EMAGE mouse gene expression spatial database

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EMAGE:12201

Mfsd9 major facilitator superfamily domain containing 9 ( MGI:2443548)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007136. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007136_01	euxassay_007136_02	euxassay_007136_03	euxassay_007136_04

euxassay_007136_05	euxassay_007136_06	euxassay_007136_07	euxassay_007136_08	euxassay_007136_09

euxassay_007136_10	euxassay_007136_11	euxassay_007136_12	euxassay_007136_13	euxassay_007136_14

euxassay_007136_15	euxassay_007136_16	euxassay_007136_17	euxassay_007136_18	euxassay_007136_19

euxassay_007136_20	euxassay_007136_21	euxassay_007136_22	euxassay_007136_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12201_wholemount_strong.wlz

12201_wholemount_moderate.wlz

12201_wholemount_weak.wlz

12201_wholemount_possible.wlz

12201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7177
Entity Detected:	Mfsd9, major facilitator superfamily domain containing 9 ( MGI:2443548)
Sequence:	sense strand is shown >T7177 GTCTGCAGTCGGCTAAGCTGTCCGCACCCCCAGCCCTCCTGGGAATGGCGCTGGGGGTCCCGGGGCACGG GGCTTCGAGGCAGGATCTAGCCTCGGAGACCCCGGTGACAGCCACAGCACCGTCTAGTGCTGTGAGCTCC CGCCGCTTCCTGCTCTGGCTCTACCTTGTAGGCTTCTTGGACCTGTTTGGTGTCAGCATGGTGGTCCCAT TGCTGAATCTTCATGTCAGATCCCTTGGAGCAAGCCCCGTAGTGGCTGGAATAGTAGGCTCCTCATACGG CGTTTTGCAACTCTTCTCCAGCACCTTTGTGGGCTGCTGGAGTGATGTGGTCGGAAGACGGTCCTCCTTG CTGGTGTGCATTCTGCTTAGTGCCCTGGGCTATCTCCTCCTTGGAATGTCCACCAATGTGTTCCTGTTTA CGCTGGCTAGAGTCCCCGTGGGTGTTTTTAAACACACCCTCTCCATCTCCAGAGCACTGCTTTCTGACCT GGTCACAGAGAAGGAGCGACCACTTGTCCTGGGACAGTTCAACACAGCCTCAGGCGTAGGCTTCATCTTG GGCCCTGTGGTTGGTGGTTATCTGACTGAACTGGATGGTGGATTCTATGTGACAGCCTTCATTTGCTGCT CTGTCTTCCTCCTCAATGCTGGTCTAGTCTGGCTGTTACCTTGGAGAGAAACAAGCCTCAACATCCCAGA CAATGGTTGGAGCTTGGATGGGAAGCACACCACAGAACCCATAAAGTCAGAAGGAACACTACAGGGAGCA GCCACCACCTCTACGGCCAGGAGGAGTGAGAGGACAGTGCAGGCACCCTGGGTTGAAGTCATGTTGGCCT TGAAAGACATGAAGAACCTGATCTTTTCTGCTCTGTGGGATGTATTCCTCATGCGTTTGCTGATGGGCGT GGCCGTCATGCTGTACTACAGTAACTTTGTCTTGGCCCTGGAGGAGCGGTTTGAGGTACGGCCCAAGACC ACAGGGTACCTCATCAGCTACACCAGTGCGCTGGGCACCCTGGCTGGCTTTGCTGTGGGACCCATTCTAC GGCTGTACAAGCACAACTCCTACATGGTCCTCCTGCACTCCAGCGTTCTCACCTGTCTGCTGCTGGTGGT CTACTCCACGACCTGCTCCATGGCCATGGTGGTCTTCTCCTCGACGCTTCTGTCCTTCTCCACCACCATT GGGAGAACCTGCATCACGGACCTCCAGCTGAGTGTGGGTGGAGCCCAGGCCAGCGGCACCGTCATAGGTG TGGGCCAGTCCGTGACTGCGGTGGGCCGCATCCTTGCCCCTCTTCTCTCTGGAGTGGCTCAGGAGATCAG TCCCTGTGGACCCCCAAGCCTGGGTGCTGCTTTAGCCCTGGTAGCTATTTTAATCATGACTCTAAACAGA CCCCCCTTCAGTGGGGATGGGAGTGAGAGACTCAAACAGGAGTAGGATTGGGACCACTTGGAGAAGCAGA TGTAGAACCAGGTAACTCCGGGTCAAAGATGGGCACTTGAGAGACAGTGGGTGGTCCAAGTGTAGTTATT CCCACATGGCTGAGCCTGGTCCTTGTTTGCACAGGCATGCCAGCAGACCCATCAGAAGTCAGCCGCAGAT AGGGAAGTTTAGACTGAGCATAGCGCTGCCCTGTGTCAGAAGCTTGCCTGAGAGGCTTCTCTTCCCACCT GGGCACAGTAGAGTTAACATTTGCTCTTAGGGAAAGGCAGCATGCTAAAAGAGGTAGGAGAAGGAATGTG GAGAGAAGCCTTTGTGTTAATCATGGACTGGTCACCTATCTGGAATGCACCTCCCCATAATACCAGAAGC CAAGAAGTTTCTCTGCCCAAATCCCAGAGACCAACCGATGCATCTGCTTTATTTAAACCCTGGTAACATC AAATGCTGAGCCCTTATCCCCTGACATTCAGATACACAGACCTCGCTGAATTCAGACCCGTGGGTTTTAA AGCCCCTGTAGCCATTTCTCATGGAGGGGAGAGGAACACTGATACATGATAGTTTTGATTAAAGTAAAAA TTGCAGACTACACTGTTTTTACTAGTTAACTGTACATGACCCCTAAACCTACTGATTGCAATTGGGGTAC ACCACCTTCCCCTCCTCTCAGGAGTCAGAGTGCACCAGGAACAAAAAACTTTGAGGACTGCCTTCTCTCC TCTTTGGGACTCAGTCACTTTGGGCTTTGGAGAACCCCTCCAGAGTCCCACCTTTTGGGATCACAGACAG AAAATCCACATTCCGTATAATCCTCTTGACTGTTTCTTAAGTGTGTGATGCCCATTTTCAACCTAACACA CATGTAATTGTGAGTGGTTCTTCAATGCACTAATTTTTCATAACTCTAATGTCTATTTATAAATCAGAAA AAGGGTGTTTTCATAGTTTTAGATGTTGTATGTGCGATAAACTCAAATATTTATGACTGTTGGAAGTATA ACTTGAATGCCGTCCCTTTCCTCTCTGGCTGTGAGAAAACCAATTCACTTTTAAGTTGTTTCCACTGTGA ATTTAACACAGGCGCTAAGAACTCTTAAAGGACCAAAGCATGGCACCAGTGCCATGTCCTCACACAGCAT CTGAATGTCACGGAGATGAGCCTCAGAGAGACAGTGATTGGACTGATGGTATGATGTCATGAAGTAAGCT TTCAGAAGTGTTTCTACCAAGGCTATACTCTCAAAGACAGTGGACTTTTCTTTCAAATGCTTATCAAAGC CATGTTTGTATTCTTAAAACCATAGCACTTTATTTTTTAAGTGAAACAAACTTAGACTTTAAAATTGATA AGACACGTGGAGATACTGACAACCCAGATCCACCAGTATCTAGAGAAAGACTTGTTACCCTGTATTAGTG GCTTTGGGAATTTGTTAATGTGATGGTTGTTTTTCTGCTCTGCCTTCTTATTTGGTAAACAGTTGCTCTC ATCTCATCTTGGCTGGTGTCTTGTTCAGAGTGCGGTCCCTAGACCATCGCGTCCTGGGATTCGCTTAAGC TCCAGCCCCAGCATATTCCTGCTGAGGGTCACTTTGAGGAGCGTGTAGGAGATTTGCTTACACAGAAAAC TTGGGCAACCACTGGTCTGATCAGGTGTTTATATCATTTTATGGGGTAAAATCTCAGGGAAATTAAATCT GTTCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4035998 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4035998 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12199 same embryo
	EMAGE:12202 same embryo
	EMAGE:12198 same embryo
	EMAGE:12200 same embryo
	EMAGE:12197 same embryo
	EurExpress:euxassay_007136 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source