EMAGE mouse gene expression spatial database

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EMAGE:12289

4931406P16Rik RIKEN cDNA 4931406P16 gene ( MGI:1924311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011009. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011009_01	euxassay_011009_02	euxassay_011009_03	euxassay_011009_04

euxassay_011009_05	euxassay_011009_06	euxassay_011009_07	euxassay_011009_08	euxassay_011009_09

euxassay_011009_10	euxassay_011009_11	euxassay_011009_12	euxassay_011009_13	euxassay_011009_14

euxassay_011009_15	euxassay_011009_16	euxassay_011009_17	euxassay_011009_18	euxassay_011009_19

euxassay_011009_20	euxassay_011009_21	euxassay_011009_22	euxassay_011009_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12289_wholemount_strong.wlz

12289_wholemount_moderate.wlz

12289_wholemount_weak.wlz

12289_wholemount_possible.wlz

12289_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12289_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ovary

weak

regional

weak expression: see section 04 05 21 22

lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9363
Entity Detected:	4931406P16Rik, RIKEN cDNA 4931406P16 gene ( MGI:1924311)
Sequence:	sense strand is shown >T9363 GTTCCTGCCTACACTGAAAATATTAATTATATAAATCTGTTATTTTTCTCACCTTTATGTTGGATCACAT GGTCACCTGCCTCATGGAAATGCCTTTTTTAAAACTTAGATTTGCAGAACTCCACTATTTTTATACCTAG CTACAGTTTTGGAAAAAAAAAAAGGACTCAGAACCCTGACCTGCCAGTGGTCCCCGGGACAGTTCCCCAC CCGTATGTATTGCTGCAGTGCCCAGGACAATAAGATGGACTACAAGCGGCGCTTCCTGCTTGGCGGGTCC AAGCAGAAGGTGCAGCAGCACCAGCAGTACCCGATGCCTGAGCTGGGCCGAGCACTGAGCGCTCCCCTGG CATCCACCGCCCCCTCTGCCCCGCTGGGCAGTCTGACCGCTGCAGGCAGCTGCCACCATGCCATGCCCCA CTCTACTCCCATTGCTGACATCCAGCAGGGCATCTCTAAGTACCTGGATGCCCTCAATGTCTTTTGCCGT GCTAGTACGTTCCTCACAGATCTCTTCAGCACCGTGTTCAGGAACTCACACTACTCAAAGGCCGCCATGC AGCTCAAAGATGTGCAGGAACATGTAATGGAAGCAGCCAGCAGATTGACCTCAGCCATAAAGCCCGAGAT TGCCAAGATGCTCATGGAACTCAGTGCTGGGGCTGCAAACTTCACAGATCAGAAGGAATTCAGTCTCCAG GACATTGAGGTGTTGGGGCGATGTTTCTTGACAGTGGTGCAAGTCCATTTCCAGTTTTTGACCCACGCCT TGCAGAAAGTCCAGCCGGTGGCTCACTCTTGCTTTGCTGAGGTAGTTGTGCCAGAAAAAAAGAACAGCAG TCTGTCTGGCATGGGCCACACACCTGAACTGGAGGAAGCTGTGCGATCCTGGCGAGGGGCAGCTGAGGCG ACATCTAGATTGAGAGAGAGAGGCTGTGATGGATGCCTGGCAGGAATCGAAGTCCAACAACTCTTTTGTT CTCAAAGTGCAGCAATTCCTGAGCACCAGCTAAAAGAGCTAAACATAAAAATCGACAGTGCTTTGCAGGC TTATAAAATAGCTTTGGAAAGCTTAGGCCACTGTGAATATGCAATGAAAGCTGGCTTCCATCTAAACCCA AAGGCCATTGAAGCAAGTTTGCAGGGCTGCTGCAGTGAGGCGGAAGCTCAGCAGACAGGGCGAAGGCAGA CACCCCCACAGCCCATGCAGTGTGAGCTCCCCACGGTTCCTGTGCAGATAGGATCGCACTTCCTGAAGGG GGTCTCCTTTAATGAGTCGGCTGCCGACAATCTGAAGCTTAAGACGCATACCATGTTACAGCTCATCAAG GAAGCAGGCTGCTACAATGGAATCACACCTAGGGATGATCTTCCTGTGACTGAAGTCCTGAATCAGGTGT GCCCGTCCATGTGGCGGGGTGCCTGTAAGACTGCTGTGCAGCTGCTGTTCGGCCAGGCAGGGCTGGTGGT TGTTGACACAGCACAGATTGAAAATAAAGAAGCCTATGCACCCCAGATCAGTCTTGAAGGTTCCAGAATT GTGGTTCAAGTCCCATCCACATGGTGCTTAAAGGAAGATCCTGCTACCATGTCCCTGCTGCAGAGGAGCC TGGATCCCGAGAAGACCTTAGGTCTGGTGGATGTGCTTTACACAGCCGTGCTAGACCTAAACCGCTGGAG AACAGGGAGGGAACAAGCTTTACCCTGTATACAGATCCAGCTGCAAAGGGATATCTGCGATTTTGGGAAT CAGGCTGACCTGCCTTCTGGAAATGGAAGCAAATCCTCGGGACTGCAGAAGACTTTCTCCAAACTGACAT CCCGGTTCACAAAGAAAGTGTCATGCACCAACTCTGGCAGCACACACTATTCCAAAAACATCTTCACAGC TGGGTGTTCAGAGGAAAGGGCCAAAATGATGGGCAATAATATTGATACACGGTTACAGAGCATTTTGAAC ATTGGTAACTTCCCTAGGACTACAGACCCTTCACAGTCGTCTCAGAATTCCAGTAATCCAATGGTCAATG GCTTTCTCCTGGAGAGACGTGAGAACTTCCTGCATGGGGATGATGGCAAGGATGAGAAGGGCATGAACTT ACCCACTGATCAGGAAATGCAGGAAGTGATAGATTTTCTCTCAGGCTTTAACATGGGCCAGTCCCATCAG GGGTCCCCACTGGTGACAAGGCGTAACTCTACTGCCACTGCCATGGTGACTGAACAGAAGACAGGAACCA TGCAACCACAGCAGCCATCACTGCCATTGGCTCCTCCACTACGGCCACCCCAGGCTGGAGCACACACGCC TCTGGCAGCCCAGCAAGGTCTGGCTCCTCAGCAGCAGTCTCCAAAGCAGCAGCAGCCCCAAGTCCAGTAC TACCAGCACCTGCTGCAACCCATTGGATCACAGCAGACTCCACCTCAGCCTCGGGCGCCTGCGAAATGGG TACATGGCTCATCTCAGCACCCATCACAGCCCATGGCAGCGGGTCTGTCTCCTCTTGGTCAGTGGCCTGG CATATCTGATCTCAGCTCTGACTTGTACAGCTTGGGTCTAGTGAGCAGCTATATGGACAATATGATGTCA GAAGTTTTGGGACAGAAGCCCCAGGGACCTAGAAATAACACCTGGCCAAACCGTGACCAAAGTGATGGAG TCTTTGGAATGCTGGGAGAGATTTTGCCTTTTGATCCTGCAGTGGGCTCAGACCCAGAATTCGCACGCTA TGTGGCAGGAGTGAGCCAGGCAATGCAGCAGAAGCGGCAAGCTCAACATGGCCGCCGGCCAGGCAACCCC CGTGGCAACTGGCCGCCCATGGAAGACGCTCATCGGACCTGGCCTTTGCCAGAGTTCTTCACAGAAGGGG ACAGCTTGCACAGCGGCTGGTCAGGAGCTCAGGGAGACTCGGCCAGTTCCAGTGATGAGACCTCTTCAGC CAATGGGGACAGTCTGTTCTCCATGTTTTCAGGGCCTGACCTTGTTGCTGCTGTTAAACAGAGAAGGAAA CACAGCAGTGGAGAGCAGGAGACCAGCACACTGCCCTCACCACCTCTCCTGACCACAGTGGAGGACGTGA ACCAGGATAACAAAACCAAAACGTGGCCACCCAAAGCACCATGGCAGCACCCTTCCCCGATGCCCAGCAC GCTGCCCAGCCCAGCTGCACCACTCTATGCAGTCGCCAGTCCTGGCAGCCAGTGGAACAACACCGTGCAG ATGCTGCAGTCCCCTGTGTGGGCTGCAGCCAATGACTGCAATACCACCTCCTTCACCTATGTTCAGACAC CACCACAGCCCCCACCTCCACCAGCACATAAGGCAGCACCCAAGGGCTTCAAGGCCTTCCCTGGGAAGGC TGAACGTAGGCCAGCCTATTTGCCCCAGTACTGACTACAGCTAGCCTGCTGCCCATCAAAGCTGTTGGGG CAACTGTCCCCAAGGCTAACTAGCTGATACTAGCCCCCAGAAGGACCAGTACACCCTGTCCCAGGGACAG AAATATTCCTTGGGTCTGAGGTGATTGGCAGCTCCAAGTCTTTAAAGCCTGGTTTCTCAAACTTTGAAGG TAGCAGACCCCTGAGAGCCAGCTGAAGATGGAGATTTCTGACACTCAGGGGATGCTGGCAATCTGCATGC TTAATAAGCACCTCAGGTAATCCTGGTGCAGGTGGCCAGAGGCCACACTTGGGAAAACACAGTCTGAAGG TAGTCCTGCATGTGGGAAAGGCATGAGGCCTGCAGGCTGAGAACAAAACTGACCATTCTTTGAAACTCTT CTCTTTAACACACTGAGTGGGACCCACACCCATCACTAATTTTATAGATATTTTTCATGTAGGCTTTCGG TTAAAACGTCTCCTGCCTAAAATGCAGTGGCTATCTTAAGATCCAGGTACACTGGCTGAAGGTTAGTTAG CTCAGCACTGTCTTCATTTAAGCTTATATAAGATGCTAGTGATCAGAAGCAACCACCTGGCTTCCCATGG GTCAGTAGCTCCTCTGAAGCACCCAGGGGTGCAGGCTCATGTAATGTACAAGAGCGGGATTGGGCAACTG CTGTCAGGCATATTCTGGCCTCCCAGGGGGTGCAGCTGGTGCATGCTGAGTGCACCTGCTGCCCTCACTT CCTTCCACCATGACCAGGGCAGCACCAGCACCACGCTCCTCTACGGAAGGAACTGTGGAGGGGAGGGGTT GCACCAAGACCCCCACACTCAGAACCACAGTGACATTGTGAGCTGACTCAGAGACAAAGGAATTATGATG TAGACAGGAATTCAAAATCTTTGTTTAGTCACCAACAACACCTCACTTTTAGTTAATGTGTGCCATTAAA CAGATGACACTCCATGGATTTGGGGATATTTTCCAGCTAGAATGGATCCAGAATTGAATGGTGCTTAAAT TGAGAAATAATAAATATATCTATATAGAGTAGACATTTCCCACTATATATTAATTGAGGTTGCAGAAGGT TCTTTATATAAAACTTATTTAAAATTTTCATATTTCATCTTTGAAAATGTCTAATTGAGAAAAATCCATG ATATTTTCTGGTATGAAAGTTTGACAGTATTAATTTTTTTATATTTTCTTAAATCATTAAACCATTTTAA TATATGTTAACTACTAATAAATGGTTTATTCTTTCTAACTCCATGTAAGCTTTTCCAATGAAGATTGTAA TAACACATTTTTGTTCTCATTTTTCTACAGATAAATTTATATTTAAAAATACCAAAAAAAAAAATCCCTA TGTATACACACCACACACATACACACACACAGACACACATTCACTTATAAGAAAATTATAGAGGACCTGT AGGGTGAGCCCAGCCACCTGCGTTTTTAGTACTGTGTCTCCAGGCTGCCTGAGGCCTCAGCTGTTGTCAT TCGTGCTGTGTGGGGGTGACATTTCTGCCTGTAGTGAAGATCCTTCTCAGTGGGTTCGGAATATTCACCT CACCATGTTTACAGAGAACTATTTTGTACATAGAGTTTTCCAGGCACGTACTTTGCACCAGCCCTGCGTG GCTGTTGTCTGTGTTAGCTGTCACAGTGTGCACACTAATCTGTTCAGAGTTCCTTGTGGCTGTTTGACTT GTAAAAACTGTGTATTTCCTTCAGTAATGTGTCCACAGTGCTGTGTTTCCAGTTCTAGTCTTCTGCAGTA CTCTCATTTTCATAGTGCCTCCCTGAAGACCTCACCTTGGGCCAAGGTCACTCCTCGGTGCCACAGCACA GGTGACACTGCTAATTGTCATCTCTGGTCTGTCTTTCTCTATTAAGGAAGATATTTGTCTCCACGGCTGT GTGACCGTGTGAATCAGCTTGTGTAGTCTCCACATGGGTGCTGTTCGAAGCACCACCTTGCTCTAAACAC TGGTGATTCCAGGTGCTGCGCTTGGCAGAGGTGTCTTGCCAAAGCGCATGTATGCGTGTGAGTGTGTCCT CAGCACGGCCAAGCATGGCTGCCTTGCTCGGCATTAGCAGGACATCATGTGAATAGTTGTGACCTTCCAA AACTGGAACTCTACATTTTGTATCTTACTTTTAAAGCTCCTATAAGTAAAATAACTTGGCTTTATTAAAA TATACATTTAATAATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6852905 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6852905 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12292 same embryo
	EMAGE:12288 same embryo
	EMAGE:12290 same embryo
	EMAGE:12291 same embryo
	EurExpress:euxassay_011009 same experiment
	MGI:4822746 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source