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EMAGE:12525

Ffar2 free fatty acid receptor 2 ( MGI:2441731)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525
"Pseudo-wholemount" of euxassay_011368. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011368_01 euxassay_011368_02 euxassay_011368_03 euxassay_011368_04
EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525
euxassay_011368_05 euxassay_011368_06 euxassay_011368_07 euxassay_011368_08 euxassay_011368_09
EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525
euxassay_011368_10 euxassay_011368_11 euxassay_011368_12 euxassay_011368_13 euxassay_011368_14
EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525
euxassay_011368_15 euxassay_011368_16 euxassay_011368_17 euxassay_011368_18 euxassay_011368_19
EMAGE:12525 EMAGE:12525 EMAGE:12525 EMAGE:12525
euxassay_011368_20 euxassay_011368_21 euxassay_011368_22 euxassay_011368_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12525Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12525_wholemount_strong.wlz
12525_wholemount_moderate.wlz
12525_wholemount_weak.wlz
12525_wholemount_possible.wlz
12525_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12525_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30156
Entity Detected:Ffar2, free fatty acid receptor 2 ( MGI:2441731)
Sequence:sense strand is shown

>T30156
AATGACCCCAGACTGGCACAGTTCCTTGATCCTCACGGCCTACATCCTCATCTTTCTTACTGGGCTCCCT
GCCAACCTGCTGGCCCTGCGGGCCTTCATGGGCCGGGTTCGCCAGCCTCAGCCTGCCCCTGTGCACATCC
TCCTGCTTAATCTGACCCTGGCGGACTTGCTCCTGTTGCTGCTGCTGCCCTTCCGGATCGTGGAAGCAGC
ATCCAACTTCCGCTGGTACCTACCAAAGATCGTGTGCGCGCTGACAGGCTTCGGCTTCTACAGCAGCATC
TACTGCAGCACGTGGCTGCTGGCGGGCATCAGCATGGAACGCTACCTGGGAGTGGCCTTCCCGGTGCAGT
ACAAGTTATCCCGCCGGCCACTGTATGGAGTGATCGCTGCTCTGGTGGCCTGGATCATGTCCTTTGGCCA
CTGCACCATCGTCATCATCGTTCAGTACCTGAACTCAACTGAGCAGGTGGGCACTGAGAACCAAATAACC
TGCTACGAGAACTTCACCCAAGAGCAGCTGGATGTGGTACTGCCCGTACGACTGGAGCTGTGCCTGGTCC
TGTTTTTCGTTCCCATGGCAGTCACCATCTTCTGTTATTGGCGCTTCGTGTGGATCATGCTCACGCAGCC
CCACGTTGGGGCTCAGAGGCGACGCCGGGCAGTGGGCCTGGCTGTTGTGACGCTTCTTAATTTCCTGGTG
TGCTTTGGACCCTACAACATGTCCCACCTGGTGGGGTTCTACCTGAGGCAGAGCCCCTCGTGGCGGGTGG
AGGCTGTGGTGTTCAGTTCCCTCAATGCCAGCCTGGATCCATTATTGTTCTACTTCTCCTCCTCCGTGGT
GCGCAGAGCTTTTGGGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218874), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 23011. Forward Primer - name:023011_F_IRAV32-35_N19_Gpr43, sequence:AATGACCCCAGACTGGCA; Reverse Primer - name:023011_R_SP6_IRAV32-35_N19_Gpr43, sequence:TTTCCCAAAAGCTCTGCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12526 same embryo
 EMAGE:12529 same embryo
 EMAGE:12528 same embryo
 EMAGE:12524 same embryo
 EMAGE:12527 same embryo
 EurExpress:euxassay_011368 same experiment
 MGI:4824833 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS