EMAGE mouse gene expression spatial database

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EMAGE:12811

Osgin2 oxidative stress induced growth inhibitor family member 2 ( MGI:2384798)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013625. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013625_01	euxassay_013625_02	euxassay_013625_03	euxassay_013625_04

euxassay_013625_05	euxassay_013625_06	euxassay_013625_07	euxassay_013625_08	euxassay_013625_09

euxassay_013625_10	euxassay_013625_11	euxassay_013625_12	euxassay_013625_13	euxassay_013625_14

euxassay_013625_15	euxassay_013625_16	euxassay_013625_17	euxassay_013625_18	euxassay_013625_19

euxassay_013625_20	euxassay_013625_21	euxassay_013625_22	euxassay_013625_23	euxassay_013625_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12811_wholemount_strong.wlz

12811_wholemount_moderate.wlz

12811_wholemount_weak.wlz

12811_wholemount_possible.wlz

12811_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12811_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 18 19 weak expression: see section 10 11 12

trigeminal v ganglion

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24

dorsal root ganglion

moderate

single cell

moderate expression: see section 13 14 18 19 20 21 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6597
Entity Detected:	Osgin2, oxidative stress induced growth inhibitor family member 2 ( MGI:2384798)
Sequence:	sense strand is shown >T6597 AACATGTTTTATATAATCCAAATCTGTATATAAGTAAGATGTGCTTGCTTTCAAAAGGTAATACTGCTTC ATTGTGCTTTTTTCAACTGTTTTAAACTAGAATATGGAAGGTTCTATGTTGACAATCAGCTTTGGAAATT GGATGGAGCTTCCTGGACTTCAATTTAAAGACTGGATATCTAGCAAACGAAGGAACTTAAAGGGGGACCG TGTCATGCCGGAGGAAATAGCTCGCTACTACAAGCACTACGTAAAGGTCATGGGCCTTCAGAAGAATTTT CGAGAGAATACTTACATAACCTCTGTATCAAGACTCTATAGAGACCAAGGTGATAACGGTAGTCAAGACA GAGATATTTCCACAAAGCATTTACAGAACCAGAAGTCAAAATTTATCAAGAGAAACTGGGAAATCAGAGG CTATCAGCGAATAGCAGGTGGTTCTCATGTTCCCTTTTGCCTCTTTGCTGAGAATGTAGCTCTTGCAACT GGAACTTTGGATTCTCCTGCCCATCTGGAAGTTGAAGGGGAAGAGTTTCCGTTTGTGTTTCATTCGATGC CTGAGTTTGGAGCTGCTATAAACAAAGGAAAGCTGTGTGGCAGAGTGGACCCAGTGCTGATTGTAGGTTC CGGCCTTACTGCCGCTGATGCAGTGCTCTGTGCCTATAACAATAATATCCCTGTGATCCACGTGTTTCGC AGACGAGTAACTGATCCAAGCTTAATTTTCAAACAGCTTCCCAAAAAGCTGTATCCTGAGTACCACAAAG TCTACCATATGATGTGTAGTCAGTCCTACTCTGCAGACTCAGGTCCTCTGTCTGATTATACCAGCTTTCC TGAGCACCGTGTGCTTTCCTTTAAGGCAGACATGAAATGCATTCTTCAAAGTGTCTCTGGATTGAAGAAA ATATTTAAGCTGTCTGCAGCTGTAATCTTGATAGGTTCTCATCCTAACCTATCTTTTCTAAAGGAACAAG GGTGTTACCTAGGCCGTAACTCAAGCCAGCCAATAACATGCAAGGGTAACCCTGTGGAGGTTGATGCATA CACCTATGAATGTGTTAAAGAAGCCAATCTTTTTGCTTTGGGCCCTTTGGTTGGAGATAATTTCGTTCGA TTCTTAAAGGGAGGGGCATTGGGTGTTACTCGCTGTTTGGCTACAAGACAGAAGAAAAAGCAGCATTTGT TTGTTCAAAGAGGAGGAGGAGATGGGGTAGCTTAAAGTTCACAAGGAATTTAAATGGACACTTTACTATT AAAGAGTTTTTTAACAGTGGTTTGCAGTGTATTGGCTTGAATTAACTGGGAGAGCCTCCAGCTGTCACTC TTCAAGTTAGCAAAGCTTGGTATCCCAAGTTGTATTCAAGTGTGTCAAAGGTGTCACTCTCAGATAAACC CAAACACTGCCAACTTGGTATAATTTATGCTTTCATTTAACTAGAACATTCTTTTCTAACTTGGCATTTA TAAAGCCAGTTATTCAGACAGAAATCATACTTAATTCATACTGGGTCACCTTACTGCTCGAGATGGTCCA AGGGACAAGAGCATAAGCTTCCTCTGTAGCTCATTAGGCATTTAGACTTAACTCCTCTTGCACAGATCTC CAAGGGTTATGCGTGTAGTCCCTGACGTTTGAAGTACACTCTCTAAATGAAAATGGGCTATAAATATGTT TGAGTAGGATAGGAGGAAGCATTTGCAACTCAGGTTTTATTTATTTTGAAACTATCAGTTATATAAACCT AATTTATTATCAAATATGATGGTCATTAAAAATATTTTTACAAGTTGAGATTTTGACAGATAGGTACTTG ATATCTTTAACTTCTCATAATTTAATATTTCAATAATGCTGACATCCAAGAACCCACTAAGATACTTTTT AGAGATTTATTCTGTTTAGCCATCATAACAGCTAATGAATCTGATTTGTAATTCAATAAATTGTGGATGG AACTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3595088 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3595088 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12813 same embryo
	EMAGE:12812 same embryo
	EurExpress:euxassay_013625 same experiment
	MGI:4826996 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source