EMAGE mouse gene expression spatial database

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EMAGE:12309

Nr1i3 nuclear receptor subfamily 1, group I, member 3 ( MGI:1346307)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006978. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006978_01	euxassay_006978_02	euxassay_006978_03	euxassay_006978_04

euxassay_006978_05	euxassay_006978_06	euxassay_006978_07	euxassay_006978_08	euxassay_006978_09

euxassay_006978_10	euxassay_006978_11	euxassay_006978_12	euxassay_006978_13	euxassay_006978_14

euxassay_006978_15	euxassay_006978_16	euxassay_006978_17	euxassay_006978_18	euxassay_006978_19

euxassay_006978_20	euxassay_006978_21	euxassay_006978_22	euxassay_006978_23	euxassay_006978_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12309_wholemount_strong.wlz

12309_wholemount_moderate.wlz

12309_wholemount_weak.wlz

12309_wholemount_possible.wlz

12309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10001
Entity Detected:	Nr1i3, nuclear receptor subfamily 1, group I, member 3 ( MGI:1346307)
Sequence:	sense strand is shown >T10001 ATGCAAGAGAAGGCCCTTGTTTTCCAGGCACTGAGGACCGCAGTCCCTAATTCCTGGCAGTTCCTGAGAT CTCAAGGAAAGCAGGGTCAGCGAGGAGGCCTGGGGAGAGGCATCCTACACCCGATCTTGTGGCCTGCTGC CTAAGGGAAACAGGAGACCATGACAGCTATGCTAACACTAGAAACCATGGCCAGTGAAGAAGAATATGGG CCGAGGAACTGTGTGGTGTGTGGAGACCGGGCCACAGGCTATCATTTCCACGCCCTGACTTGTGAGGGCT GCAAGGGCTTCTTCAGACGAACAGTCAGCAAAACCATTGGTCCCATCTGTCCGTTTGCTGGAAGGTGTGA GGTCAGCAAGGCCCAGAGACGCCACTGTCCAGCCTGCAGGTTGCAGAAGTGTCTAAATGTTGGCATGAGG AAAGACATGATACTGTCAGCAGAAGCCCTGGCATTGCGGCGAGCCAGACAGGCACAGCGGCGGGCAGAGA AAGCATCTTTGCAACTGAATCAGCAGCAGAAAGAACTGGTCCAGATCCTCCTGGGGGCCCACACTCGCCA TGTGGGCCCCATGTTTGACCAGTTTGTGCAGTTCAAGCCTCCGGCCTATCTGTTCATGCATCACCGGCCT TTCCAGCCTCGGGGCCCCGTGTTGCCTCTGCTCACACACTTTGCAGATATCAACACGTTTATGGTGCAAC AGATCATCAAGTTCACCAAGGATCTGCCGCTCTTCCGGTCCCTAACCATGGAGGACCAGATCTCCCTTCT CAAGGGAGCGGCTGTGGAAATATTGCATATCTCACTCAACACTACGTTCTGTCTTCAAACAGAGAATTTC TTCTGTGGGCCTCTTTGCTACAAGATGGAGGACGCAGTCCATGGGTTCCAGTACGAGTTTTTGGAGTCGA TCCTCCACTTCCATAAAAACCTGAAAGGACTGCATCTCCAGGAGCCTGAGTATGTGCTCATGGCTGCCAC GGCCCTCTTCTCCCCTGACAGACCCGGAGTTACCCAAAGAGAAGAGATAGATCAGCTACAAGAGGAGATG GCGCTGATTCTGAACAACCACATTATGGAACAACAGTCTCGGCTCCAAAGTCGGTTTCTGTATGCAAAGC TGATGGGCCTGCTGGCTGACCTCCGGAGTATAAACAATGCATACTCCTATGAACTTCAGCGCTTGGAGGA ACTGTCTGCTATGACGCCGCTGCTCGGGGAGATTTGCAGTTGAGGCCCAGGCTTGCATCCTTTCCCCAGA CCCCCAGGGATACACTGGCCTGGAAAGGGTACAGCGCTGGACCCCACACGGGAACCAGCAGGAAGGAGCT TGGGAGTGGCAATGAAATGCTGAACAGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30315989 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30315989 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12308 same embryo
	EMAGE:12305 same embryo
	EMAGE:12307 same embryo
	EMAGE:12306 same embryo
	EMAGE:12310 same embryo
	EurExpress:euxassay_006978 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source