_wholemount_weak.wlz

12171_wholemount_possible.wlz

12171_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12171_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 10 19 20 21

trigeminal v nerve

strong

regional

strong expression: see section 09 10 11 18 19

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 12 13 14 18 19

cervical ganglion

strong

regional

strong expression: see section 10 11 18 19 20

thoracic ganglion

strong

regional

strong expression: see section 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 03 04 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 09

tongue

strong

spotted

strong expression: see section 13 16 17

stomach

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 11

midgut

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8394
Entity Detected:	Morn4, MORN repeat containing 4 ( MGI:2449568)
Sequence:	sense strand is shown >T8394 GTTGTTGTTTCCCTCTTTTTGCTGCTTGCTTTTGTTCTATTATTTTTCCTATTTATTTATTTTGAGGCCT TTGCGTTTAGCTGAGGATGACGTCGAATGCATGAGCCGCCTGACTCTGCCGAGGCTGCCGACATGCACCA TGGTTGTGCTGCGGGCTGCTCACCTGTGTACTTACTAATTTCTGCCATCTGAGCTGATGCAGAATTACCT TGTCTCTGGTATAGCGTGTGGTGCTTCACCTGCTGAGCCTACTGACCATATGATAACTTCATTGAGGGGA CCCTGTTTATACATCCACTGAGCACACAGTACGTGTCAAGTAAATATTGGTAGATTGAGCTTTCCTCTTC ATCACAGGAAACAGGAACTGGCTGAAAACTATTAAAGAAGTAGGGGTGAGGGTGCCATAGCAGAGGCGAA GGTGGTGGCGAGGAGGGCGTTTTAAGTGACCTGAGGTAGGAGTCCACTTGAGGTAAGTGTGCACTTGCCG GAGCACCGCCTCTAGTCCACTAGCCATTCCGTGACACTGCTTTCTCTCTCTGTTTCTTAGAGCTGTCATC CTCTTCTTCTGTAAGCTGCCACCATGACCCTGACCAAAGGTTCCTTTACCTACTCCAGCGGGGAGGAGTA CCGCGGCGAGTGGAAAGAGGGCCGGAGACATGGCTTTGGTCAATTGGTGTTTGCAGACGGTGGCACCTAC CTGGGTCACTTCGAGAATGGGCTTTTTAATGGCTTTGGAGTCCTGACCTTCTCAGATGGCTCAAGGTATG AGGGGGAGTTTTCCCAGGGCAAGTTCAATGGCGTCGGAGTCTTCATTCGATATGACAACATGACCTTTGA GGGGGAATTTAAAAATGGCAGAGTAGACGGTTTTGGCCTGCTGACTTTCCCAGATGGTTCTCATGGAATA CCCCGCAACGAAGGTCTGTTCGAGAACAACAAGCTACTGCGGCGTGAGAAGTGTTCCGCCGTGGTTCAGC GGGCTCAGAGCGCCTCCAAGTCAGCCAGGAACCTCACTGCCTGACGGAGCTTTGGGCGCAGCTGGAAAGG ATCCGTAGAGCAGTGGCTTCCCTTGTATGCGTGCAGGTGAACAAATGAAACGGATATGAGATGAGAATGA CCTTGATGCCACAGCCTGGGAGGTGCTCCGTCCCCTGCCGACCAGGAGTTCAGTCGCAGAAAAGTGTGGA GGGGTTTGGCCCCAAGGCCCTGCGGCAGTTATGTCCTTCCCTGGCCCCGATGTGCTCAAGAACAGGGCCT GCCCTTTCTTCCTTGTTATCCCCAAGACCTTGGGTTACCCAGTGCTGTGGCCTTCTCTCCGCCTGCTGTC AAACCATACAGACTCTCTTCATTCTGCCCACCCTTACTTACCGCAGATAATCTGGGCCTAGACTCAGAAC CAGTCCCGTGTGCTACCATTGGAGACATCAGGAAAAAGACAGGGGTGACCTAGACCACAGCAGCTCTGTG AAGTCCAGGCCAGAGCTCTTCTGGGGGTTACCCACTCATTCACTGCAATTGGAGGCCTGGCTCCAGACTG GCCTCTGTGTCTTCTTATCCCTCCTCGGTGTAAGAGTGTGGTTTTCTTCTCCCTCAGCTTTAGCTGCGTC TTCCTTACCTGAGTTACAGCAGACAGCCCAATCCCCGTCGGTGCCTCTCTCCCCCCTTAGACATCAGACA GGATCCCTTTATAGAATAGCCAAGTAAGAAACCAGTGTAAAAGTAACTTATGACCTGCACTCCTGACTCA CTCCAGGGTGAAGGGCAGACCTATCCAGGCTTTCTGTGGAATGGCCTGGAATCCCAGGAAAGGCCTGCAC TGAGAGCTCCTTCCCAGCGTACCATTGGATCAGGCTCACTGTCCCCTGGGTGTTCACAGACTTGGGTCCA TCCAAGCGTGAGAACTGTCTAGCATGGGCTTCTCCTTTTGACTCTGGAAGCAAGAGTCTTGGCCCTCAGG CCTGGGACAGATCTTCCTGCCAAAATGATCATAAGGTCTCCAGTTAGCCTAGTCCCAGTCCCAGCCTAGT CCCAGCCCCAGCCCTAGTCCCAGCCCCAGGTCCCATCCTCCAATCTCTCTCAACAGCAGACTGTGGTGGA AATTATAGCTGTGTGCCTCTATTTATTATCTGAACTGTACTGTAGAAACAAGTGTTTATAGGAATAAACC AGTCACCAGAACTGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5685582 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5685582 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12172 same embryo
	EMAGE:12168 same embryo
	EMAGE:12170 same embryo
	EMAGE:12169 same embryo
	EurExpress:euxassay_007046 same experiment
	MGI:4826422 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source