EMAGE mouse gene expression spatial database

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EMAGE:12209

Keap1 kelch-like ECH-associated protein 1 ( MGI:1858732)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007166. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007166_01	euxassay_007166_02	euxassay_007166_03	euxassay_007166_04

euxassay_007166_05	euxassay_007166_06	euxassay_007166_07	euxassay_007166_08	euxassay_007166_09

euxassay_007166_10	euxassay_007166_11	euxassay_007166_12	euxassay_007166_13	euxassay_007166_14

euxassay_007166_15	euxassay_007166_16	euxassay_007166_17	euxassay_007166_18	euxassay_007166_19

euxassay_007166_20	euxassay_007166_21	euxassay_007166_22	euxassay_007166_23	euxassay_007166_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12209_wholemount_strong.wlz

12209_wholemount_moderate.wlz

12209_wholemount_weak.wlz

12209_wholemount_possible.wlz

12209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8729
Entity Detected:	Keap1, kelch-like ECH-associated protein 1 ( MGI:1858732)
Sequence:	sense strand is shown >T8729 GCGGCCATGGCGGGGCCCCTAACGGCTAGCAGAGGAACTGTGTCTTGTCATCAGGAACCCCATGCAGCCC GAACCCAAGCTTAGCGGGGCTCCCCGCAGCAGCCAGTTCCTGCCCCTGTGGTCAAAGTGCCCCGAGGGGG CCGGGGACGCAGTGATGTATGCCTCCACGGAGTGCAAGGCAGAGGTGACGCCCTCGCAGGACGGTAACCG AACCTTCAGCTACACACTAGAGGATCACACCAAGCAGGCTTTTGGCGTCATGAACGAGCTTCGCCTGAGC CAGCAACTCTGTGACGTGACCCTGCAGGTCAAATATGAGGACATCCCAGCTGCCCAATTCATGGCTCACA AAGTGGTGCTGGCCTCCTCCAGCCCAGTCTTTAAAGCCATGTTCACCAACGGGCTTCGGGAGCAGGGCAT GGAGGTGGTGTCCATCGAAGGCATCCACCCTAAGGTCATGGAAAGGCTTATTGAGTTCGCCTACACGGCC TCCATCTCCGTGGGCGAGAAGTGTGTCCTGCACGTGATGAACGGGGCGGTCATGTACCAGATTGACAGCG TGGTTCGAGCCTGCAGCGACTTCCTCGTGCAGCAGCTGGACCCCAGCAACGCCATTGGCATCGCCAACTT CGCGGAGCAGATCGGCTGCACTGAACTGCACCAGCGTGCCCGGGAGTATATCTACATGCACTTCGGGGAG GTGGCCAAGCAGGAGGAGTTCTTCAACCTGTCACACTGCCAGCTGGCCACGCTCATCAGCCGGGATGATC TGAACGTACGCTGCGAGTCCGAGGTGTTCCACGCGTGCATCGACTGGGTCAAATACGACTGCCCGCAGCG GCGCTTCTACGTGCAGGCACTGCTGCGGGCCGTGCGCTGCCATGCGCTCACGCCGCGCTTCCTGCAGACA CAGCTGCAGAAGTGTGAGATCCTGCAGGCCGACGCGCGCTGCAAGGACTACCTGGTGCAGATATTCCAGG AGCTCACGCTGCACAAGCCCACGCAGGCAGTGCCCTGCCGCGCGCCCAAAGTGGGCCGCCTCATCTACAC AGCGGGCGGTTACTTCCGACAGTCGCTCAGCTACCTGGAGGCCTACAACCCGAGCAATGGCTCCTGGCTG CGCCTGGCCGATCTACAGGTGCCGCGCAGTGGGCTGGCAGGCTGCGTGGTGGGTGGGCTGCTATACGCTG TGGGCGGCCGCAACAACTCTCCGGATGGCAACACTGACTCCAGCGCCCTGGACTGCTACAACCCCATGAC CAACCAGTGGTCGCCCTGTGCCTCTATGAGCGTGCCACGCAACCGCATCGGGGTGGGGGTCATAGATGGC CACATCTACGCAGTCGGGGGTTCCCACGGCTGCATCCACCACAGCAGCGTGGAGAGATATGAGCCAGAGC GGGACGAGTGGCATCTAGTCGCGCCAATGTTGACACGGAGGATTGGCGTGGGCGTGGCAGTGCTCAACCG CTTGCTGTATGCAGTGGGGGGCTTTGACGGGACTAACCGGCTTAACTCCGCAGAATGTTACTATCCAGAG AGGAATGAGTGGCGGATGATCACACCGATGAATACCATCCGGAGCGGGGCCGGGGTCTGCGTGCTGCACA ACTGTATCTATGCAGCAGGGGGCTACGATGGGCAGGACCAGTTGAACAGTGTGGAGCGCTACGACGTGGA GACAGAGACCTGGACTTTCGTAGCCCCCATGAGGCATCACCGTAGTGCGCTGGGGATTACTGTGCACCAG GGCAAGATCTACGTCCTCGGAGGCTATGATGGCCACACTTTTCTGGACAGTGTGGAATGCTATGACCCGG ACAGTGATACCTGGAGTGAGGTGACCCGCATGACATCTGGCCGCAGCGGGGTGGGTGTGGCCGTCACCAT GGAACCCTGTCGGAAGCAAATTGATCAACAAAACTGTACCTGCTGAAGCACTTGGAATACCTGAGCACTG ACAACAGGACAGAAAAACAGTCTGTGTATCACTGCTTCTCTGTACTAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6404252 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6404252 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12213 same embryo
	EMAGE:12214 same embryo
	EMAGE:12211 same embryo
	EMAGE:12210 same embryo
	EMAGE:12212 same embryo
	EurExpress:euxassay_007166 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source