EMAGE mouse gene expression spatial database

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EMAGE:12813

Cd2 CD2 antigen ( MGI:88320)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013609. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013609_01	euxassay_013609_02	euxassay_013609_03	euxassay_013609_04

euxassay_013609_05	euxassay_013609_06	euxassay_013609_07	euxassay_013609_08	euxassay_013609_09

euxassay_013609_10	euxassay_013609_11	euxassay_013609_12	euxassay_013609_13	euxassay_013609_14

euxassay_013609_15	euxassay_013609_16	euxassay_013609_17	euxassay_013609_18	euxassay_013609_19

euxassay_013609_20	euxassay_013609_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12813_wholemount_strong.wlz

12813_wholemount_moderate.wlz

12813_wholemount_weak.wlz

12813_wholemount_possible.wlz

12813_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12813_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 04 06 07 08 09 10 11 12 19 20 21 weak expression: see section 13 16 18

ventral grey horn

moderate

regional

moderate expression: see section 15 weak expression: see section 13 14

lung

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8096
Entity Detected:	Cd2, CD2 antigen ( MGI:88320)
Sequence:	sense strand is shown >T8096 AAGAGGTAATCCCAGAGTCACCCCTGGGAAAAGAACTCTAAAGATGAAATGTAAATTCCTGGGTAGCTTC TTTCTGCTCTTCAGCCTTTCCGGCAAAGGGGCGGACTGCAGAGACAATGAGACCATCTGGGGTGTCTTGG GTCATGGCATCACCCTGAACATCCCCAACTTTCAAATGACTGATGATATTGATGAGGTGCGATGGGTAAG GAGGGGCACCCTGGTCGCAGAGTTTAAAAGGAAGAAGCCACCTTTTTTGATATCAGAAACGTATGAGGTC TTAGCAAACGGATCCCTGAAGATAAAGAAGCCGATGATGAGAAACGACAGTGGCACCTATAATGTAATGG TGTATGGCACAAATGGGATGACTAGGCTGGAGAAGGACCTGGACGTGAGGATTCTGGAGAGGGTCTCAAA GCCCATGATCCACTGGGAATGCCCCAACACAACCCTGACCTGTGCGGTCTTGCAAGGGACAGATTTTGAA CTGAAGCTGTATCAAGGGGAAACACTACTCAATAGTCTCCCCCAGAAGAACATGAGTTACCAGTGGACCA ACCTGAACGCACCATTCAAGTGTGAGGCGATAAACCCGGTCAGCAAGGAGTCTAAGATGGAAGTTGTTAA CTGTCCAGAGAAAGGTCTGTCCTTCTATGTCACAGTGGGGGTCGGTGCAGGAGGACTCCTCTTGGTGCTC TTGGTGGCGCTTTTTATTTTCTGTATCTGCAAGAGGAGAAAACGGAACAGGAGGAGAAAAGATGAAGAGC TGGAAATAAAAGCTTCCAGAACAAGCACTGTGGAAAGGGGCCCCAAGCCGCACTCAACCCCAGCCGCAGC AGCGCAGAATTCAGTGGCGCTCCAAGCTCCTCCTCCACCTGGCCATCACCTCCAGACACCTGGCCATCGT CCCTTGCCTCCAGGCCACCGTACCCGTGAGCACCAGCAGAAGAAGAGACCTCCTCCATCAGGCACACAGA TTCACCAGCAGAAAGGCCCTCCTTTACCCAGACCCCGAGTTCAGCCAAAACCTCCCTGTGGGAGTGGAGA TGGTGTTTCACTGCCGCCCCCTAATTAAGAAGGCAGAGTTCGTCATTTCCAATAAAAAGCTGTGTGGATT TATCTTCTTCAGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AGG
Notes:	The probe template was PCR amplified from IMAGE:30077134 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30077134 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12812 same embryo
	EMAGE:12811 same embryo
	EurExpress:euxassay_013609 same experiment
	MGI:4823719 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source