EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13027

Narf nuclear prelamin A recognition factor ( MGI:1914858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013636. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013636_01	euxassay_013636_02	euxassay_013636_03	euxassay_013636_04

euxassay_013636_05	euxassay_013636_06	euxassay_013636_07	euxassay_013636_08	euxassay_013636_09

euxassay_013636_10	euxassay_013636_11	euxassay_013636_12	euxassay_013636_13	euxassay_013636_14

euxassay_013636_15	euxassay_013636_16	euxassay_013636_17	euxassay_013636_18	euxassay_013636_19

euxassay_013636_20	euxassay_013636_21	euxassay_013636_22	euxassay_013636_23	euxassay_013636_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13027_wholemount_strong.wlz

13027_wholemount_moderate.wlz

13027_wholemount_weak.wlz

13027_wholemount_possible.wlz

13027_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13027_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24 moderate expression: see section 03

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19 20

trigeminal v nerve

strong

regional

strong expression: see section 10 11 18

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 15 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 24

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4253
Entity Detected:	Narf, nuclear prelamin A recognition factor ( MGI:1914858)
Sequence:	sense strand is shown >T4253 CGTCGGCCTCCTGCCAGTCCCACTCCAGATGAAGTGTGAGCACTGCACACGAAAGGAATGTAGTAAAAAA TCAAAAACTGATGACCAGGAGAATGTGTCCAGTGATGGGGCCCAGCCAAGCGATGGGGCCAGCCCAGCCA AGGAGAGTGAAGAGAAGGGAGAGTTCCATAAATTGGCTGATGCAAAAATATTTCTGAGTGACTGCCTGGC ATGTGACAGCTGTGTGACTGTGGAGGAAGGTGTCCAGCTTTCTCAGCAGAGTGCCAAGGACTTCCTTCAT GTCCTGAATCTTAATAAGAGATGTGATACCTCAAAGCACAGAGTTCTGGTTGTGTCTGTGTGTCCTCAGT CTCTACCTTATTTTGCTGCTAAATTCAACCTCAGTGTCACTGATGCATCTAGAAGGCTGTGTGGCTTCCT CAAAAGTCTTGGGGTGCACTATGTATTCGACACCACAATCGCTGCAGATTTCAGCATTCTGGAGAGTCAG AAGGAGTTTGTACGCCGGTATCACCAACACAGTGAGGAACAGCGTGAACTGCCCATGTTGACCTCTGCCT GTCCTGGTTGGGTCAGATATGCTGAGCGGGTGCTGGGTCGCCCCATCATCCCATATCTCTGTACCGCCAA GTCCCCCCAGCAGGTCATGGGCTCCTTGGTGAAGGATTACTTTGCCAGGCAGCAGAATCTGTCTCCAGAG AAAATCTTCCATGTTGTCGTGGCTCCTTGCTATGATAAGAAGCTGGAAGCTCTTCGAGAGGGACTTTCCA CTACTTTAAATGGTGCTCGGGGCACTGACTGTGTGCTGACATCAGGTGAAATTGCTCAGATAATGGAGCA AAGTGACCTCTCTGTGAAAGACATTGCTGTGGATACTTTGTTTGGAGACATGAAGGAGGTGGCAGTCCAG CGTCATGATGGAGTGAGTTCAGATGGGCATCTGGCTCACGTTTTCAGACACGCAGCCAAGGAGCTGTTTG GCGAGCACGTGGAGGAGATCACCTACCGTGCACTCAGGAACAAAGACTTCCATGAGGTTACCCTTGAGAA GAACGGGGAGGTCCTACTGCGCTTTGCTGCAGCGTACGGCTTTCGCAACATTCAGAACATGATCCAGAAG CTCAAGAAGGGCAAACTCCCATACCACTTTGTGGAGGTGCTCGCGTGTCCCAGAGGTTGCTTAAATGGCA GAGGCCAAGCACAGACAGAGGACGGCCACACAGATCGTGCACTGCTGCAGCAAATGGAAGGGATCTACTC CGGCATCCCTGTGCGGCCCCCAGAGAGCAGTACACATGTGCAGGAGTTGTACCAGGAGTGGCTGGAGGGT ACTGAATCTCCCAAAGTCCAGGAGGTGCTGCACACCAGCTACCAGAGCTTGGAGCCCTGCACAGACGGCC TGGATATCAAGTGGTGACAAAGCCTGAAGGGCAGGAGGGGACAGCTCTGAGTGGAGAGTGGTGGGTGCTT CAAGGAACAACAGGTCCCTACAGCTTTGCCAGCGCTTCAGGACACAGTTTTCATGTGGTGCTATCTTTGT AGTGTGTGAGGGAGTGGAATGTTTGCACATGGGAGAGGTATTTTCCCTCTCTGCGTAGCCTTTCATCCCA AGATTGTGAGAAAGATCCCAAAGAGGCCAGGGACAGATCCCTGATTTGCAGGTTTAAAAAAAAAAATCAA GATGTGTTTTTAGAGAACCAAGACCCAGGGGTTGTGATCCACGAGCCTAAGATTATTGCTGCAGAAATGG AGGCATCAGGAAGGCCAGGCTGCTGTACAGTGGATAACTATGGCCTTCAGAGAGGCCCAGTCTTCTGGCC CAATTTGGCTCTTTCATTTGTATACATTTTTTTAAAAATAATTTTGAATTCTAGGGCCAGCAAGATGGCT TATTGGTACCACCATTAGTTTGATCCATGGAGCCCACATGGTGGAAGGAGAGACCAACGCCTACAAGCTA TCCTCTGACTTTCACACAGGCACATGGCATGCATATGTACCACCGAGACCACACCCCGAAAATAACCGTA AAGAAAATTACACTAGTTTTAAGTCATAGTAACAGTGAGCCATATTTTTTATAGTCCTTGAAGGCCGGCA GTCATTACAGCCCTGGGCCACTGGTGTGCCTGAGTCATTCTTTTACTTGACCTGCCATCAGCGTTGAGCT GGACTCACCTCAGAAGAAAGGCTGCTGAGAATGGCATGCATCACACCCTGCATTTGATGCAATGTATATT TAAAAATAAACAAGTAGAGAATTAAGAGGGTGGCCCACCTAGGCTTTGGGGTTCTAAGGGTGGTGCAGCT CATAGTTACTGCTGTGGGGCCGGAAGACAGAGCTGAGGCTCCGTGGACCCTGGAATGGGTGAACCAGCTT TGTAATGTCCAGCCTCCTCTAAACTCCTCCCCAGTGCCTTTTCATACAAATAGGCCTGGAGCACCTCCTC TCCTCCACAAAGCCCACTGGTCTGGGATAGCATCCCTTCAGCCCTCACTATGCTAATGCGAGCATGCCTT AGGGGAAACAGTATGTCCACACACAGAAATAGCTGCCCGATGGTGAAATTCACAAAATCATGTGACACAT ACTAGATAGAGTCCACCCAGGAGATAAAGGGGTTTGATGAAGCCCCTGAATTTTGCTCAGTGTGTGACTG CCTTGACCCTTAGTACTGTCAGCTCTATCACAGTCCCACATGCCTCATGTCTTGGTCTTCCTGTGAGTCT GTCTAGGAGTGTAACTGGATATGTTATTGTAAACCAGTGGTTCTCAACTTGTGGGCTGTGTCCCCACAAA GGGGTCACATATCAGATATTTACATTATAATTCATAACAGTAGCAATACTACAGTTATGAAGTAGCAACA GAATAATTTTATAATTGTTGGGGGGGTCACCAGAACAGAAGAAACTATTAAAGGGTCACAGCATAGGAAG GTTGAGAACCACTACTATAAATTATGATTTTTTTTTTTTTTTTGCTTTCAGTCTATATGCCTTGGGTATC CATCCCCAAAGTAACTGGTTATGGAATAAACAATGCAATGGGGTCTTGAGTGTGGCATTGAAGGAGTCAG CTGTTCTCCTGTCCTGGACTACACCCCGCAGTCCGCCTCCCTCACCAGGCCCCAGCCTGGGTGATGATAC CTTTTTCCCAGTCCCTCTCTAATAGGTCAGGAGAAGGTTCAGAACACGGGGAGTCAGCGTGGGAAGGGAC TGACTTCCTGCAAACTTCATGGCACCACCCATGATAGCAACATAGGCTAGGGGCTAAGATGTGAGATCTA TGACCTTCTGTTTCACTGATGCTAGGCCGTGGCCCTGTGAGCTTTTCAAGACTACAGGTGCCTTTCTGTC CCAGCACTTGGAGCCAGAGGCAGGTGGATCTCTGTGAATTAGAGGCTAGTCTAGGTTACAGAGTGAGTTC CAGGACAGTCAGGAATACATAGAGCAACCCTGCCTTATCCCCCAACTCCCTCCCCCCTAAAAACAAAGCC CAAAGCAATGGAAGTATGAAGTGAGCAAGTTCTTGACAGACAGGACCCAGTCAAAGCATCACGACAGTCA GAGTGGACAACGTACGCTGGTGCAGGCATGAGGGGCATAACCCAAGCTCCTATCTGCCCAGTGCTCTGTT GTACCATGCTTCTGCTCTGCTTGGGCAGAGGCCCAATCTTAGTTTGATTCCTACCTGGCCCGAGCTGTAT GGGTTTGCACTACTGTGGGTAACCGTGCTTCTACATGATGAGATTCTGTCTCAAAGACTAAATTTGGTTT CTGTTACAGGTAATTTAGAAATAGTTCTGTCAGGGCTTGTGCCATAGTTCAGGAGCAGGGCGTGTGCCTA GCATCCATGGGCACCACATTCGTTCTCAAGATCCACAGTAGCAGAAAGTGACAGGGAGGATTGCTGCATT CTTTAGTACGTTGTTTGGACTGCTGTTAAGATCTGACTCAGTGTGTGCCTTACCCATTACTGGTCGACAT GTGGGCTTCCCAGGTGCATCACAGGTGAGCCAACCACAGGGTTTGGGGTTGCAGCTGGCAGCTAGGCTGC AGGCGCTGCCCTGGGTACACCACTCACCTGAGGGGCACAGCTGGCCGGAAGGGTAATCAGGAAGTGTGGC TCCTTTTCCACTTGGCATATTACATATGGGGCTCCCTCCTGCATCTAGAGATGGGAAACTGCCTGAAGAA CTTACTCTGGTTTACCATTTGAGAGAGTGCAGTCATTTATGTCAGAGAAGACACCTCGCATCTCACTACT CATGAAGCACCGTCTCTTCTGTGGATTCACGGCTCAGCTTGTAAACATTGAGTTGAAATGGAAGATTTAT TATGATTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503713 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13029 same embryo
	EMAGE:13028 same embryo
	EMAGE:13026 same embryo
	EurExpress:euxassay_013636 same experiment
	MGI:4826598 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source