Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12958

Rars arginyl-tRNA synthetase ( MGI:1914297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958
"Pseudo-wholemount" of euxassay_013703. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013703_01 euxassay_013703_02 euxassay_013703_03 euxassay_013703_04
EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958
euxassay_013703_05 euxassay_013703_06 euxassay_013703_07 euxassay_013703_08 euxassay_013703_09
EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958
euxassay_013703_10 euxassay_013703_11 euxassay_013703_12 euxassay_013703_13 euxassay_013703_14
EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958 EMAGE:12958
euxassay_013703_15 euxassay_013703_16 euxassay_013703_17 euxassay_013703_18 euxassay_013703_19
EMAGE:12958 EMAGE:12958 EMAGE:12958
euxassay_013703_20 euxassay_013703_21 euxassay_013703_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12958Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12958_wholemount_strong.wlz
12958_wholemount_moderate.wlz
12958_wholemount_weak.wlz
12958_wholemount_possible.wlz
12958_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12958_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32000
Entity Detected:Rars, arginyl-tRNA synthetase ( MGI:1914297)
Sequence:sense strand is shown

>T32000
ATGGAGTCCAGCTGCCTGTGCTTGGAGACAAGGAGAAGGTTATAGTTGACTTTTCCTCTCCCAACATAGC
TAAGGAGATGCATGTTGGCCACCTCAGGTCAACCATCATAGGAGAGAGCATGAGCCGCCTCTTTGAATTT
GCAGGATACGATGTGCTAAGGTTAAACCATGTAGGAGATTGGGGAACCCAGTTTGGCATGCTCATCGCTC
ACCTGCAAGATAAATTTCCGGATTATCTGACAGTTTCACCTCCTATTGGGGATCTGCAGGCCTTTTATAA
GGAATCTAAGAAGAGGTTTGATGCTGATGAGGAGTTTAAGAAGCGAGCCTATCAGTGTGTCGTTTTGCTG
CAGAGTAAAAACCCAGATATCATGAAAGCCTGGAACCTCATCTGTGACGTGTCCCGGGAAGAGTTTAAGA
AAATCTATGATGCATTGGACATCACTTTAATAGAGAGAGGAGAATCCTTCTATCAAGATAGGATGAAGGA
CATTGTAAAGGAATTTGAAGATAAAGGGTTTGTGCAAGTGGATGATGGCAGAAAAATTGTGTTCGTGCCT
GGGTGTTCTGTGCCATTAACGATAGTGAAATCAGATGGGGGCTATACCTATGACACATCTGACCTGGCTG
CCATCAAACAGAGACTGTTTGAAGAAAAAGCAAACAAGATTATTTATGTGGTGGACAATGGACAAGCTAT
ACACTTCCAGACAATATTTGCTGCTGCTCAGATGATTGGCTGGTATGACCCGAAAGTAACTCTAGTGACC
CACGTGGGATTTGGTGTCGTCCTAGGAGAAGACAAGAAGAAATTTAAAACACGTTCAGGTGAAACTGTGC
GCCTTATGGACCTGCTGGAAGAAGGGCTGAAACGGTCCATGGACAAGCTAAAGGAGAAAGAGAGAGACAA
GGTCTTAACTGAAGAGGAATTGAAAGCAGCTCAGACATCCGTTGCATATGGCTGCATCAAATACGCCGAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3989952), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22056. Forward Primer - name:022056_F_IRAV32-35_E04_Rars, sequence:ATGGAGTCCAGCTGCCTG; Reverse Primer - name:022056_R_SP6_IRAV32-35_E04_Rars, sequence:GGTCGGCGTATTTGATGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12960 same embryo
 EMAGE:12959 same embryo
 EMAGE:12961 same embryo
 EMAGE:12957 same embryo
 EurExpress:euxassay_013703 same experiment
 MGI:4827618 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS