EMAGE mouse gene expression spatial database

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EMAGE:13033

Ap3s2 adaptor-related protein complex 3, sigma 2 subunit ( MGI:1337060)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013638. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013638_01	euxassay_013638_02	euxassay_013638_03	euxassay_013638_04

euxassay_013638_05	euxassay_013638_06	euxassay_013638_07	euxassay_013638_08	euxassay_013638_09

euxassay_013638_10	euxassay_013638_11	euxassay_013638_12	euxassay_013638_13	euxassay_013638_14

euxassay_013638_15	euxassay_013638_16	euxassay_013638_17	euxassay_013638_18	euxassay_013638_19

euxassay_013638_20	euxassay_013638_21	euxassay_013638_22	euxassay_013638_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13033_wholemount_strong.wlz

13033_wholemount_moderate.wlz

13033_wholemount_weak.wlz

13033_wholemount_possible.wlz

13033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

pons mantle layer

weak

regional

weak expression: see section 07 17 18

facial vii ganglion

weak

regional

weak expression: see section 04 05 06 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 09 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 09 18 19

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 15 16 17 18 19

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 15 16 17

vomeronasal organ

weak

regional

weak expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9362
Entity Detected:	Ap3s2, adaptor-related protein complex 3, sigma 2 subunit ( MGI:1337060)
Sequence:	sense strand is shown >T9362 CCGAGAGAAGCGGGACCGGCAAAGATGATTCAGGCGATTCTGGTTTTCAACAACCATGGAAAACCGCGGC TGGTCCGCTTCTACCAGCGTTTTCCAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTGGT CCTCAAGCGAGATGACAACATCTGTAACTTCTTAGAAGGTGGAAGTTTGATTGGTGGCTCTGACTACAAA CTGATTTACCGGCACTATGCCACCCTCTACTTTGTGTTTTGTGTGGATTCATCAGAGAGTGAACTTGGGA TCTTGGACCTCATCCAGGTTTTTGTGGAGACACTGGATAAGTGTTTTGAGAATTTATGTGAGTTGGACTT GATCTTCCATATGGATAAGGTGCACTACATCCTGCAGGAGGTGGTGATGGGTGGAATGGTGTTGGAGACA AACATGAATGAAATTGTGGCTCAAATCGAAGCTCAAAACCGACTGGAGAAGTCGGAGGGCGGCCTCTCTG CAGCTCCAGCAAGGGCCGTGTCCGCTGTGAAGAACATCAACCTCCCTGAGATCCCCAGGAACATCAACAT CGGTGACCTCAACATCAAAGTCCCCAACCTGTCCCAGTTTGTGTGAAGGTGGGGGACTGGGCTGAACTGG AGTTCGCAGGATGAGCAAAGCAGTCAAGTCCAGAAGCAGAACCCCGTTGAGCCTTTAGAGTCGCACCTTG GGGCCCTGCACCTTTCCCAGAGTGGGAATGGAGTTGGGTGCACACTTCCTGGCCATCCTGCTGCTCCTGA CTCACGGCTGTGATAGACATCAGGGCACTAAGCCACTGCTCCTTCGCCTCCCCCACACAGCTGCCCTCAG GGCCAGGGACTGTGAACGCGCCACCTGCTTCCCAGGGCCCCACCACTGGGACTGAACACATCTGACTTCC TCTCTAGCACCAAGGGCTTCTGAGATCCTGTATCACTTACTGGGGGAAAGACCAGGGGGAAAGACTAGCC TTTGAGCTGTCAGGCATGGTAAGTGGCAGGTGAGGGCAGGGTGTACACCACCTCTGCTAGGGCCTCTATA CCCTCTGAATGGTCAAGCATTGTGGTCCGCAGTTGCTGCAGAGCAGTTTCCTAGTATATACCTAGTCTTG TTGTCAGAGGCATCTGTGCGCTGGATGGTTTAAGTCACACTCTGTGTGCGTGCCACTACCACCCCCCCCC CCCACACACACACACACAGAGTTGGTGCGACCCCCAGAACCCTCTCGCAGAGGAGACCAATGTCTGTAGA CTGTGGCGACTGGATCCCTCCTAGGTTATGACTTTTCCCAAGTTTAAAATACACTGTTCTCTTGGAAGCA GAAGGTTCAGTGCTGTGCTGTGAATGAAGAAGTCCCCACATACACACACTTGCTCAGAATCCACTAACCA GTCTCAGATTCTAGAGCTGGGAGCCTGGGCTGGGTGCTATATGGGCTGAGCCTGCAGCATGCTAGCTGTA GTGCTGTGTGCTTCCCAGCTTGAGTCCTGCAGTGAACACACTGGGCTCCCTTGGTTGACATTCTACAGCT CTGGAACCCTGCAAAGTTGCATGTGCTGGTGTGGAGGCTGATGCACCGCCATGGGGAACCGCCCCACGCA GGCCCCTCTCCCTCCCTCCCGATAGGATAGTGTATTTCCATGGCTTGTGATGCTGATGTCCTAGGAGACC GTGTGTCTTACCCAGTGTGTCTCAGTCCCCAGCAGGGCTTCCTGCACTCTGCCGGTGCTGATGCAGCATC CTGTCCTCACCTTTCAGTCCTCTGTCAGAGCTGCAGTGATGGTGGGCACTTGGCTAGAGGACAGACCTGA GATCAATCCTACTGCATTCTCTGGAAGCATCTCATTGCTATTTTATGTTATTTTATTGCCATTTGTGTAC ATTCATCTCCTTTTACAGTGGCTGGAATCAAGCATTTGCTCTAGGGGGACATGAAGAGTCCTTTAATAGT CCTTACTAGAGACATTTTTTTAAGCTTTATTTTATTTCTGGGGTGTGTCTGTCTGTCTGTCTGTCTGATG GTATGCTCTGAGGCCAGAGGAGAGTATACATGCCCTCTGAGGCTAGAGAAGGGTATGGAATTCCTTGGAC CTCAAATTAACAGGAGGTTGAGAAGCCCCCTAAAATTGTGGTGCTGGGAAGCAGACTCAGGTCCTCTGCA AGAGCAGCAGTGCTCTGAACTGGAGCCACCTCTCTGGCCCCACCCATGGACATTTTACTGCCTGGTTAAG CAGACTTTTGTCACCCAGGGCTAAGGTTGGAAACGTGCCTGCCTGGCTCCAAAGCTCTTTTTTTTTCTCC TTACTATTCAAGATAGTCTCTCCCAGTTTTACAACCCTCTCTTTTATAGATGACAGACAGGGAAACTGAG ACTTCAGGGGATTGAGCAATCTCACAAACTAATAAAGACAGGCTTAGTTCTCTAAGGTAGGCAGAGGAGA GTGGAGGATCCTGTGTCCCTTCAGTTAATTGCCTGAGATGGAGCCGCCCAGAGTGCTGTCAGGCTGAGGG TCAAAAGGGCCACAGTGCTGCTCTAAAGTATGCTTGCTGTTGGCCTAAGGCACCATTGAGTTGGAAGCAC TTGGCTTCCCAGGTAGTTTAGATTTCAGGTGTTTGCTTGCTAGAAACTACTTGGTTCCCCAAAACTGTGT CCTGGTACTGTAGACTGCAAAAGCAAGCATCACACAACGGCTTGCACCATGTCCCCTGACAGTCTGCTGG TTCAGGCTTGGAAAACTCAATTCCTCTAGTCAAGCCAGAGGGATTAATTCATTGCTAATGACTTGCATCT ACTTTGTCTGCTATTAAGAAAGCCATTGTCTGAGATTCTCATTCTCTCTCTCTCTCTCTCTCTCTCTCTC TCTCTCCCCCCTCCCTCCTTCCTTCCCTCCTTTCCTTCCTCTTTCCCTCTCCCCCCTTTGTGCTTTGAGA CAAGATCTCACTTTGTAACCTAAATTCTCAATCCTCCTGCATCACCTCCAAAATGCTGAGATTACAAGTG CCCTGTGCACTTTGCTGTGACATAGAGGAAGTCCTTGGTCCTTCTGGAGAGGGGTGTTCCTTTTGCTCTT GAGACATCAGTGACGCAACCAGAGGCCAGGGTTGTGTTTGCCTTGGGGTCCTGGACCTGGGTGGTTACAC AGGGGCTTCCCTGGGTGTTCTCACTGACTCTAGTTAACAGCTGGAACCCCCCACTCCCTGTCTTCTGTGT AACTACTCTTGGGTCTAAACTAAGAGAGAGGCTGTCTTTGCTCCACTGTCTTTGACCATCCCTGACAGTG CTCTGTAGTGACAATTCTGGAACTTTGATTTTTTTTTTTTAAAAGAAAGAAATAAGAATACATTTTTGGT TTACTCCCAGGCGTGGGGTTTTGGGTCTACTTCACAGCAGCTGACCATGACTTACCTTATGCTCTAGCAG ATGGTTTCTGCGATTGTGTGACGTTTGGAACTTTTTAGAGGGTATATACTGCTAGGGCCCAGATAGGTGG GATCAGTGGTTGTTGGTTGTTGGGGGTTGGGGGGGTGGTTGGTTATAGTTTTTAGTAGTTTGTGTTCAAA GAAGAAACAGAAAGAAATTTGATTCTGAGATCTCTATTTCTCTCTCTCCCACCTCTATCCTCTCTCTTCT ATCTAATGATAGGGGGTGAAAATGAGGGGCTAAAGGGTAGGGAAAAGAAGGACCCACAAAAGTAGCAAAG ACCAGCTATAAGCGAAAAGAAACAAGAAGGAATTAGATTCAGAGATCTCTATCTTTCTCCCCTCTCTCTG TTCTCTCCTATCTAGTGATAGAGGTAAAACTTGGGGGGGGGGGGGTCGTGTAAAGGGTGGGAAAAGAACT CACAATATAGCAAGGCCCAGCTACAGTGCTTACGTGGTTGGAACATGGGCACTGACTGGCAGCCTGGCAC CATGGAGGCCAGACTCTGACTGCAGTGTGGAAGATTTAAAGGACAGTCCCCACCCCTGACTTGCCAGTGT CATGCCTACTGCATACAGTGCTGGACAAATTACCTTTCCCAAGCCTGACCATGGCACCATACATGCTACA CTGAGGGTAGCGAGTCTCCTAGCCCTGATGTTTCGTCCTCTGCCAGATGCAGAGCCAGCCCTCTCTCCCT CCCTCCCTCACCACTGCAGAGCCAGTTTAGCAATTCTTTCTGTGACCTCAGCTTCAGAAAAGAAATTAGG AGCAATGCCTCTGCCTAGGTCCCAGACACGATCCCTGGCCTGGCCTGGCCTGTAAAGGCTTTCCTCAGCA TGGCCACATCAGCCATGGGTACCTGGAAATGAAGCTCCAGAGCAGGTCCCTGCCCTCCTTCACAGTGACC AAGAGGAGTACTGAGCCCCACCTGCTCTGCACACATCCTGGATGCCCACTCTTCCAAGTGTGCTGACCAC CCAGAGTGAGCCAGCTCTTTGTGGGGGCTGCCAGGCTAGGCCCAAACCTCAAGGTCACAGTAAGCCTTGA CCTTTTGGGAAGCTGTGGGGTGTTCTTCACCTGCATGTTCTGGCAGATGGGGCTGTTGAAATACCTCAAT GCAAGTGATTAACCCTTTCCAGAGCTCTTTCTTATTTCAACTCTTCCTTTCCAAATAAGAACTTAATTTA TGTAGTTCGGGTTTTGAGGGGTTTGTTTTGTTTAGTTATTCTGTTTTGTTTTGATTTTCAAGACAAGATA TCTCTGTAGCCCTGGCTGTCCTAGAACTTGCTCCATAGACCAGGCTGGCCTTGAACTCATAGATCTGCCT CTGGGATTAAAGGCATGCAACACCAACATCTGGCTATAGGTCAGTATTTTTACATGGAGGTGGAGAAGTA CCCTCAGAGACCAGACGAAGGCCTTGGATCTCTTGGAGCTGGAGTTGAATGGGGGTACTCTGAACTGCTG AGCCGTCTCTCCAGCCCCATGCACTTTACATGTTAAGAAAACAAAAAACCAGTTTAGCAGAGGATAGCCT ATTCCCAGCCACTGAGCACTGCCCTCCTGGCAGCAAGTGTTTGCAGTTTGAAGAAGAAACAAGCGTCCTT TATCTTCACTTTCAAAACTAAGGTCATAGCTTCAGTTGCCCTCAGCTCAGTACAGTCTTACTGTCCTCCA GAAATGCAGAGTCCACCCTACACATACACCTGTGAGCACTCACACCAAGAAGTCCATCCTTACAGTCCCA CGGTACCTCTGCGTTCTAGGACTGTAGGACTGTGACATGGGAGTAGGGAGGCCCATGGGTGCAGGTGGAG GACAGCAAGGATTGTACCGAGAAAAGGATGAGATGATGGGCATGTTCGCTTGCTTGGGGATAAACGCCTG CTGGTGAGTGAGTGGACAGGAGAGCGTGGGTCCAGCTCTGTCACGTATGGAGTTGGAGTAAGTGACATGG CCAAGGGATGAGAGTAAAGGACAGCCTGGGCAAGGGCTGAGGAGTGTGCACATTGGAGCAACCTGACGTC CGGGGAGAGCAGAACAGGAAACACACTGGTCAGACAGGAAGGGACCCCAGAGCCACAGCTCTAATCCTGC CTGGAAGCTGTATATGAGGGTATGAGCAGAGCTGGAGAGACACTGCTCGCTCACCTCATGGACGGGTCAT CTGCTCCTCCTCCTGTCCCACATCCCCAGCTTCACCATGAATCCTTTAACAAGCCCTGAACTTAACCTAA GCCATTCACGGCGTCTCTTTTCTAGGACCTGTTCCTGCTCTACAGCTTGGCCTCCTAAACTATCAGAAAC TATAATAAAACTTCTAAAAACCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853219 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853219 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13032 same embryo
	EMAGE:13031 same embryo
	EMAGE:13030 same embryo
	EurExpress:euxassay_013638 same experiment
	MGI:4823166 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source