EMAGE mouse gene expression spatial database

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EMAGE:12749

Map2k4 mitogen-activated protein kinase kinase 4 ( MGI:1346869)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011532. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011532_01	euxassay_011532_02	euxassay_011532_03	euxassay_011532_04

euxassay_011532_05	euxassay_011532_06	euxassay_011532_07	euxassay_011532_08	euxassay_011532_09

euxassay_011532_10	euxassay_011532_11	euxassay_011532_12	euxassay_011532_13	euxassay_011532_14

euxassay_011532_15	euxassay_011532_16	euxassay_011532_17	euxassay_011532_18	euxassay_011532_19

euxassay_011532_20	euxassay_011532_21	euxassay_011532_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12749_wholemount_strong.wlz

12749_wholemount_moderate.wlz

12749_wholemount_weak.wlz

12749_wholemount_possible.wlz

12749_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12749_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 13 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 10 11 17 18

pons mantle layer

moderate

regional

moderate expression: see section 08 09 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 10 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 11

ventral grey horn

moderate

regional

moderate expression: see section 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30306
Entity Detected:	Map2k4, mitogen-activated protein kinase kinase 4 ( MGI:1346869)
Sequence:	sense strand is shown >T30306 GGCCGTATATGGCACCTGAAAGAATAGACCCAAGTGCATCAAGACAAGGGTATGATGTCCGCTCTGATGT CTGGAGTTTGGGGATCACATTGTACGAGTTGGCCACAGGCCGATTTCCTTATCCAAAGTGGAATAGTGTA TTTGATCAGCTAACACAAGTGGTGAAAGGAGACCCTCCGCAGCTGAGTAATTCTGAAGAAAGGGAGTTCT CCCCCAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAGGATGAATCCAAAAGGCCAAAGTATAAAGA GCTTCTGAAACATCCCTTTATTTTGATGTATGAAGAACGTACTGTAGAGGTCGCATGCTATGTTTGTAAA ATCCTGGATCAGATGCCAGCCACTCCCAGCTCGCCCATGTATGTCGACTGATACCGCTGCTACATCAGAC TCTAGAAAAAAGGGCTGAGGGGAAGCAAGACGTAAAGAGTTTTCATCCTGTATCACAGTGTTTTTATTGC TCGCCCAGACACCATGTGCAATAAGACTGGTGTTCGTTTCCATCATGTCTGTATACTTCTGTCATCTAGA CTGTGCATCCCTGTAATACCTGACTGATCACAGTGTTAGTGCTGGTCAGAGAGACCTCATCCTGCTCTTT TGTGATGAACATATTCATGACGCGTGGAAGTCAGTACGATCAAGTTGTTGACTGTGATTAGATCACACCT TAATTCATTTCTAGACTCAAAACCTGGAGACGCAGCTACTGGAATGGTGTTTTGTGAGACTTCCAAGGCT GGAAAGACACAGTCATGGATGTACTGTCTGAGCATAGAGAACCCGGGCTTGAGTGAGAAGAGCTTGCACA GCCAGTGAGACACATTGCCTTCTGCAGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3962163), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29463. Forward Primer - name:029463_F_IRAV77-80_J13_Map2k4, sequence:GGCCGTATATGGCACCTG; Reverse Primer - name:029463_R_SP6_IRAV77-80_J13_Map2k4, sequence:CCAGCTGCAGAAGGCAAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12753 same embryo
	EMAGE:12751 same embryo
	EMAGE:12748 same embryo
	EMAGE:12750 same embryo
	EMAGE:12752 same embryo
	EurExpress:euxassay_011532 same experiment
	MGI:4826078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source