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EMAGE:12287

Coro1b coronin, actin binding protein 1B ( MGI:1345963)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287
"Pseudo-wholemount" of euxassay_011049. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011049_01 euxassay_011049_02 euxassay_011049_03 euxassay_011049_04
EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287
euxassay_011049_05 euxassay_011049_06 euxassay_011049_07 euxassay_011049_08 euxassay_011049_09
EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287
euxassay_011049_10 euxassay_011049_11 euxassay_011049_12 euxassay_011049_13 euxassay_011049_14
EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287 EMAGE:12287
euxassay_011049_15 euxassay_011049_16 euxassay_011049_17 euxassay_011049_18 euxassay_011049_19
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euxassay_011049_20 euxassay_011049_21 euxassay_011049_22 euxassay_011049_23 euxassay_011049_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12287Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12287_wholemount_strong.wlz
12287_wholemount_moderate.wlz
12287_wholemount_weak.wlz
12287_wholemount_possible.wlz
12287_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12287_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36185
Entity Detected:Coro1b, coronin, actin binding protein 1B ( MGI:1345963)
Sequence:sense strand is shown

>T36185
CTACTGCCCTTTTATGACCCAGACACCAGTGTGGTCTATGTCTGTGGCAAGGGCGATTCCAGCATCCGGT
ACTTTGAGATCACAGATGAGCCCCCCTACATCCACTTCCTGAATACATTTACCAGCAAAGAACCCCAGAG
GGGTATGGGCAGCATGCCTAAGAGGGGCTTGGAGGTCAGCAAGTGTGAAATTGCCAGGTTCTACAAACTG
CATGAACGCAAGTGTGAGCCCATAGTCATGACTGTGCCAAGAAAGTCTGACCTCTTCCAGGATGATCTGT
ACCCTGACACAGCCGGGCCTGAGGCTGCTCTTGAAGCAGAGGATTGGGTGAGCGGTCAGGATGCTAATCC
AATCCTCATCTCACTACGGGAAGCCTATGTGCCCAGTAAACAACGGGACCTGAAGGTCAGCCGGCGCAAC
GTGCTATCAGACAGCAGGCCTGCCAGTTACAGCCGCTCCGGAGCCTCCACAGCTACTGCAGTTACTGATG
TCCCCAGTGGCAACCTTGCTGGGGCTGGTGAAGCTGGGAAGCTGGAAGAGGTGATGCAGGAGCTCCGGGC
ACTCCGGATGCTGGTCAAGGAGCAGGGAGAACGTATCAGCCGCCTGGAGGAGCAGCTGGGCCGCATGGAG
AATGGGGATACATAGGACCACCAGGGACCTGCTCCCGACACTGCCTCCTCCAACCCTTCCCACCTAGCTT
CTGTGGCCAGGTCACACCAGGCAGTTGGCTGCTCAGTGCCTCTGTGGCAGGTCACAGCCAGTTTGCACCT
CTCCACAGCCTGGGGCCCAGGTTGGAAGCACCACCTGACTTTTGGAAGATTTTTGTACCAAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99564. Forward Primer - name:099564_F_cDNA_Coro1b, sequence:CTACTGCCCTTTTATGACCCAG; Reverse Primer - name:099564_N_SP6_cDNA_Coro1b, sequence:GCCTTGGTACAAAAATCTTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12283 same embryo
 EMAGE:12285 same embryo
 EMAGE:12286 same embryo
 EMAGE:12284 same embryo
 EurExpress:euxassay_011049 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS