EMAGE mouse gene expression spatial database

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EMAGE:12274

Mapk8ip1 mitogen-activated protein kinase 8 interacting protein 1 ( MGI:1309464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011138. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011138_01	euxassay_011138_02	euxassay_011138_03	euxassay_011138_04

euxassay_011138_05	euxassay_011138_06	euxassay_011138_07	euxassay_011138_08	euxassay_011138_09

euxassay_011138_10	euxassay_011138_11	euxassay_011138_12	euxassay_011138_13	euxassay_011138_14

euxassay_011138_15	euxassay_011138_16	euxassay_011138_17	euxassay_011138_18	euxassay_011138_19

euxassay_011138_20	euxassay_011138_21	euxassay_011138_22	euxassay_011138_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12274_wholemount_strong.wlz

12274_wholemount_moderate.wlz

12274_wholemount_weak.wlz

12274_wholemount_possible.wlz

12274_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12274_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 10 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 10 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 11 18

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 16 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 03 04 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

vomeronasal organ

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36621
Entity Detected:	Mapk8ip1, mitogen-activated protein kinase 8 interacting protein 1 ( MGI:1309464)
Sequence:	sense strand is shown >T36621 AAGTACACACTGGTGGTGGATGAACATGCCCAGCTTGAGTTGGTGAGCCTGCGGCCGTGCTTTGGAGATT ACAGTGACGAAAGCGACTCTGCCACTGTCTATGACAACTGTGCCTCTGCCTCCTCGCCCTACGAGTCAGC CATTGGTGAGGAGTATGAGGAGGCCCCTCAGCCCCGGCCTCCCACCTGCCTCTCAGAGGACTCCACCCCG GATGAGCCTGATGTCCACTTCTCTAAGAAGTTTCTGAATGTCTTCATGAGTGGCCGCTCTCGTTCCTCCA GTGCTGAGTCCTTTGGGCTGTTCTCCTGCGTCATCAATGGGGAGGAGCATGAGCAAACCCATCGGGCTAT ATTCAGGTTTGTGCCTCGGCATGAAGATGAACTTGAGCTGGAAGTGGATGACCCCCTGCTGGTGGAGCTG CAGGCAGAAGACTATTGGTATGAGGCCTATAACATGCGCACCGGAGCCCGCGGGGTCTTCCCTGCCTACT ATGCCATTGAGGTCACCAAGGAGCCTGAGCACATGGCAGCCCTTGCCAAAAACAGCGACTGGATTGACCA GTTCCGGGTGAAGTTCCTGGGGTCTGTCCAGGTTCCTTATCACAAGGGCAATGATGTCCTCTGTGCTGCT ATGCAAAAGATCGCCACCACCCGCCGGCTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCTTGAGA TCAGTGTCAGGGGTGTCAAGATAGGCGTCAAAGCTGATGATGCTCTGGAGGCCAAGGGAAATAAATGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95899. Forward Primer - name:095899_F_cDNA_Mapk8ip, sequence:AAGTACACACTGGTGGTGGATG; Reverse Primer - name:095899_N_SP6_cDNA_Mapk8ip, sequence:ACATTTATTTCCCTTGGCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12277 same embryo
	EMAGE:12273 same embryo
	EMAGE:12275 same embryo
	EMAGE:12272 same embryo
	EMAGE:12276 same embryo
	EurExpress:euxassay_011138 same experiment
	MGI:4826094 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source