EMAGE mouse gene expression spatial database

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EMAGE:13148

Eaf1 ELL associated factor 1 ( MGI:1921677)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017241. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017241_01	euxassay_017241_02	euxassay_017241_03	euxassay_017241_04

euxassay_017241_05	euxassay_017241_06	euxassay_017241_07	euxassay_017241_08	euxassay_017241_09

euxassay_017241_10	euxassay_017241_11	euxassay_017241_12	euxassay_017241_13	euxassay_017241_14

euxassay_017241_15	euxassay_017241_16	euxassay_017241_17	euxassay_017241_18	euxassay_017241_19

euxassay_017241_20	euxassay_017241_21	euxassay_017241_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13148_wholemount_strong.wlz

13148_wholemount_moderate.wlz

13148_wholemount_weak.wlz

13148_wholemount_possible.wlz

13148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9368
Entity Detected:	Eaf1, ELL associated factor 1 ( MGI:1921677)
Sequence:	sense strand is shown >T9368 GCGGAGTAGATTGGTTTCCGGCCCCGGGTGGGCGCGGACTTGGTTCAGTGCGCGCTCCGGAGCGGTGTCC CGGGAGCGCGGTCCTGCGGGCCGCTTGCGCGGGGATCCGGGGGGACTGCGGCCGCGCGAGGAGAGCTCCA ATCCGGGTGCCGGGCAAGTGCAAGGCCATGAACGGCACGGCGAACCCGCTGCTGGACCGGGAGGAACACT GTCTGCGGCTAGGGGAAAGCTTCGAGAAGCGGCCGCGGGCCTCCTTCCACACGATTCGCTACGATTTTAA GCCAGCATCAATAGACACTTCCTGTGAAGGAGAACTACAGGTTGGCAAAGGAGATGAAGTCACCATTACG CTGCCACACATCCCTGGGTCCACGCCGCCAATGACGGTGTTCAAAGGGAACAAGCGGCCCTATCAGAAAG ACTGTGTGCTCATTATTAACCACGACACTGGAGAGTATGTGCTGGAGAAGCTGAGCAGCAGCATTCAGGT CAAGAAGACAAGAGCTGAGGGAAGCAGTAAAATCCAAGCTCGAATGGAGCAGCAGCCCGCTCGTCCCCCA CAGCCGTCACAGCCACCCCCTCCACCTCCACCCATGCCGTTCCGAGCCCCAACGAAGCCTCCAGCTGGAC CCAAAACTTCTCCCCTGAAGGATAACCCCTCACCTGAGCCCCAGTTGGATGACATCAAAAGAGAGCTGAG GGCCGAGGTGGACATTATTGAGCAGATGAGCAGCAGCAGTGGGAGCAGCTCCTCCGACTCTGAGAGCTCC TCGGGAAGTGACGACGACAGCTCCAGCAGCGCAGGCGAAGACAATGGCCCAGCCTCGCCTCCCCAGCCCT CACACCAGCAGCCCTACAACAGCAGGCCTGCGGTGGCCAATGGAACCAGCCGGCCCCAAGGAAGCTCTCA GCTCATGAACACGCTCAGAAATGACCTGCAGCTAAGTGAGTCTGGCAGTGACAGTGATGACTAGCACCAG GCTTTTCAAATCTGCTTTCTGGAACTCAAACATGCTACAAGACCCAGCTCCATCTGCCAGGACCACATGT CACAGGAGGATGTTGCAGATCAGAGCCAATAGGAGTTAAGGCCTAGAAGCACTCAGATACTCAGTCTTTA ACTTGGTGGTGGTGGGTGATTTTCTCATGTAATTTTTGAACAATCTCTTGATTCACAGTTTAAGTATATC TATAGGAGAACTAACAGAAATCTCTTTTATTTGGTTAACATTGTTAATGAAAAGCAGACCAGTGTACCCT GGTCTAGGATACTCAAAATCCATATCTTCATAGGTCAGTGATTGAGCAGCTCAGCTTGCCTGGGTGGTCT CTATCAGTAAAATACAAGTCTTTTAAGGGCATGGAAAGAAAAGAGATCTTCAAATTGTGTCATAGAAATA CATTAAGACCACTTTACAGTGTTGATACTAAGTGGTAAGGAAGTTTGTGACCTAGTTTGACCAGACCATA GAAGTTGACCTTGGCAGAGTCTTTGGGTCAGTTGTGTGTGTGTGTGCTCGAGATCCAATAGCTCAGTCCA GCACAGCTAGGAAAATGGACGCACACAGCAGGGAACTGCCAGTCACTGTCCCTGGGCAAGCTTGCTCACG AATTCCTCTTTTTGAATTACACTTGCTGGCTTCAGATTCTCTGGCCTGGAACTTTCTATTGGATTCTGAA AAAACAAAAGCATTGCTGGCATTCAGCTTGGGTTCAGACTCGGGCAGTTCCAGCTGTGTGTGCGCGTTCA GCTCATCTGAGGTGTTTGCTTTTGTGCAGATCCATCCCTAGGTTCTTCATTCCCAGGATGTTAGCAGAGG GTAGAAATCAGAGGGATGTGGTGAAGAACTTCTGAGCACATTAGCCAGCAGCAAGGCTCCACAGGTGAGA ATCCAGACAGGAGTTGGGCAGTATGGAACAGTCTTTAGAAGCTGTTTGCTTTGTGCTGCTGTTGGTGCCT GACTCCCAGTCTCGTGGGTTATTTTTATAGAAGAGAATCACAAGATTGCCTTTTGCTGTGTGCCATTTCC AAATAAATGTTCTTGCTGGAAACTGGTCACTAGTCAGGGTTGCACATTGAGGAAGTATGTGGTGACTTAT GTGTCTGCCCATCTCTGTGGTCTGAATGAATATATCCTAGGAAATCCCTGTCTTTCTGCTTTAAGACATT AACAATGCTGTTCTTTCCTCCTGCATAGTGGAGGAAGGTGGTCTGGACCTGGTGGGGCCGTAGGTCATAT AGATTAGCTTTATGGTCGACACACCATACAGATCAGCTGATCCACTCAGAGACTGAAGTGAACTGAGATG GGTGGGGCCATTGCCCAGCAGCACACCTACACGTCTCAGCACAAGCTCAGATGCAGAGTGTGCACCCATA CCCTCTGCTCAGTCTCAGCCCACTGGGAGATGCTCTATGGATCCATGGCAGGTGGAACCTATTTAAAGCA AACTGATAATTGCTTTATAATTCTTTGGTAATGCTGGGTGTGGTGCCGCACGCCTTTAATCCCAGCACTC GGGAGGCAGAGGCAGGTGGATTTCTGAGTTCCAGGACAGCCAGGGCCATACAGAGAAACCCTGCCTCGAA AAACCAAAAAACAAACAAACCAACCAACCCTCTTTGGTAATGACAGCTCAGGGAAGGTGAGGGTTGTGAC TGGGTGACTTGAGTTGTCACCAGATGTGGGCATTGAACTGTTTTTACTGCTCTTCGCTGTTCAGACTGTG GCAGGCTCCAGGAACTTGAACTAAAAATGCCTATTTAAGTCTCCCCTTGCCCCGCTTTTCTCCAAAAGCC TTGGTTTGTGTAGACAGAGCTGTGGTGTTGGCATTCCAGTTGGGGACTGTTGCCATAGGAAGTACTTGCA TGTGGCACTGGGCTTGATAAATTGAGCTTTCTGTGCCTCAGACGATCTAGTCACCCGGCTTACCCAGCAC CACAGGTCCTTCTGTAGATGGCTCCAGGTTTTGCTCTCTGCACAAATCCTCCGGTGATTCTTTCTGCTTC CCCCACTAGAGTAAGAAAGCTATTTTTGGAAATCCAGAATGTAGCCTCCCAGATGGCTTCTCAGGGGTAT CCTTGTGCCAACCAAAGGACTCCTTCCTGTCGCTGTGCCTTTGCTGTCCCCTCCTCTTCACCCACTCTAA ACCTCTCTCCAGAGCGCCTATTTCACAAGCTCTTCATTTCTTTCAACCTATGGGTAAGCCCTGGTATATA TACAAACTACCATTCTACCTGGTCTTGGTTAGTATTAGTGCCTCAAAAGCAGGGGCTGTCATCACGGAAC TAGACATGCAGGTGCTCCTACTCTGGTTGGGAAAGAGATGGAGACAACTACTAGTGAAGGGTGGAGGGTA AACTGAAACATTCCAGGCATTGAGACAAAGCTGGAAATCTGTACGCAGTGCCCATGCAGGTCTCTACCGA ATCTGTCAATGACCTTTTTTTAATTTGCTCTATGCAATGTTCACTTGGGCAAGGTGGAAGGACTAATTCT ACAGAGATGCCATGGCAATCTTTGAAAACCATTGGGAAAATGCAATACAGATAAACTAGAGATTCGTATA ATGCCACGTGTTAGCTTGTTTCTGTAGCAGAATAACTCTCTGCGGTGACGTGCTGGAGTGAGAGGGAAAC TTGAAAATTCCAAAGTCCTTCCCTCTTTTGAGAGACTGGTTGTGTGTGTGATATTTATTAATTATTTTAA GAGAGAATTGTTTTGTAGATAGATATGTATTAAGAACAGCCAAACTGGATCTTTCCACTTGGCAAGGAAC AGAAACTTTTTGAAACTCAGGCCTTAACCAGTAGCTTGGAAGACCAACTCAAGTGTTAGGAACTGTGTGT TTTTGTTTCCTGTTATTTCTGTCTTAGATTCTTTGTGTCCTTTAATGATATTTAAAATCTTGTGCCTAAA AATTTATTTTGCTTAATTCTGATGTAGACATGCATGTTTACATTTATATAAAATATTTTCTGTATAGTTT ACTCTCTTTCAAGATGAATATATTTAAATAAAAATGAAGGTCAGCAATATAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6853920 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853920 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13150 same embryo
	EMAGE:13149 same embryo
	EMAGE:13145 same embryo
	EMAGE:13146 same embryo
	EMAGE:13147 same embryo
	EurExpress:euxassay_017241 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source