EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12920

BC030307 cDNA sequence BC030307 ( MGI:2387653)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013695. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013695_01	euxassay_013695_02	euxassay_013695_03	euxassay_013695_04

euxassay_013695_05	euxassay_013695_06	euxassay_013695_07	euxassay_013695_08	euxassay_013695_09

euxassay_013695_10	euxassay_013695_11	euxassay_013695_12	euxassay_013695_13	euxassay_013695_14

euxassay_013695_15	euxassay_013695_16	euxassay_013695_17	euxassay_013695_18	euxassay_013695_19

euxassay_013695_20	euxassay_013695_21	euxassay_013695_22	euxassay_013695_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12920_wholemount_strong.wlz

12920_wholemount_moderate.wlz

12920_wholemount_weak.wlz

12920_wholemount_possible.wlz

12920_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12920_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 13 14

pons mantle layer

weak

regional

weak expression: see section 06 13 17

midbrain mantle layer

weak

regional

weak expression: see section 15 17

facial vii ganglion

weak

single cell

weak expression: see section 03 07 19 21

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 06 07 17 18

trigeminal v ganglion

weak

single cell

weak expression: see section 02 03 06 07 17 18 19 21 22

vestibulocochlear viii ganglion

weak

single cell

weak expression: see section 07

ventral grey horn

weak

single cell

weak expression: see section 10 11 14 15

dorsal root ganglion

moderate

single cell

moderate expression: see section 17 18 weak expression: see section 08 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31675
Entity Detected:	BC030307, cDNA sequence BC030307 ( MGI:2387653)
Sequence:	sense strand is shown >T31675 CCATTGCATCCCTTCCACAAGAAACAATTTAAAGAAGTGATTTGAATGAAACTGACCAATTGCTTATAGC AAACACAGTTAAAAGAAGACCCAAACTGTGGACCTTGGCGACTGGGCCAGGATGGAACTTCCTTTCTTCT AAGTGCGACATCATCCTGGAGCCGAGTGAAATGAGCGACGAGGCAAGTGAGACCGGACAAAGATACAACG GTCAACCCATTTTAAAGCGCCAGAAACCCATCCTACCTTACATATGTTCCACTCTAGATTTTCAAGAAGA GAGAGACTTTTTGGCCAAAAGCATCTTCCCTCGGCTTAATGACATCTGCAGCTCCCGGGGCACCTACTTC AAAGCTGTGGACTTGAGATGGTCAGCTGTGAAGGCCCATAAGTCCTTCACCAGCAACCAGTTCCGACAGT ACTCCTGTCTCCAGTCTCAACACCTGAAACTCTCCCTGGACTACGTGAACAGATGCTTCCCGTTTTTCAT CGGCCTGCTGGGGCAGACCTACGGAGATTTCCTCCCCGACTACACACCTTTCCTGTTATCCCAAGTGAAG GACTTCGAAAGTTTATCCAAGGGAGAACAGAATCTATACATTGCTGCCAAAAATGGTTACCCTTGGGTTC TCAAGACTCCCAACTGCAGCCTGACAGAGTTCGAGATCATCCAAGCAGTATTCCGGAAGAAATCTCAATT TCAATTTTTCTACTTCCGGACATCAAATTCGCTGCTGCGAACTTTTAATGAGGAAGAGGAGGAGGAAGAG GAGAAGCTGTCCTCAGCATATCTGTTGAACGAACAGGGGAAGATGAAGGTTGGAAAGCTCAAGGCTAAGA TCATTGGCAAAGGGCTTCCTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5369121), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 32734. Forward Primer - name:032734_F_IRAV81-84_C05_BC030307, sequence:CCATTGCATCCCTTCCAC; Reverse Primer - name:032734_R_SP6_IRAV81-84_C05_BC030307, sequence:TGACAGGAAGCCCTTTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12922 same embryo
	EMAGE:12921 same embryo
	EMAGE:12924 same embryo
	EMAGE:12919 same embryo
	EMAGE:12923 same embryo
	EurExpress:euxassay_013695 same experiment
	MGI:4823421 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source