EMAGE mouse gene expression spatial database

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EMAGE:12197

Ubxn4 UBX domain protein 4 ( MGI:1915062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008244. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008244_01	euxassay_008244_02	euxassay_008244_03	euxassay_008244_04

euxassay_008244_05	euxassay_008244_06	euxassay_008244_07	euxassay_008244_08	euxassay_008244_09

euxassay_008244_10	euxassay_008244_11	euxassay_008244_12	euxassay_008244_13	euxassay_008244_14

euxassay_008244_15	euxassay_008244_16	euxassay_008244_17	euxassay_008244_18	euxassay_008244_19

euxassay_008244_20	euxassay_008244_21	euxassay_008244_22	euxassay_008244_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12197_wholemount_strong.wlz

12197_wholemount_moderate.wlz

12197_wholemount_weak.wlz

12197_wholemount_possible.wlz

12197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7215
Entity Detected:	Ubxn4, UBX domain protein 4 ( MGI:1915062)
Sequence:	sense strand is shown >T7215 CGGAACCCGAGGCTTCGCGGGGGCGCGCACTTACGGACTTACCTAGCCGACCAGGCACCGGGGCGCGCGA CCCCTGACGAGGAGTGTCGTCGTCACGTGACACAGGGGCGTACTTTTCTTGCCAAGCGCGCTCCGGTGCG CGTCCCCAGGGGGTGGCTGTCGGGCGTACGGGCTGGCTGGGTCCTCAGACCGGGGCCCGGATTAGCCGGT GGTTGCCGGGGCCACGAGGCCAAAGAACCAGGAGGGCGGGGTCGGCTGTGGCACCGCGGCGACGACACAC CGAGCGAGCGCCTGGGCCTGAAGGGGACGATGCTGTGGTTCCAGGGCGCCATCCCGGCGGCCATCGCGTC TGCTAAGAGGAGCGGCGCGGTCTTCGTGGTGTTCGTGGCAGGGGATGATGAGCAATCTATACAAATGGCT GCAAGTTGGGAAGATGAAAAAGTGACACAAGCATCATCAAACAACTTTGTTGCTATTAAAATTGATACTA AAAGTGAAGCCTGTCTGCAGTTTTCACAAATTTATCCTGTAGTATGTGTTCCATCCAGTTTCTTTATAGG AGACAGTGGAATCCCTTTGGAAGTAATAGCGGGGAGTGTTTCTGCAGATGAGCTTGTTACAAGAATTCAT AAAGTCCAACAGATGCACTCGTCAAAAGGTGAAGCATCAGTGACAAATGACAACCAGTCAGAAAGTTCAG TATCTACCCCATCTGCCTCATTTGAACCTGATGTATGTGAAAATCCCGAGTCCAAAAATACAGAGCTTTG TGAGACACCAGCTACCTCTGACATAAAGTCAGATACTGCAACAGGAGGAGAATGCACAGGTCATGACAGC CACTCTCAGGAGCCTCATGGATGTTCAAACCAGAGACCAGCCGAGGACCTCACCGTCAGAGTAGAAAGAC TAACAAAAAAACTTGAAGAAAGGAGAGAAGAGAAGCGGAAGGAAGAAGCACAGAGAGAGATTAAGAAGGA AATTGAAAGGAGAAAAACTGGAAAGGAAATGTTGGATTATAAAAGAAAACAAGAAGAAGAATTAACAAAA AGAATGTTAGAAGAAAGAAGCAGAGAGAAGGCAGAAGATAGAGCGGCTCGAGAGCGCATCAAGCAGCAGA TTGCCCTGGATCGTGCAGAAAGAGCTGCTCGTTTTGCAAAGACAAAGGAAGCAGAAGCTGCCAAAGCTGC TGCCTTGCTGACCAAACAGGCAGGAACGGAAGTCAAGAGAGAGTCTACAGCACGAGACAGAAGCACCATT GCAAGAATTCAGTTCCGGCTGCCTGATGGTTCTTCCTTTACAAACCAGTTTCCTTCTGATGCTCCTCTAG AAGAAGCGAGGCAGTTTGCTGCACAGACCGTGGGCAACACCTATGGCAATTTTTCATTAGCCACCATGTT TCCCAGAAGAGAGTTCACCAGAGAAGACTATAAGAGGAGGCTGCTGGATCTGGAGCTCGCCCCCAGCGCC TCTGTGGTGCTGCTGCCAGCAGGACGGCCAGCCACATCCATTGTCCACTCTTCTAGTGGAGACATTTGGA CATTACTGGGGACGGTGCTTTATCCATTCCTGGCCATATGGAGATTAATTAGCAATTTTTTATTTAGCAA TCCTCCTCCTGCACAGACTTCAGCAAGAGCCACATCCACAGAGCCTTCAAACTCTGCATCATCCAGCAAA TCAGAGAAAAGGGAACCAGTCCGAAAAAGAATGCTGGAAAAACGAGGGGAAGACTTTAAAAAAGAAGGGA AAATATACAGACTGAGGACTCAAGATGACGGTGAAGACGAGAACAACACTTGGAATGGAAACTCCACGCA GCAGATGTAGGGTAGCGAGCAGTGCAGATTCTCACTGTTTCCTTAGGACTCCACTCAGCGAGACTGCTGC TGGAGAAGTGGGTCAGCTTTATGTTTTCAGACCGTGTATAGTCTTTATTTCTGCTAAGAGGTTTTGAGTT AAGTTGTTTAACCTGAAAAAGTCAAACATGAAATAGCTGGATGCTAGGTCCTTGTGTAGGGTAACCAGCT GTTGAGCCTAGAAGAATCCCAAGGCCACCCTGCCACCACCGCTCCTACTCAGACTCCACAGCCCCTAGCC CAGTGTTTCATTTAGGATACTTTTTGCTGCAAGATTCTCAAGCCACACACAGTAACTGGTATGTCACTGA GTAGTCTGAGCCCCGAGCACCTCAGCGAGCTAATCAGGTGATAACTGATGTTTAGCGTTTCTCTTTGCAC TCATCAGCCTCAAAAACAAAACAAAAAAAAAAAAATCTTGTAGTTTTTGATCTTACTTAAATATCAAATA AAAAAAGTTTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4158848 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4158848 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12201 same embryo
	EMAGE:12199 same embryo
	EMAGE:12202 same embryo
	EMAGE:12198 same embryo
	EMAGE:12200 same embryo
	EurExpress:euxassay_008244 same experiment
	MGI:4829068 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source