EMAGE mouse gene expression spatial database

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EMAGE:12307

Ifit3 interferon-induced protein with tetratricopeptide repeats 3 ( MGI:1101055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006975. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006975_01	euxassay_006975_02	euxassay_006975_03	euxassay_006975_04

euxassay_006975_05	euxassay_006975_06	euxassay_006975_07	euxassay_006975_08	euxassay_006975_09

euxassay_006975_10	euxassay_006975_11	euxassay_006975_12	euxassay_006975_13	euxassay_006975_14

euxassay_006975_15	euxassay_006975_16	euxassay_006975_17	euxassay_006975_18	euxassay_006975_19

euxassay_006975_20	euxassay_006975_21	euxassay_006975_22	euxassay_006975_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12307_wholemount_strong.wlz

12307_wholemount_moderate.wlz

12307_wholemount_weak.wlz

12307_wholemount_possible.wlz

12307_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12307_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9994
Entity Detected:	Ifit3, interferon-induced protein with tetratricopeptide repeats 3 ( MGI:1101055)
Sequence:	sense strand is shown >T9994 GGACTGAGATTTCTGAACTGCTCAGCCCACACCCAGCTTTTCCCAGCAGCACAGAAACAGGTCACCATCA TGAGTGAGGTCAACCGGGAATCTCTGGAAGCGATCCTTCCACAGCTGAAGTGCCATTTCACCTGGAATTT ATTCAGGGAAGGAAGTATGTCCAGTCATATGGAAGACAGGGTGTGCAACCAGGTCGAACATTTAAACTCT GAGGAGAAGGCAACAATGTATGACTTATTGGCCTACATAAAGCACCTAGATGGCGAAAGCAAGGCCGCCC TGGAGTGCTTAGGGCAAGCTGAAGATTTAAGGAAGTCAGAGCACAATGATCAATCAGAAATTCGTCGACT GGTCACCTGGGGAAACTACGCCTGGATCTACTATCACATGGGCCGTCTCTCAGAAGCTCAGGCTTACGTT GACAAGGTGAGACAAGTTTGCCAAAAGTTTGCGAATCCTTACAGCATGGAATGCCCAGAACTTGAATGTG AGGAAGGATGGACACGCCTAAAGTGTGGAAGAAATGAACGAGCAAAAATGTGCTTTGAAAAGGCTCTAGA AGAGAAGCCAAAGGACCCAGAGTGCTCCTCTGGGATGGCCATCGCCATGTTCCGCCTAGAAGAAAAACCT GAGAAGCAGTTCTCCGTGGATGCTCTGAAGCAGGCCATGGAGTTGAATCCTCAGAACCAGTACCTGAAAG TTCTCCTGGCCCTGAAACTGCTGAGGATGGGAGAAGAAGCTGAAGGGGAGCGATTGATTAAAGATGCTTT GGGGAAAGCTCCTAATCAAACGGATGTCCTCCAAAAGGCAGCTCAGTTTTACAAGAAAAAGGGTAACCTA GACAGAGCTATTGAGTTACTTGGAAAAGCACTGCGATCCACAGTGAACAACAGTCCTCTCTACTCTTTGG TCATGTGCCGTTACAGGGAAATACTGGAGCAGCTACAGAATAAAGGAGATGCTGACAGCAGTGAGAGAAG ACAGAGGATGGCAGAACTGAGACGATTAACGATGGAGTTCATGCAGAAGACTCTTCAGAGGAGGCGAAGT CCTTTGAACTCCTACTCAGATCTCATCGATTTCCCGGAAGTAGAGAGATGCTATCAGATGGTCATCAGTA AGGAGAGCCCCGATGTTGAGGAAGAAGACCTCTATGAGCGCTATTGCAACCTCCAGGAGTACCACAGGAA GTCTGAAGACCTCGCAGCCCTGGAGTGTTTGTTGCAATTTCCCAGAAATGAAAGGTCAATCGAGAAGGAA GAGGTTAAAGAGCAAACATAGCAAGCAGATCTTAACCTCCAGTAGCAAATTGTGGTGGATTCTTGGCAGT TGCAGGGATAAAGGAGTGGCTGAATGGTTTTGGGGTTTGGGAGGCAACGCACTTTGGGGCACAGGCAGGC TTTTCCTGGCACCATGAACCTGAGGACAACCGGAAGTGTGTCAGAGTGCAGACAGACAGTCTCTCAGCTC TGTACTGTGAGACAGATGTGCTGTGGAGTGCTGCTTATGGGGAGAATGTGCTGAAAAAAGCATGAGCCTT CCTGCCAAGGATTGCTGACAAACTGCTCTTGATTGTTTCTTTAAGGAACTGCTTTCTCTCCCTGACTCCT CTGCTCATCCTAGCCATACAATTTTCCAGTCAGCAAACCTCATTACTAATCATGTAGGGAATGGAGCTTA AAGCAGACAGAGCACCTTTTGATCACATTTTTTTTCTCTGCAATAAATGACAACTCCCAACTAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30317428 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30317428 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12308 same embryo
	EMAGE:12305 same embryo
	EMAGE:12306 same embryo
	EMAGE:12309 same embryo
	EMAGE:12310 same embryo
	EurExpress:euxassay_006975 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source