EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12276

Mapk7 mitogen-activated protein kinase 7 ( MGI:1346347)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011137. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011137_01	euxassay_011137_02	euxassay_011137_03	euxassay_011137_04

euxassay_011137_05	euxassay_011137_06	euxassay_011137_07	euxassay_011137_08	euxassay_011137_09

euxassay_011137_10	euxassay_011137_11	euxassay_011137_12	euxassay_011137_13	euxassay_011137_14

euxassay_011137_15	euxassay_011137_16	euxassay_011137_17	euxassay_011137_18	euxassay_011137_19

euxassay_011137_20	euxassay_011137_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12276_wholemount_strong.wlz

12276_wholemount_moderate.wlz

12276_wholemount_weak.wlz

12276_wholemount_possible.wlz

12276_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12276_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

weak

regional

weak expression: see section 05 06 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 09 17 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 08 17 18

trigeminal v nerve

weak

regional

weak expression: see section 10 16

spinal cord

weak

regional

weak expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 15

cervical ganglion

weak

regional

weak expression: see section 09 10 17

thoracic ganglion

weak

regional

weak expression: see section 13

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36620
Entity Detected:	Mapk7, mitogen-activated protein kinase 7 ( MGI:1346347)
Sequence:	sense strand is shown >T36620 ACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCATGTTGCGATTTGAACCCAGTG CCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTACCATGACCCTGATGATGAGCC TGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCAGGGAGCGCATTAAGGAGGCC ATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCGCCAACAAATCCGCTTCCAGCCTT CTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCCAGTCCCTGGGCTCCAAGTGG AGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATCCTGCACCTGACACCGTTGAT CTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAAAAGAGAGGGTGCCATCTCCGACA ATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGCAGGCTCAGAGATGGGCCCAGTGC ACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCCAGCGAGAACGAGAAGAGAAG CGCAGGAGGCGACAAGAGAGAGCCAAGGAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99584. Forward Primer - name:099584_F_cDNA_Mapk7, sequence:ACAGTATACCCAGGTGCTGACC; Reverse Primer - name:099584_N_SP6_cDNA_Mapk7, sequence:GCTCCTTGGCTCTCTCTTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12277 same embryo
	EMAGE:12273 same embryo
	EMAGE:12275 same embryo
	EMAGE:12272 same embryo
	EMAGE:12274 same embryo
	EurExpress:euxassay_011137 same experiment
	MGI:4826092 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source