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EMAGE:12857

BC030336 cDNA sequence BC030336 ( MGI:2446240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857
"Pseudo-wholemount" of euxassay_011621. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011621_01 euxassay_011621_02 euxassay_011621_03 euxassay_011621_04
EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857
euxassay_011621_05 euxassay_011621_06 euxassay_011621_07 euxassay_011621_08 euxassay_011621_09
EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857
euxassay_011621_10 euxassay_011621_11 euxassay_011621_12 euxassay_011621_13 euxassay_011621_14
EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857
euxassay_011621_15 euxassay_011621_16 euxassay_011621_17 euxassay_011621_18 euxassay_011621_19
EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857 EMAGE:12857
euxassay_011621_20 euxassay_011621_21 euxassay_011621_22 euxassay_011621_23 euxassay_011621_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12857Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12857_wholemount_strong.wlz
12857_wholemount_moderate.wlz
12857_wholemount_weak.wlz
12857_wholemount_possible.wlz
12857_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12857_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31682
Entity Detected:BC030336, cDNA sequence BC030336 ( MGI:2446240)
Sequence:sense strand is shown

>T31682
ACCCGGACTGGATCAACACCGGGGAGTCGGCGGGAGCACTCACTGTGGGCCTGGTGCGGCAGTGTCAGAC
AATCCATGGACGAGACCGGACCTGCATCCCTCCTCGGCTTCCCCCAGAATGGGTCACCACCCTGTTTTTT
ATCATCATGGGAATCATTTCCTTGACTGTCACATGTGGTTTGCTGGTGGCTTCCCACTGGCGAAGAGAAG
CTACAAAATATGCTCGATGGATAGCATTCACTGGAATGATCCTTTTCTGTATGGCTGCCCTAATATTTCC
AATAGGATTTTACATCAATGAAGTCGGAGGTCAACCTTATAAGTTACCCAACAACACAGTAGTTGGGTCA
TCATATGTACTTTTTGTCTTATCGATTTTCTTCACAATAGTAGGACTTCTGTTTGCTGGCAAAGTCTGTT
TACCAGGCTGACGAATGTCTAAACGGCTTAACTCTTTTTATTTTTTTATTTTATTTTTTATTTTTAGAGG
TTGGGAGGACAAAGAGAAGGCATCTGGGCAGTCCTCTCATAGGAAGATGCTTAGATGAACCTGATGCTGA
AAAGGAAAAATTGCTTTGATTTGTTCTCACTATGCACTTTGGATTTAAAAAAAAGAATGAGAGCCTTTTC
CATAACCAAATACAATATTGACTAAATCTCCTGAGCATATTCAGGCAGTCAGTCTGCACTGTGATAGCAA
CCTAGTGAAGGAGAGTGCTTTATGCTGAAAGCATGTGGCCCCTTGTGACTCTGTTGATCTGTTTTTAATG
TCTAATGATTCATTTGAGAGGGGGGAAAGTCTAGTATTTATGAATCATGATGGACCAGAAGGGTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5401060), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 32529. Forward Primer - name:032529_F_IRAV81-84_E09_BC030336, sequence:ACCCGGACTGGATCAACA; Reverse Primer - name:032529_R_SP6_IRAV81-84_E09_BC030336, sequence:TGCACCCTTCTGGTCCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12854 same embryo
 EMAGE:12855 same embryo
 EMAGE:12856 same embryo
 EMAGE:12859 same embryo
 EMAGE:12858 same embryo
 EurExpress:euxassay_011621 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS