EMAGE mouse gene expression spatial database

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EMAGE:12206

Prkaca protein kinase, cAMP dependent, catalytic, alpha ( MGI:97592)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008330. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008330_01	euxassay_008330_02	euxassay_008330_03	euxassay_008330_04

euxassay_008330_05	euxassay_008330_06	euxassay_008330_07	euxassay_008330_08	euxassay_008330_09

euxassay_008330_10	euxassay_008330_11	euxassay_008330_12	euxassay_008330_13	euxassay_008330_14

euxassay_008330_15	euxassay_008330_16	euxassay_008330_17	euxassay_008330_18	euxassay_008330_19

euxassay_008330_20	euxassay_008330_21	euxassay_008330_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12206_wholemount_strong.wlz

12206_wholemount_moderate.wlz

12206_wholemount_weak.wlz

12206_wholemount_possible.wlz

12206_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12206_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 19 20 21 22 weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

tongue muscle

weak

regional

weak expression: see section 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6187
Entity Detected:	Prkaca, protein kinase, cAMP dependent, catalytic, alpha ( MGI:97592)
Sequence:	sense strand is shown >T6187 CTTGGGCTGAGGCTCCCCCGCGGGCGGGCGCAGAGAGACGCGGGAAGCAGGGGCTGGGCGGGGGTCGTGG CGCCGCAGCCAGCGCAGCCAGCCCCAGGGGCCGCCGCCTCCGCTGCCCAGCGCGCTCCGGGGCCGCCGGC CACCTTAGCACCCGCCGCGTCGCAGCTCCGGGACTGGCCCCGGCCGCGACGCCGCCGCGATGGGCAACGC CGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAGTTCCTAGCCAAAGCCAAGGAAGATTTC CTGAAAAAATGGGAGACCCCTTCTCAGAATACAGCCCAGTTGGATCAGTTTGATAGAATCAAGACCCTTG GCACCGGCTCCTTTGGGCGAGTGATGCTGGTGAAGCACAAGGAGAGTGGGAACCACTACGCCATGAAGAT CTTAGACAAGCAGAAGGTGGTGAAGCTAAAGCAGATCGAGCACACTCTGAATGAGAAGCGCATCCTGCAG GCCGTCAACTTCCCGTTCCTGGTCAAACTTGAATTCTCCTTCAAGGACAACTCAAACCTGTACATGGTCA TGGAGTATGTAGCTGGTGGCGAGATGTTCTCCCACCTACGGCGGATTGGAAGGTTCAGCGAGCCCCATGC CCGTTTCTACGCGGCGCAGATCGTCCTGACCTTTGAGTATCTGCACTCCCTGGACCTCATCTACCGGGAC CTGAAGCCCGAGAATCTTCTCATCGACCAGCAGGGCTATATTCAGGTGACAGACTTCGGTTTTGCCAAGC GTGTGAAAGGCCGTACTTGGACCTTGTGTGGGACCCCTGAGTACTTGGCCCCCGAGATTATCCTGAGCAA AGGCTACAACAAGGCTGTGGACTGGTGGGCTCTCGGAGTCCTCATCTACGAGATGGCTGCTGGTTACCCA CCCTTCTTCGCTGACCAGCCTATCCAGATCTATGAGAAAATCGTCTCTGGGAAGGTGCGGTTCCCATCCC ACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTTCTGCAGGTGGATCTCACCAAGCGCTTTGGGAA CCTCAAGAACGGGGTCAATGACATCAAGAACCACAAGTGGTTTGCCACGACTGACTGGATTGCCATCTAT CAGAGAAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGACACGAGTAACTTTGACGACT ATGAGGAGGAAGAGATCCGGGTCTCCATCAATGAGAAGTGTGGCAAGGAGTTTACTGAGTTTTAGGGGTG TGCTTGTGCCCCTTGGGTTCTCTTTCATTTTTTCTTTTTCTTTCTATTTTTTTTCCGGTTGGGGGTGGGA GGGTTGGATCGAACAGCCAGAGGGCCCTAGAGTTCCATGCATCTAATTTAACATCCACTCCACACCCCCA GGGTTAAGGAGAGCAGGAAAGCGCTCCAGATACTGGGGAAGGGGCAACATCAGCTGCTCCCCCTCTCCCT TCTTCTCCACCCTTCCCTGCCTGTTTTCAATGAATTTCTTAGCTCCAGCCATACCCAATCTTGCTGGTGT ATCCAGGGGCAGGGTACGGAAAGAGGGCCCCAAATTCAGCCTCCTTCCCGACCCTAGCACTGGATACTAA GGATGAACGAACAGTAACGCCAACCTTCCCTTCCATGCAGCCCTACCTGGAAAGGGAGATTTTATGACCT GTACAGAGGGCTGCTTGCCAGTGGGGTTTTTTTTTTTTTTTCATTTAAATTAAGTTCCACCAGTGCCTCC CACCCTCCAAACTGTCCCACCCTCCCCAAACACCCTCCTCACTCCCTAAATCCATTCTGATGAGACCCGG GTAGCCAACTGACCCTGTCAAGGAAGGAACTGGGCTTGGAATCTCGCCCTGAGCTGCTAGCCTCCCGGCC CCCCTTTCCAGTGGTCTCATGCCAATTGTCCTGTGCATCAGCCCCCTTAAGAAGCCTCCCCCATCCTGGG CGCCTCGCTTCTAGCTTAGCTGTCAGCTGTCCATCACCTCTTGCCGTGCGTCCCCACTCACTGCAACCCC AAGTCTGATTGTGCTTTTTCTCTCAATAGAAAGGTGGGGAGCTGCTGGGGAAATTACCCCATTTATCCCT GTGTTTATCCCTCTCGTAACTTCTCCCAAAAAGGAGGAGCTCTCAGGCCTGGGTGGGGGCCCCGGGTGGA CGAGGGGGTCGTTCAACCTGTGTGCTTCGAAGGATGAGACTTCCTCTTGAACAGTGTGCTGTTGTAAACA TATTTGAAAACTATTACCAATAAAGTTTTGTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497908 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497908 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12208 same embryo
	EMAGE:12204 same embryo
	EMAGE:12203 same embryo
	EMAGE:12207 same embryo
	EMAGE:12205 same embryo
	EurExpress:euxassay_008330 same experiment
	MGI:4827416 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source