EMAGE mouse gene expression spatial database

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EMAGE:12212

Tmem47 transmembrane protein 47 ( MGI:2177570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008336_01	euxassay_008336_02	euxassay_008336_03	euxassay_008336_04

euxassay_008336_05	euxassay_008336_06	euxassay_008336_07	euxassay_008336_08	euxassay_008336_09

euxassay_008336_10	euxassay_008336_11	euxassay_008336_12	euxassay_008336_13	euxassay_008336_14

euxassay_008336_15	euxassay_008336_16	euxassay_008336_17	euxassay_008336_18	euxassay_008336_19

euxassay_008336_20	euxassay_008336_21	euxassay_008336_22	euxassay_008336_23	euxassay_008336_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12212_wholemount_strong.wlz

12212_wholemount_moderate.wlz

12212_wholemount_weak.wlz

12212_wholemount_possible.wlz

12212_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12212_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 weak expression: see section 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 02

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22

pons ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

ventral grey horn

strong

regional

strong expression: see section 14 15

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7140
Entity Detected:	Tmem47, transmembrane protein 47 ( MGI:2177570)
Sequence:	sense strand is shown >T7140 CAGCAGCGGACGACCTCCTTAGGGCCAGGCGTCCCAGCTAGGCAAGTCCAGTTGACCACTCGGTGTGCTT AACTGAGACCCAAGGTACGACTTCTTGGCGCGGTGTAGCGCCGCTTTCCTGTTCGGGAGGTCCCGCTCCA GGCTCTTCGGACCCTCCTGGCGTCCTCGTTTGCGTCGGGAAGGGCGGCAGCGGCGCCCGGGCATGGCTTC GGCTGGCAGCGGCATGGAAGAGGTGCGAGTGTCGGTGCTGACCCCGCTCAAGCTGGTCGGGCTGGTGTGC ATCTTCTTGGCGCTGTGTCTGGATCTGGGAGCTGTTCTGAGCCCGGCCTGGGTCACCGCGGACCACCAGT ACTACCTGTCCCTGTGGGAGTCCTGCCGGAAACCCGCCAACCTGGATATCTGGCATTGCGAATCCACTCT CGGCAGCGATTGGCAGATCGCTACTCTGGCTTTACTCCTGGGCGGTGCTGCCATCATTCTCATTGCATTC CTGGTGGGTTTGATTTCAATCTGTGTGGGATCACGAAGGCGCTTCTACAGACCTGTTGCTGTCATGCTTT TTGCAGCAGTTGTTTTGCAGGTCTGCAGCCTGGTTCTTTATCCAATCAAGTTCATTGAAACTGTGAGCTT GAAAATTTACCACGAGTTCAATTGGGGTTATGGCCTGGCCTGGGGTGCAACTATATTTTCATTTGGGGGT GCCATTCTTTATTGCCTGAACCCTAAGAACTATGAAGACTACTACTAAAATCAAGTCTGAAAAACAAACA CACATATGCATACACACACACAAAACATACACTACACACACCACATTACACACAAACACACACACACACA CACACACACACACACCACATGAAAAAGAGAAACTAACAATCCAACAAAAGATTTGTGTTGACATCTTTTC TAACACTATTTTCTAAAACACTTGTGGAGCCTCAAGCAGTTTGCTCAGTTGATTTAATATCCTTGCCTTT GGGTGTCAACATCATAACCAGCTTTTATATCCATTTTAGGGACCTGTACAAATTAAGAATGCTGATTAGA TATAGGCATATGCTGCAAACTTCCAATATGCTTATGCCATTTCTCTTGACATGTGAAGGATCTGTATGAC AGCAACCATTTTGAGAAGCTAGTTGGAATGAGAATTGCCAGAGTCTCTTTTTGCCTGCAGGGAAACAGCT GCCTAAGCAACATGTAAAAGTTCCATTGCTCTGACATTTCTACTGTTGGAGCCCTTATCACCTGTTTATA TTGCGCAGTGGCTATATTGGTTATTGGTTACTTTGCTTTGGAATTTGAAACTGCCATTTGGGCATCTAAT GAGAATCATAGATATAGCCATTTAATTCTACCTTTAAGGCTGTTCTTTTTAATAGCAAATTATACTTTAC CAATTATCATCTCTTGGGAAGGAAGTCAACGTTTGGAGACATGTGTTTTTCATTATGTAGTTATAAAATT GAGGGTGTCTGCTTTCCCCTGGGGCAATCAGAAAGAAATGTGCATGAGTTGCTTTTGTACCCTTAAAATC CTTTAAAAATTTTGCAGCAAACAATTGTCCATATGTAACAAGCTTATTCTTCTACACCACAAGCCATTAC CATACTGGTAATAAGTTGCTCATTAATAGTCCACATACATGTACCTATCATTCATATAATCAATACTTGC TCTAGTGAAGCAATTTGAACACTAACCTTGTATGCATCAACAGACTCAGTATTCACAGTTACTTACCATA CAAAAGTTCATATACCTTAAAGCTTTTGCTCTGTGTTCTCTACTGATTCATTGGAAAGTATTAATAGAAT TGCCTAAGAAGTAAAATTGAAATGTTGTTAATCTGAAATTTAATGATTCTTTTATAAGTATTGTAGTTCA AAAGAGAGTAGGAATTAGAATGATCACTTTGTGTAAAATTCATTAAAATACAAGGCTGCTATTTTTGCAA GTATTTTAAATGTCTTCATTTTAAAATAAAGGAATAGTGATAGATTTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4020610 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4020610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12209 same embryo
	EMAGE:12213 same embryo
	EMAGE:12214 same embryo
	EMAGE:12211 same embryo
	EMAGE:12210 same embryo
	EurExpress:euxassay_008336 same experiment
	MGI:4828806 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source