EMAGE mouse gene expression spatial database

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EMAGE:13026

Eif2s2 eukaryotic translation initiation factor 2, subunit 2 (beta) ( MGI:1914454)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_013689_01	euxassay_013689_02	euxassay_013689_03	euxassay_013689_04	euxassay_013689_05

euxassay_013689_06	euxassay_013689_07	euxassay_013689_08	euxassay_013689_09	euxassay_013689_10

euxassay_013689_11	euxassay_013689_12	euxassay_013689_13	euxassay_013689_14	euxassay_013689_15

euxassay_013689_16	euxassay_013689_17	euxassay_013689_18	euxassay_013689_19	euxassay_013689_20

euxassay_013689_21	euxassay_013689_22	euxassay_013689_23	euxassay_013689_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13026_wholemount_strong.wlz

13026_wholemount_moderate.wlz

13026_wholemount_weak.wlz

13026_wholemount_possible.wlz

13026_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13026_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6580
Entity Detected:	Eif2s2, eukaryotic translation initiation factor 2, subunit 2 (beta) ( MGI:1914454)
Sequence:	sense strand is shown >T6580 GGGAGCTACGCTGACTCCGGGCCGTGAAGCCGACCGGTTTGACTTTTCATCCCTGCCGAACCCGCTCGAA CTACAGCCATGTCCGGGGACGAGATGATTTTTGATCCTACTATGAGCAAAAAGAAAAAGAAGAAGAAGAA GCCTTTTATGTTAGATGAAGAAGGTGATGCCCAGACAGAAGAAACCCAGCCCTCAGAGACAAAAGAAGTG GAGCCAGAACCAACTGAAGAAAAAGACGTGGACGCTGATGAGGAAGACAGTAGGAAGAAAGATGCTTCTG ATGACTTAGATGATTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAGACAAAAAAGATATTTGACAT TGATGAAGCTGAAGAAGCTATAAAGGATGTTAAGATTGAGAGTGATGCTCAAGAGCCAGCCGAGCCAGAG GATGACCTTGACATTATGCTTGGCAACAAAAAAAAGAAAAAGAAGAATGTTAAATTCCCAGAAGAAGATG AAATACTAGAAAAAGACGAAGCTTTAGAAGATGAAGACAGCAAAAAAGATGATGGCATTTCATTCAGTAG CCAAACTGCTTGGGCAGGCTCAGAAAGAGACTACACATATGAGGAGTTGCTGAACCGAGTGTTCAACATC ATGAGAGAAAAGAATCCAGATATGGTTGCTGGAGAGAAGAGGAAATTTGTTATGAAACCTCCACAGGTCG TCCGAGTAGGAACCAAGAAAACTTCTTTTGTCAATTTTACAGATATCTGTAAACTATTACATCGTCAACC CAAACATCTCCTTGCATTTTTATTGGCAGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTT GTAATCAAAGGAAGATTCCAACAGAAACAAATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCA CTTGTCACACATGCCGGTCACCGGACACAATCCTACAGAAGGACACCCGACTCTATTTCTTACAATGTGA AACTTGTCATTCTCGATGCTCTGTTGCCAGTATCAAAACTGGCTTCCAGGCTGTCACAGGCAAGCGAGCA CAGCTCCGTGCCAAAGCTAACTAATTTGCTAATCACCATTGATTTTTCCAAAATGTGTTGGAGATTTGGC TGGACAGGTTACCATCAGAGTGGATGTACCATTGAGTTAAAAACAAGATAGAAAAACTGCCAAGTTGTTT GGCAAGTGATTGTTCTGAAATACCTTGCAAGATACCGATATTCAAGCTGTTGACATACTCGTTGCCTACT TTAACAACTGTCAGAGAAACGTGATGGGGGTAAGGAGGTACTTTTTAAAATCGTTCATAGACTTCTGTAA AATGCAAGATAAATTAAAGTTATTATAACAGTAATTCTTTCAAGTCGATCTGTCCTTCAGTTTTCTTTTC TACAATAAAGTGTTCGTTAAATTTGGTTAAAAACAAAACAACAAACTTGATTCCTGGGCTGTGGCAAGTT TTCTTTGTCCTCAAAACTGGCAGATACTGGAGCTGGGCAGCCATTTTTCCTTTTAGAGTCTCTTGAAGCT ACATGGCTAGAATAGATAGGTTTTGGAGGGTTTAATTTCCTTTTGTGAGGATTACTGCTACTTCTTCCTA GCCAATTCCTCATTCTCTCACGTGACAACAGCTGAAAGTGTCAGGTGCTAGATTCCTCCTCCTTGGAATT GTGTTAGGCTAGTTGGTCCTCTTTGAGGAAGCATATATATAGTACAGACTTCTTAGCACTTAGGAAGCTT CTCCAACCTGTGCTCTAGGTCAGTAAAACTTCGTCCTCTGAAATGCTTTTCTGTACTAAACTAACTTGAA CGTGACCTAGAAACCAAGAGATACTGCTTCATGTGTGGTCTTTATTGGGCCACAGGAGGGTCAATCTTGT GGGTCTGTGCTGTAATCTGCCTTACTTGCACCCACCAGGATAAGGACATAAACGGTGCTCAACTTTTCTG CCACCAGACTGTTAACTTTGCTAAATGGCTCAAGAACAGTCAGTTAGCCCTGCTATCTTGACTATGTTGT TTTTAATCACTGTGACCTCTGAATTTTCAAGTATAGGCCAAATGAACCAAGTAACTTGGCTGTGAAATGG CAGAAGGAACCTTCTCTCAAGCAGGTAGGTCAAGAGATGGGCCATATATATACTGGTTGACCTGCCATCA CCGCTCACTGCCATGTGAATAGAACTGTTTATGACTGCAATTTCTTTTTTCTTTTTCTTTTTTTTTTTGT ACCTCTGCCCATGATGCCCAATGTTGGCTATAACTGCGTGAATAAAGCAAGGATCAGTGCCTCCTGTGTA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3594058 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3594058 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13027 same embryo
	EMAGE:13029 same embryo
	EMAGE:13028 same embryo
	EurExpress:euxassay_013689 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source