EMAGE mouse gene expression spatial database

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EMAGE:12288

Ddx51 DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 ( MGI:1916913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011011. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011011_01	euxassay_011011_02	euxassay_011011_03	euxassay_011011_04

euxassay_011011_05	euxassay_011011_06	euxassay_011011_07	euxassay_011011_08	euxassay_011011_09

euxassay_011011_10	euxassay_011011_11	euxassay_011011_12	euxassay_011011_13	euxassay_011011_14

euxassay_011011_15	euxassay_011011_16	euxassay_011011_17	euxassay_011011_18	euxassay_011011_19

euxassay_011011_20	euxassay_011011_21	euxassay_011011_22	euxassay_011011_23	euxassay_011011_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12288_wholemount_strong.wlz

12288_wholemount_moderate.wlz

12288_wholemount_weak.wlz

12288_wholemount_possible.wlz

12288_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12288_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9369
Entity Detected:	Ddx51, DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 ( MGI:1916913)
Sequence:	sense strand is shown >T9369 GGCGTGGGGCAGCTATGGCGCTGTTCCACATAGCGCGGTACGCGGGCCCAGAGGCCGCGGGGCAGGGGGA TACGGACGCAGAGGCGGGCAGCCGGGCTCGAGTGCTGCTCGAGCGGCTGCAGAACAGAGCCCGTGAGCGA CAGCAGCGGGAGCCGGAGCTCGAGACCACAGGAACGGCCGGGGAAGGGGAAGCGGCAGCAGCAGGCAAGC GGCGACGGCGGCCCCGACGGCGGCGTCGGGTGAGCGGCTCGGCGACTCCGAACTCGGAGGCTCCTAGGGC GAAGCGGCGGAAAGCGGACAAGGACGTGGACGCAGGGAGAGGTGAGGAGGCTCCGGAGGAGCTCAGTGCC GGCGCAGAGGACCCGGGTGCGAACCCTCAGGAGGATGTCCAGAGACCCCCGGCTCCAGGCCGGGTGCTGG GGGACTTTGCAAGAAGAAAGACGCCGAAGGTTCAGCCTTTCCTGCCAGCATGGTTGGCTAAGCCAAGCTG TGTCAAAAAGAGTGTCACCGAAGATCTCACTCCCATTGAGGACATCCCTGAGGTTCACCCTGACCTGCAG AAGCAGTTGAGAGCTAATGGCATCACTTCCTACTTTCCAGTCCAGGCAGCTGTGATTCCCGCCCTCCTCG AAAGTGCTGACCATGGGTTTCTCATAGGCAGAGGTGGCTACCAACCTAGTGACCTCTGCGTCTCTGCCCC AACGGGCAGTGGGAAAACACTGGCCTTTGTCATCCCCGTGGTACAGGCCCTGTTGCATCGAGTGGTCTGC CACATCCGTGCACTGGTTGTACTTCCCACTAAGGAGCTGGCCCAACAGGTGAGCAAAGTATTCAACATCT ACACAGACACCACGCCTCTGAGGGTTGCCTTGGTGACTGGACAGAAGTCGCTGGCTAAGGAGCAGGAGAG TCTTGTCCAGAAGACAGCAGATGGTTTCCGTTGCCTGGCCGACATTGTGGTGGCCACACCTGGCCGCTTG GTGGACCACATTGACCAGACTCCAGGATTCAGCCTCCAGCAGCTCCGCTTCCTGATCATTGATGAGGCTG ACCGGATGATAGACAGCATGCACCAGTCCTGGCTGCCACGAGTGGTGGCGGCAGCCTTCTACAGTGAGGG CCCCACGGGTTCCTGTGCCCTGCTCCAGAGGACACAGCCCCAGGCTCTGACTGCTGCCAGCACCTGTGTC CCCCAGATGCCTCTGCAGAAGCTACTGTTCTCAGCCACACTGACCCAGGACCCCGAGAAGCTGCAGCGCC TTGGCCTCTACCAGCCACGGCTTTTCTCCACAAGGCTGGGACAACAAAGTCCTAAAGACACAGCTGAGGT GGATGAAAACTCGGGGAAGTACACCTTCCCCGTGGGGCTCACGCATCACTATGTGCCGTGTAGACTCAGC TCGAAGCCACTCATTGTCTTACACCTGGTCCTCAGGATGAGCTGCTCAAGGGCTCTTTGCTTCACCAACT CCCGAGAAAACTCCCACAGGCTGTACCTATTAGCACAAGCTTTTGGGGGTGTGAGTGTGGCTGAATTCTC CTCCCGCTATGGGCCTGGCCAAAGGAAGAAAATCTTGAAGCAGTTTGAACAAGGAAAGATCCAGCTCCTT ATCAGCACAGATGCTACTGCTCGAGGCATTGATGTGCAGGGTGTGGAGCTGGTGATCAACTACGATGCCC CACAATATCTGAGGACCTATGTGCACAGGGTCGGGAGAACAGCTCGAGCCGGGAAAACTGGACAGGCATT CACACTTCTCTTGAAAGTGCAGGAAAGGAAGTTCCTTCAGATGGTGTCAGAAGCTGGGGTGCCTGAGCTG ACACACCATGAGATCCCCAGGAAGCTCCTGCAGCCGCTGGTTGCTCGTTATGAGACAGCCTTGTCTCAGT TGGAGAAAACTGTCAAGGAGGAGCAGAAACTGAAGGCAGCCTAGATGAGAACAGGGCCCTGGAGGGAAGA TCGTGCGAACAGAAGATAAGTTCCTTGGGGTTCCAGTGGAAACAACCCTTCTCCCCAGTGCCGTGGGCAT CTGGTCGTCTTGGTTGATGCAGCCTAGATGGTGGCCTCGTCCTGAAAAGTAATGGCTCTCGGGTTGGACT GCTGGTGTCCAGTTGGTCTTGGGAAATAAGCTCAGGTGAAGCTGAAGGATGCCTTCTGGAGTGTGAGCAA ACACTATGGTGACTGGACTGTGGTGATCAAAGTGCTTATACGTTAGCGTCTCCTGTCTACAGAGAAGCTA TGGCGAGCCTGCCCCAGGCAAGTCACTGGAGCCCAAGAACAGGTTGGCAGCAGCCTTGAAGATACTTCCT TGGCTACTAGTCTTCAAGATTGTACTTTATAATTAGCCTCCCACCCATTTAAGTTCTTAAATGATGTGAG AGTCCTTCCCTGAATATATATAGTCAAGGAGAATTAGCGCTTACCTGTGCTTTTAAGAAGGCCTGTTTTT AAAAAGTCAGTGTATCAAAAAAGATAAACATTTTCATTTGTATTCAGAAAAAAATAAATGGATCACTTGA ACCTATACAAATTCCCGATAGCTCCAACACAGCCACTTAACTACTCCTCTAACCCAGAACACCTAGGCAC AGGCTACGGGTACTATCTTCAGGCCCTTGGAACTTGGATAAGGGGAAGCTTCCCACAGCTTTAACCAGGC CTCCCTCCCTTTTAAATTTCACATATATGAGTGTTTACCTGCATGTATGTCTGTATAGCATATATGCCTA GTGCCCAAGGAAGCCAGAAGTGTTTGTTGGATCCCCTAGAACTGGAACTACAGGCAGTTGTAAGCTGGAT CCTCTACAAGAGCTTTAATTACTGAGCCATCTCTGTGTCCCTGTCCAAGTCCTTTTTAAATCTGAAGCTA CCACAGCCCTGGCTTCCCTTACTGGTGCCTTGTTCCAGTTGCCTTGGTTTTGCCTCTTCTGTGACCTTTC AGAACATGCATTGCCCTAGACCCAAGCTAGCATCACTTTCTTTCTCACAGGATCAGACCCTTCCCCTACA CCCAGAGATCTCCCAAACACTGTCTGCCAAGATTAGGTTAGTAAGATCATGTGCTGGCTTTCTATCTGAA TTGGTGATACTAAAAGGGATAGATGCTAGGATAGCTGACTGGGTGTTCACAGTGTTAGAACAAGCTTAAT AACTTGTAATTAGACCAAACCGATATATAAAGTGCATGAGGATTGCAGTTATGAGGTTTGGTTCATGTGT GTTGGGCCCAGGAAATGTTTTTCCAAAGCTTGTTTTAAGGAAATGCCTGTTTTCTAACAAAATCTTAGGT AGAGCCTAATAGTAGGAATAAAGTAGGCAAAGCCATTGTGGGAGGTTCAAGACTGACAACAGCATCAGGC ATTTTGCAGAAGAGGTTAAAACTGGGGGAATAGTTTCCACAGAAGCACCGAAACAAACGTGGGCTCATGT CAACATTAACCCTGCCAGGTAGAAGCCACTGAAGCTACCCCACAGGAGGACACAAGACAGTGAAACATTT GGAGTAACTCACTCATCACACTATAGGGCCTGTTTGTTTCCCAGGCCTGTAGTGGTTACCTTACAGCTTT CTCTGCCCTGGACCATAGCTCTGTGGAATTTGCATCTGACCCTAGCCTCTTGCTGTGTGGGAGGTTGCTC ACTGGGGAGTAGTACCCAAAACTGAGAGAGTCAAAGTAGCCGGTGTCATTCTGATTCTTTTTTTTTTTTA ATGTTTATTTTGTTTGCATGTTTATTTGTGTACCATGTGTTTGCAGTCCATTATGGAAACCAGAAGAGGG GGACTGAGTTACAGTCAATTATGATCTACCATTTAGGTGCTGGGAATTGAACCTGGGTCCTTTGGAAAAG CAGCCAGTGTTCTTAACTGTTGAGCCATCTCTCCAGCCCATCATTGTGTCCCTTAAATCACAGCCTTTAT GGGTAAATACTGATCTCTCACAGGGTAGTCACAGTTGATTTTTGAAAACAGCTGGTTTTTTTAAAAAAAT GTTTGTTTACTGAGCAGGGTTCTCTGCATAGCCTGGCTGTTCTGGAACTTGCTCTGTAGACCAGCAGCCT TGAACTCTCCTGCCTCTGCCTCTTGAATGCTGAGATTAAAGGCCTGTGCCACCTTCACTCCTAGCCAGAT ACAGATGGTTTTAATGTGAGTTATTAGAGCTAGGATTTTCACAGACCAAAACTGAAAGATCTTGCTGATG AGAAGTCTGGTCTGAAGGTCAGACTTCCTTTTGAGTAATCAACATTGTGTTACTAATGCTTTCAGCATTT CATACAGTGAAATTTGATCATATTCACACACACACCCTTTTCTCCCATATCCATCCTTACTCTCACCTCC CCACCCATCTAGCCCCAAGGTTTTCTGTTGTTGTTTAAACCCATTGAATTGATTTGTATTATGGAGTGTG GTTCCTGCCCTGCAATGTGGTCAGCCTACTAGGGGTCACACTACCAAAGAAAACTGAGTCCCTCTTTCAG TAGCAGTAATAACAGTAGTTCCTCAGCTTATACAGGTCTTGTGCATGCCATCACAGTCTCTGTGAGTTCA TATGCCTTACTGCTCTATTGTGTCTTGAAAGCATTTTCCCTTGAAGTTATGTACTGCCTCTGGCTCTTAA ATTTTTTTTGTACCCTCTTCCTTAAAGATCCCTGAGCTTGGACAGAGAGGTGTGAAATGTTCCACTTAGA GGTCAACACTCCACACACACACTCTCACTCTGGACTTGTTGAGCAGCTGTCATCTGAAAGCTTTTCTGAT GAAAGTTGGCAGTACACCTACCTATGGGGATAGCAGTGTCATTAAAAGTCATTTTTTACCCTGTAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853826 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853826 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12289 same embryo
	EMAGE:12292 same embryo
	EMAGE:12290 same embryo
	EMAGE:12291 same embryo
	EurExpress:euxassay_011011 same experiment
	MGI:4824273 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source