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EMAGE:12854

Grpel1 GrpE-like 1, mitochondrial ( MGI:1334417)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854
"Pseudo-wholemount" of euxassay_011633. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011633_01 euxassay_011633_02 euxassay_011633_03 euxassay_011633_04
EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854
euxassay_011633_05 euxassay_011633_06 euxassay_011633_07 euxassay_011633_08 euxassay_011633_09
EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854
euxassay_011633_10 euxassay_011633_11 euxassay_011633_12 euxassay_011633_13 euxassay_011633_14
EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854
euxassay_011633_15 euxassay_011633_16 euxassay_011633_17 euxassay_011633_18 euxassay_011633_19
EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854 EMAGE:12854
euxassay_011633_20 euxassay_011633_21 euxassay_011633_22 euxassay_011633_23 euxassay_011633_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12854Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12854_wholemount_strong.wlz
12854_wholemount_moderate.wlz
12854_wholemount_weak.wlz
12854_wholemount_possible.wlz
12854_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12854_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
weak weak
regionalweak expression: see section 09 10 11 12 18 19 20
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 13 14 15 weak expression: see section 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19 20
pancreas
weak weak
regionalweak expression: see section 11 12 13 14
thyroid gland
weak weak
regionalweak expression: see section 11 12 16
vibrissa
moderate moderate
regionalmoderate expression: see section 21 22 23 weak expression: see section 06 07 08
midbrain ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
midgut
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
mandible
moderate moderate
regionalmoderate expression: see section 22 23 24 weak expression: see section 03 04 05 06 07 08 09 10 18 19 20 21
lower jaw incisor
weak weak
regionalweak expression: see section 11 12 16 17
maxilla
moderate moderate
regionalmoderate expression: see section 22 weak expression: see section 06 07 08 09 18 19 20 21
upper jaw incisor
weak weak
regionalweak expression: see section 11 12 13 16 17
liver lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 17 18 19 21 22 23 weak expression: see section 12 16 20 24
renal cortex
weak weak
regionalweak expression: see section 09 10 11 12 19 20 21 22
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 04 05 06 22 23 weak expression: see section 02 03 24
clavicle
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 11 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31655
Entity Detected:Grpel1, GrpE-like 1, mitochondrial ( MGI:1334417)
Sequence:sense strand is shown

>T31655
GGCTTTGGCACTGTCGTTCAGGCCTTCTCCTCGCTTGTTGTGCACAGCTACGAAACAGAAGAACAATGGC
CAGAACCTGGACGAGGACTTGGGTCATTGTGAGCCAAAGACAGATCCACCCTCTGCAGACAAGACCCTCC
TGGAAGAGAAGGCAAAGCTGGAAGAGCAGCTGAGGGAGACCATGGAAAAATACAAACGTGCTTTGGCAGA
TACTGAGAATCTACGGCAGAGAAGCCAGAAGCTGGTAGAGGAGGCCAAGTTATATGGCATCCAGGGTTTC
TGCAAGGACTTGCTGGAGGTTGCAGACATCCTGGAGAAGGCAACCCAGAGTGTTCCAAAGGAGGAGATCA
GCAACAACAACCCTCACCTGAAGAGTCTTTATGAAGGGCTCGTGATGACTGAAGTCCAGATTCAGAAGGT
GTTCACAAAACACGGCTTGCTCAGGCTTGACCCAATTGGGGCCAAGTTCGACCCTTACGAACATGAGGCC
TTGTTCCACACCCCTGTGGAGGGGAAAGAACCAGGCACTGTGGCACTAGTTAGTAAGGTGGGCTACAAGC
TGCACGGACGCACCCTGAGGCCAGCTTTGGTGGGGGTGGTGAAGGACGCTTAGCTCCCTCCCTCAGGCTC
TGGACTTTGTAGGTCACTTGCTAGAACTCGAAGGGCGGGCTTGTAATTTCTCATCTGTGAATTCATCTGA
CCCTTGCCCAGCCTTGTTGGAAATCTTAAGTAAGCTAAGCAGAACATGAAGCTGCTTGCACAGTCAGGGA
CTCTGGGAGTCTGGTCACTGAATTTAGCATTACCTACTTCAGAAGAGGGCCAGCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3589725), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14802. Forward Primer - name:014802_F_IRAV08-11_K21_Grpel1, sequence:GGCTTTGGCACTGTCGTT; Reverse Primer - name:014802_R_SP6_IRAV08-11_K21_Grpel1, sequence:CTGCTGGCCCTCTTCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12857 same embryo
 EMAGE:12855 same embryo
 EMAGE:12856 same embryo
 EMAGE:12859 same embryo
 EMAGE:12858 same embryo
 EurExpress:euxassay_011633 same experiment
 MGI:4825244 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS