EMAGE mouse gene expression spatial database

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EMAGE:12293

Zfp12 zinc finger protein 12 ( MGI:99157)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010948. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010948_01	euxassay_010948_02	euxassay_010948_03	euxassay_010948_04

euxassay_010948_05	euxassay_010948_06	euxassay_010948_07	euxassay_010948_08	euxassay_010948_09

euxassay_010948_10	euxassay_010948_11	euxassay_010948_12	euxassay_010948_13	euxassay_010948_14

euxassay_010948_15	euxassay_010948_16	euxassay_010948_17	euxassay_010948_18	euxassay_010948_19

euxassay_010948_20	euxassay_010948_21	euxassay_010948_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12293_wholemount_strong.wlz

12293_wholemount_moderate.wlz

12293_wholemount_weak.wlz

12293_wholemount_possible.wlz

12293_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12293_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8427
Entity Detected:	Zfp12, zinc finger protein 12 ( MGI:99157)
Sequence:	sense strand is shown >T8427 GTCGCGAGCCTGCGGGCCGAGGCCGGTGCGTCCGGGTCGGCAGCAGGCGAACGCAGCTTTGCAGGCCGGG GAGGCCGAGGGCCGGGCTGACGGGGGCAGCGAGACCCGGGCCCGGCGCGGCCTCCTAGACCCGGCTGCAT TAGTCCTTCTACAGACTTTTCCTACTGCAGCGGAAGATGAATACATCCTTAGGCCCACTGTCATTCAAGG ATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGCAGCTGGACCCTGAGGAAAGGACGACATACAGGGA TGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGTGGGTGAGGACCACTTGCTTCCCAGGTGCTCGGAT GTGGGACGCCCATCAGAGCTGATGCCTTTAGCTTTGAGGGTATGACATTATCAAACCGGATGTTATCATC AAGTTGGAACAAGGAGAAGAGCCATGGATAGTAGAAGGAGCGTTCTCGCCTCAGAGCTATCCAGATGAGA TCCGGCACATGAGCAGGCTGATGGAGGAGGACCAGGGAGGTGAAGAGAACCAGTCAAGTTCAGCTGTGTT CAGCTACAGAAGCCGGGCTGATGCCTCTAGTAAGGCCACTGATGGAGAAACAAAGCCCTTTCCCTCACAG AAGGCCCTCCCTCAGTGCAACTCCTGTGAGAAGAGCCTGATGTGTGTCTCGGCGTTCATCCGAAGCGACG GAAGCTACGCAAAACTGAGGCCCAATGTGTGTGCCGGGTGTGGGAAGCCCCTCCCCTGCAGCAAGCCCGA GGAAACACACCCTGGAGGTGAGTCTTACGAATTTAGTGGGGATGGAGATGAGGACCCTCTCGGTGAAGAA GGCGTGTATCAGAAGGGTCACTTTTTGGAGGAGCCCTTTGAATACGTTGAGTGCCAGAAATCCTTCCCCA AGGGCACTGTGTTTCTTAATCACCTGGAAGAGGAGCCCTGCGACTGGAATGACGCTGAGGTCGCTTTCCT GCAGACGTCAGACCTCAGTGCCCACCAGGATTCTCTCATGGAAATGAAGCCCTACGAGTGCCAGCAGTGT GGGAAGTCCTTCTGCAAAAAGTCCAAGTTCGTCATCCATCAGAGGACTCACACAGGAGAGAAGCCCTTCA AGTGCAGTCAGTGTGGGAAGTCCTTCTGCCAGAAGGGCACCCTCACCGTCCACCAGAGGACGCACACCGG GGAGAAGCCCTACGAGTGTACCGAGTGCGGGAAGACCTTCTACCAGAAGCTGCACCTCATTCAGCACCAG AGGACCCACTCGGGCGAGAAGCCCTACAAGTGTGGCTACTGCGGGAAGTCCTTCTGCCAGAAGACGCATC TCACTCAGCACCAGAGGACGCACTCGGGAGAGAGGCCCTATGTTTGTCACGACTGTGGCAAGACGTTCTC TCAGAAGTCAGCACTCAATGACCACCAGAAGATCCACACGGGCGTCAAACTCTACAAATGCAGCGAGTGT GGCAAGTGCTTCTGCCGCAAGTCCACGCTCACAACCCACATGAGGACGCACACGGGCGAGAAGCCGTACG AATGCAACGAGTGCGGCAAGTTCTTCTCCAGGCTGTCGTACCTCACCGTGCATTACAGAACCCACTCGGG GGAGAAGCCCTACGAGTGTGCGGAGTGCGGGAAGTCCTTCTACCTGAACTCAGCCCTTATGAGACATCAG AGGGTGCACACGGGGGAGAAGCCTTACGAGTGTAACGAGTGTGGGAAATTATTCTCGCAGCTGTCCTACC TCACCGTGCATCACAGAACTCACTCAGGAGTGAAGCCCTATGAGTGTAGTGAGTGTGGAAAGACCTTCTA CCAGAATTCAGCCCTCTGTAGACACCGGAGAATTCACAGAGGGGAGAAGCCCTATGAATGCTACATCTGT GGCAAGTTCTTTTCTCAGATGTCTTACCTCACGATCCATCACAGAATTCACTCAGGGGAGAAGCCCTATG AGTGCCGCGAGTGTGGGAAGTCCTTCTGCCAGAACTCAGCCCTCAACAGACACCAGCGCACGCACACGGG AGAAAAAGCCTACGAGTGTTACGAGTGTGGGAAATGCTTCTCCCAGATGTCTTATCTCACTATCCATCAC AGGATCCACTCGGGAGAGAAGCCCTTCGAGTGTAACGAGTGTGGGAAAGCCTTCTCTCGGATGTCGTACC TCACTGTGCACCACAGGACCCACTCAGGAGAGAAGCCCTATGAATGTACGGAGTGCGGGAAGAAATTCTA CCACAAGTCCGCGTTCAATAGCCATCAGAGAACTCACAGGAGAGGGAGCGGGAATGGGGTTGATTGATGT GGGACGGCTCCTTTACAGCCAGACTCAACCGTCTCAGTAAGCTAGAAACCCCTGTCTGCCGCTGAGCCCT GCTGTACGTGGCCCCAGGTACCCATAGGCTCCTTCTCTGAGGAGTCAATCCTCCGAAACTACATAGGGAA AAGGGTTTCTCTGTACTGAATGTGTACAGAATATTTTTTGTCTTTCTTCCCTAAGTAGTACAATATAACA GCTCTTTATACTGCGTTTATATGGTACTAAGCGTCATAGATATTCTAGAGGCGAGTTAAGTACAGAGGGT GTGCACAGGCTGTCACGATGTCATCTTACACAAGGGCTTACTCTGCAGACGCTGGTGTCCCGCTGGTCTC AGAACCACACTCGGGCACACCGAAGGATACCTCTGTCAGAGAGCTCACGCAAATAAGCATGAGCAAGTCT GTGAGAGATAGATTCATCAGAAACCCACAGGGCAGGAAGCACAGTTGTGAGAAGAGCACACAGCAGGCTA CACAGGAGTCAGAATGTATAAATCCGTCTCAGCATAACTTAAACTGCTCATCAGGGAACTGGCTCGACTG AAGGAGCGGCGGAAGCACACGCCTTTGGTAACTGCTAATACTAAAGGACATTTTTGTAAAAATTAAAAGA AGAGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTTTTCCAGAGGTCCTGAGTTCAAATC CCAGCACACACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGGTGTGTCTGAAGA CAGCTACAGTGTACTCATATACATAACATAAATACATTTTTTAAAAAATTAAAAGTAATACTCTGCTAGA AATAAGTACACTCTATAACTGCAGGATGTAAAGTTGCCAGTCTGGTTAGCTGAGTTTGCCACACTAAACT GGTAAGTGATTCCCTAGGGGCAAAGGGCTTGGGGGTCTCTTGTTTTTGTTTCAGTAGCCATCACAGAATT CTCATGGTCAGTTTTAATCCTGTTTGGAAAAGTGTTGTGTTGTTACTTTGTAACATATGGAGAGCTTACC CGTTCTAAGGAAGGTCCTCATCCTGTCATTATGTTCATATGCACTGTCACTGAAGTTATGAACTATGCTT TTTCTCATATTCTTCCTTGGGTTTTCAAATGCATTGGGTCATTGTCACAGGATTCTATCCTCTGTAAAGA AGCCAGGTTATCCCCCGCAACTATAGCAAAGATGGAATGTATCTAGAAAATAGAAAACTCTGAATAGATC ATTTTAAACTAAGCCAGAAGTCTGTAAAGGTGAGGAATAGCCTTAGCACTCCTGTACTCTGTACAACAAA GAACTAGACTTAGGGTGTTGTCATTTCTACACGCCATTGGCTCGCTTGTCTCACGTGGATGCTTACGCGT GTGTAGAACTCAATGGCTGGGTAGCACGGTACGTGCTGTGGTTACCGCCGTGCTCTGTTCTCCTGTCCCT CGGAAGTGAGCGCACACACAGCGGACTGTGGCAGACTTCACCCAGTGCCTTCTGCCACTCCCTCCTTGGC TTTCGTATGTCGTCAGAGTGCATTCCTCCTTAGATGATGGGACGCAGCTCCCGGGAAGTTTTCATTGTGG GGGTGAGCCTTTCTCAGAACCTTGGACCCAAGTCCAGAGAGTTCGAAAAGGTCATTTCTTGAAAGGGTCT CCTCAAAGCAGCAGAGCCTTAGTGAACATAACCTTGTCTGGATGCGGTGCCTGATGATGACTGCCAACCA TGTATACCCTGTAGTCATGACGACAGAAGGTCGGAATGACGTCACAGAGCCACTAAAGCAACGTGACCTG TAGCCACTCCGTGTCTGGACTGTGGGTTTTGTTTTTTGTTTTTCTGAGAGACTGTTTCTAAACCAGGTGA CTTAGGGACATTCTGTTAATAACAACAGGACAGCCTGGTGCCCCATTACACTCTGTAGGAGTTATGCAAT TCAGAAGTGGAATTTAGGAAATCATTTATTTTGATGTGTCAATAGAGAGATTTATTGTCTGTAAATTTGG ATGTAAATAATCTGATCATTGAAAATGAACTTGAAACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5697949 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5697949 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12298 same embryo
	EMAGE:12294 same embryo
	EMAGE:12297 same embryo
	EMAGE:12295 same embryo
	EMAGE:12296 same embryo
	EurExpress:euxassay_010948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source