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EMAGE:12527

Nrbf2 nuclear receptor binding factor 2 ( MGI:1354950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527
"Pseudo-wholemount" of euxassay_011436. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011436_01 euxassay_011436_02 euxassay_011436_03 euxassay_011436_04
EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527
euxassay_011436_05 euxassay_011436_06 euxassay_011436_07 euxassay_011436_08 euxassay_011436_09
EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527
euxassay_011436_10 euxassay_011436_11 euxassay_011436_12 euxassay_011436_13 euxassay_011436_14
EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527
euxassay_011436_15 euxassay_011436_16 euxassay_011436_17 euxassay_011436_18 euxassay_011436_19
EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527 EMAGE:12527
euxassay_011436_20 euxassay_011436_21 euxassay_011436_22 euxassay_011436_23 euxassay_011436_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12527Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12527_wholemount_strong.wlz
12527_wholemount_moderate.wlz
12527_wholemount_weak.wlz
12527_wholemount_possible.wlz
12527_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12527_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30154
Entity Detected:Nrbf2, nuclear receptor binding factor 2 ( MGI:1354950)
Sequence:sense strand is shown

>T30154
AAGCCATGAAGCTGACCGAGTCTGAGCAGGCTCACTTATCACTGGAACTGCAAAGGGACAGCCACATGAA
ACAGCTGCTTCTCATCCAGGAGAGGTGGAAGAGAGCAAAGCGAGAGGAGCGGTTGAAAGCGCAGCAGAGC
ACGGAACGGGACGGAGCCCCCCACCTGCAGGCACCTCCCAGGCCCTCTGAGGATGCTGAAGGCCAGAGCC
CTCTGCTTTCTCAGCCATACATCCCTTCCACAGAGAGGCGCCTCCCCGAGGTGCAGGGGGTCTTTGACAG
GGATCCAGACACATTGCTGTTTTTACTTCAGCAAAAGAATGAGCCATCAGAGCCCTGTATTGGAAGCAAA
GCCCCAAAAGATGATAAAACGATTATAGAGGAGCAGGCAACCAAAATAGCAGATTTGAAGAGGCATGTGG
AGTTCCTTGTGGCTGAGAATGAAAGATTAAGGAAAGAAAATAAACAGCTAAAGGCTGAAAAGGCCAGACT
CCTGAAAGGCACAGCTGAGAAGGAACTGGACGTCGATGCAGACTTTGTAGAGAAGTCAGAGCTGTGGGGC
TTACCTTCACACTCGGAATCCGCCGCTGCCTCTTCAACCTGGCAGAAGTTTGCAGCTAACACGGGGAAAG
CCAAGGACATTCCAATACCCAACCTTCCTCCTCTGGATTTCCCATCTCCAGAGCTTCCTCTGATGGAGCT
CTCGGAGGACATTCTGAAAGGATTCATGAATGACTAAGATGGAAGGCCATTGGAGAGGCTGGCGACAGTT
AATCCAGAAAAGAATATAAAAGGGAAAGCCACGCCCCAGGGGAATGCCAGAGATGCTCCTTCATCTGGTA
TGCTGTGGGAGAAGAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4212132), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22946. Forward Primer - name:022946_F_IRAV32-35_L19_Nrbf2, sequence:AAGCCATGAAGCTGACCG; Reverse Primer - name:022946_R_SP6_IRAV32-35_L19_Nrbf2, sequence:GCCTCTTCTCCCACAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12526 same embryo
 EMAGE:12529 same embryo
 EMAGE:12525 same embryo
 EMAGE:12528 same embryo
 EMAGE:12524 same embryo
 EurExpress:euxassay_011436 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS