Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12959

D730039F16Rik RIKEN cDNA D730039F16 gene ( MGI:1925246)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959
"Pseudo-wholemount" of euxassay_013645. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013645_01 euxassay_013645_02 euxassay_013645_03 euxassay_013645_04
EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959
euxassay_013645_05 euxassay_013645_06 euxassay_013645_07 euxassay_013645_08 euxassay_013645_09
EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959
euxassay_013645_10 euxassay_013645_11 euxassay_013645_12 euxassay_013645_13 euxassay_013645_14
EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959 EMAGE:12959
euxassay_013645_15 euxassay_013645_16 euxassay_013645_17 euxassay_013645_18 euxassay_013645_19
EMAGE:12959 EMAGE:12959
euxassay_013645_20 euxassay_013645_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12959Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12959_wholemount_strong.wlz
12959_wholemount_moderate.wlz
12959_wholemount_weak.wlz
12959_wholemount_possible.wlz
12959_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12959_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
renal cortex
weak weak
regionalweak expression: see section 07 08 09 10 11 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32005
Entity Detected:D730039F16Rik, RIKEN cDNA D730039F16 gene ( MGI:1925246)
Sequence:sense strand is shown

>T32005
GGACAGACCGGAATCACGGTGCCCTCCACCGGGCTCCCTCAGGCTCGTCCTTACATGCCTCCTGATCCTG
ACAGCTGTTCTGATGTATCCCGTGCTCAGGACCTTTAGCCTCTGGCTCCACTCTTCCCTCACTGGGATCT
ATGTCTCTGGCTCTTATTCCATCGTTTTGTCAACTGTCCCAATGAGCAAATTGCCAGAGACATCGCCAGG
GCCATCCTGGATAAGAAGATGGCTTCGTCTGTGAACATCTTGCCGAAGACCTCTTCTCTGTACTTTTGGA
AAGGAGAAATAGAAGAAGGCATTGAGGTCTCACTGGTTGGCGCATCCTTTTGAAATCCCAGAGGTCTTCA
GTATTCCCATGGACCAAGGAGATGCTCGCTTTTTACGATGGCTTGAGGAGGGAATGAAGGAGAACTAAGG
AGAGAGAACCTTATTGTGCAGGCTTCTGCCTCTAGCCTTTGTTGACTGTGTTCTGGGAAAAAGCAGGCCT
CCTTCTGCCTCCCATTGAATGTTCCTAACAGCAGACAGGTTCTAGCTGACCTGTGCATGCTGAATGAAGG
GCTGAGAATGTCAAGGCTGGAAGTGAAAGCCAATCAGGCCTCCAAGAACCAAGTGGAACCGGTTTCGTGT
GTTTGCAGGTGGCCTGAGGGTACGTGGGTAAGGATGAGAGACTGAGGGGAGGCAGCAGTGACGGTGAGGT
TTCTCGTCTTCAGGCTCTGGGCTGACCATAAAGTACCTAGGGTATGATTGTTCCTCTGTTTCTTGTGGGG
ATAGGTGACCTTGATCATCTAGGGGTGTGTGGGAGAGCTACTTTCTAAAACAATTGATATGGGCTCTATT
CAAAACAATGAACAGTCCTGGTACGATTAGAGGTCAACAGAATTGATTCTTGCTTTTGAATCTTACAGAG
GTGAAGCTAGAAAAGGGGTGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4194825), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22831. Forward Primer - name:022831_F_IRAV32-35_H11_D730039F16Rik, sequence:GGACAGACCGGAATCACG; Reverse Primer - name:022831_R_SP6_IRAV32-35_H11_D730039F1, sequence:GGGCACCCCTTTTCTAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12960 same embryo
 EMAGE:12961 same embryo
 EMAGE:12957 same embryo
 EMAGE:12958 same embryo
 EurExpress:euxassay_013645 same experiment
 MGI:4824209 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS