EMAGE mouse gene expression spatial database

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EMAGE:13150

Cep135 centrosomal protein 135 ( MGI:2681869)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017238_01	euxassay_017238_02	euxassay_017238_03	euxassay_017238_04	euxassay_017238_05

euxassay_017238_06	euxassay_017238_07	euxassay_017238_08	euxassay_017238_09	euxassay_017238_10

euxassay_017238_11	euxassay_017238_12	euxassay_017238_13	euxassay_017238_14	euxassay_017238_15

euxassay_017238_16	euxassay_017238_17	euxassay_017238_18	euxassay_017238_19	euxassay_017238_20

euxassay_017238_21	euxassay_017238_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13150_wholemount_strong.wlz

13150_wholemount_moderate.wlz

13150_wholemount_weak.wlz

13150_wholemount_possible.wlz

13150_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13150_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9360
Entity Detected:	Cep135, centrosomal protein 135 ( MGI:2681869)
Sequence:	sense strand is shown >T9360 CGGCCTTTAAGTTGGGAGTGTGACTAAAACTGGTTTTGGAAGCAAGATGACTACAGCTGCAGAGAGAAAG TATATTAACATTCGGAAGAGGTTAGACCAGCTGGGCTACCGCCAGACCCTGTCAGTGGACAGTCTGCCTT TGGTAGAAAAACTTTTCAGTGACCTCGTTCATACGACAGAAAGCCTGCGGCAGTGCAGGTTGTCCTCGGG AAAGGCGGAAAAGGAAAGTGCCAATCTCGATTTTGTTTTGGAACCTTATAAACTTGAAAATACAAGACTG AATAAGGAGAATAATGAATTGTATCTGGAGTTAATGAAGCTCAGAGAATGCTCAGACAAGCACATTAAAG ACTTGAAGACGACACTGAAGAAATGTTCCCGTGAGACAGCTGATCTGAAGTTTCTGAACAACCAGTATGT TCACAAGGTCAAAGTCCTGGAGAAAGAGAGCAAAGCCAAGGATGAAAAGATCCAGCAGCTTCAAGAGAAG AACCTGCGTGCTGTGGTGCAGACCCCTGGTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGC AAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACAT TGCGGACCTCCTGCAAGTGGCTGACAACAGGATTCAGGAGCTGCAGGAGGAGGTCCAGCAGCTGCAGGAG AAGCTAGCACAGATGGAGAAAGGAGTGCTGGACTACAGCAAGCAGATTGAACTGAGAGAACGAGAAATAC AGCGACTGTCACTTGCGTTGGATGGCGGTTGTTCCCCTGATGTCCTGTCTCTGGAGACCAGAAATAAAAC CAATGAGAAACTCATTGCTCACTTAAATGTCCAGGTTGACTTCCTCCAGCAAGCTAATAAAGAGCTGGAG AAGCATATTCAAGAGCTCATGGAAACCAAGGAAACAGTGACAACTGAAGTTGTGAACCTCAGCAACAGAA ACGAGAAGCTCTGCCAGGAGCTAACCGAGATCGACCAGTTAGCCCAGCGGCTGGAAAGGCACAAGGAGCA AGTGCTGGAGACAGCAGACAAGGAGCTCGGGGAGGCAAAGAAAGAGATTAAACGAAACCTCTGTGAAATG CGGAACCTTGAGGAAAAAATGTCAAAACTGCAGTGGGAATTGGACTTAAGCCATAAGGAGAAGGAGAGAC TGAACAGTGAGCTGCTTTTAAAGTCAGACCTGGAGACTGTTGTTCATCAGCTCGAACAAGAAAAGCAAAG ACTTAGCAAAAAGCTGCAGAGTTTTGCAGTCACAGAAAGAGAACTGACTCTGGAGGTTGAGAGGATGAGG CTAGAACATGGGATAAAGCGTCGGGACAAGTCACCCTCTCGTCTGGACACATTTCTGAAAGGCATAGAGG AAGAGCGCGATTATTACAAGAAAGAGCTGGAGAAACTGCAGCATCTAATCCAGCGGAGATCTTGTGCGAT TAATTACTCCGCTCGGGAAAAACCTCCTGTAGTCAAATGCTCAGAAAAGGGTGACTGCAGCACGGACGTT CACCTGATCACAAGAGAAAGGGATGAACTTCAGCGCATGCTGGAGAGATTCGAGAAATACATGGAGGACA TACAGTCCAACGTGAAGCTGCTGACGGCGGAAAGGGACAAGCTGAATGTCCTGTATAAGGAAGCAAAGGA AGAGTTGTCTACACTAAGGAAGGAGTCCACTAATTCGACATCCCCCAACCATCTTGTTAGTTGTGTAGAA AAGGAGAAAGAACGCGCGTTATCTGAGTTGAGAAGAATTACAGCAGAAAAGGAAGCTCTGAGAGAAAAGT TGAAAAACATCCAAGAAAGGAATGCTGTTGGAAAATCAGACTTAGAGAAAACTATTGAACATTTGACATA TATTAATCACCAGCTTGAAAATGAAAAATATGAATTGCAATCTAAAATGTTAATGATGAAAGAAACAGTG GAGTCATTAGAGAACAAATCAAAACTCCAAGCTCAAAAGCTTAGCCATGTGACTGGTGACTCATCTCATC AGAAAACAGAGATGACCTCCCTTAGGATCGTCAGTGAGCAGCTACAGCGCTCACTTGATGACTGTCAGCA CCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGAGCAAATTAAAATGCTGGAGCAAAAACTA GAGAATCTCAGCCACAGGATGACGGTGCAGAGCGAAGAGACTCACGCCATGAAGAAGACCATTGGAGTGA TGGACAAAGAAAAGGACTTTCTCCAGGAGACTGTGGATGAGAAGACAGAAAAGATCGCCAACCTGCAGGA AAGCCTCCTTAGTAAAGAAAAAGTCATTGCCCAGTTGAAGGTCACAGTTGCAGAGTATGAAACATCACTT AACCAGCTACAAGAAACTTTGACTACTCGAGACCGAGAGATAAACAGCCTCCGGCGCCAGCTTGATGCAT CTCACAAGGAGCTTGATGATGTTGGGAAATCTAGAGAGATCTCTTTTAAGGAAAATAGAAGGTTACAAGA TGACCTGGCCACAATGGCGAGAGAAAATCAGGAGATCTCACTGGAGCTGGAAGCAGCAGTGCAAGAAAAA GAAGAGATGAAGAGCAGAGTCCATAAGTACATCACTGAGGTGTCGCGGTGGGAGAGCCTGATGGCCGCCA AGGAAAAAGAAAACAAAGACTTGCTGGATAGATTCCAGATGCTCCACAGCCGTGCTGAGGACTGGGAGGT CAAGGCTCAGCAAGCGGAAGGGGAGAACAGCTCAGTCCGCCTGGAGCTGCTGTCTATCGACACAGAGAGA AGGCATCTGCGGGAACGAGTGGACCTCCTGGAGAAGGAGATCCAGGAGCACATCAATGCACATCACGCAT ACGAGTCTCAGATCTCGTCAATGGCTAAGGCCATGTCTCAGTTAGAAGAGGAGCTGCGACGTCATGAGAG CGAGAAAGCCACCATGTTAGGTGACGTGTCGTCTCTCCGAGAACTGTGCATCAAGCTCGACTCAGGCAAA GACGTCATGACCCAGCAGCTGAATTCCAAAAGCCTAGAGCTGGAGCGGGCGGTAGCAGAATTAGAGAATG TAAAATCAGAATCAGAACTATTAAAAAAACAGCTGACGAATGAGAGACAGACAATTAAAAACCTTGAGTC GTTGTTAGCTACAAACAGAGATAAAGAGTTCCAGTCCCATTTAACCTCCCACGAGAAGGACACAGAAATC CAGCTGCTGAAGGAGAAGCTAAATCTCTCAGAAAGCAAACTGACTACCCAAAGCCGAGAAACCTCCATGC TTCGAACTAAAGTGACACAGTTGCAGACAGATTATGATAATCTGAAGAGGCAGATGTCAAACGAGAAGTA CGAACGAGAGCGAGCAATCCAAGAGATGCGCCGACTTGGTCTTCCTACATCACCTCTGAGCTCTACTCTG AAATCTCCTGTACAGACTCCTGATCATATAAATGCATAGTTACCAGGAAGATTATGGTTTTCAGTGAGTT TCTGCGTGGATTTTTTAAGAACACAGAGTAATGAGATATTTGAAGTTCTTGTTCCTGCAAATCATGAACA CTGACACAAATTCTACCTCTGTCAACCTTTATTTAAATCAGTGGATATTTATGTAAAAATGTTTAGTTTT AAAAGAGCCTTATATGTATATTTTCATATGTGAAAAAACAAAATATGTATTAATAGCTTAACTGATGTGA TATGTGAATTTTTATTATTCCCAACTAAAATTACTCCTTACAATGTGTTTATACTGTGAATTGCTTTAAC ATGCTCACATTATGCTTTAATAATCTGTGCCTTAGTGAACCATAATATATGCATGGAAGGATTCTTGCAC TATTTAGGTTTCGTGCCTGTGTTTATAGCCCATAGCATGATGAATTCAGAAAATGATCCATTTGATGCTG TTCCGTAAGTGTGACATCAGTCCACGTGTGCTGTCTATCACACAGGGGAGTCTATCTAAATCTAGTCTAA TAGAACTCTTTTTATTTTAAAAGTAAAGAAAGAGTTTATGTGAGCTTCTGTTTCCAGAGAGTTAGGACTC CCTCAGGGAAGCCGAGCGGCTGACAGCGAGCTCACCACACAGAGCCTAGAGTGGCCAGGAAGTAGGGGAA GCTGTGTACTCCGAAAGCCCTCCCCCCAGGGACGCACTTGCTCCAGTAAATCTGCACCTCTTAGACCTCC TCAAACAGCACCCCCACACACCTGGGGCCAAGTGTTCAAGTCTCTGGGTCTGTCTGTGGGATACATTTCT CATTCAGACTACATTTTCTAAAACCAAAAATTCAACTGATACCAATTTTCCCCCCTGAGGTGTTTTTTGC TATTTCTGAAAGTTCAGACCTACTGGAGGTCAATAAACTCAGCTATCTGTTTGCAAATTACTTACATTTT CATCAACTTTGAAAAACATTTTCCTGGCTATAGAGGAAATAAATGTGCCTAGTGATCTCATCTGGATAAA ATAGTTATCGCCGTGTGTGATAGTGCAAGCAGGAAGGGCGGCAGTGGGAGACACTTTTATCTATGTGGGC CTTATTTGGGCTGTCCTATGCGCTCTCCACATGACACATTTTTTTTAAGAATTAAGTTAAAATAATTCAG AGTTTTGTAAAATAATCAAATGAAAATTTTTTTTACTAACTAAATTCTTCAGTGTACTAGTCTAAAATTT CAGAATATGAAAGTATATAAAGTAAATATAGAAAAGTGTTTTGTTAAAAGTCCAATATGCATCTGATAAT ACCTATTTGTGGCTCCAAATCCTATCTAATATCCCCAGTCTTTCCGAATAGTATGTCAACACCATCCCTG TGCCGTATAAATTGAAAACACCTTTTTATAACTTCCCTTTCAAGGTTGTGTAACTCTGAAGAATTGAGGT GTATGCTCAGCCTTAGAAAAGAGTAGACATACAAAATAAAATGTCGACACCAATACTAATCATGTACCAA CTGTTCGCGTTTGAAGAAACCCCTCTTTTATAACTAGATTTCATTATGGTTGAGAAAGAACTTAAAAGTC AGCGCAGGCCAGCGAGTCAGCTGGGAGCAACCCGCTTTGTTTCCTCTTGGCTTAAAGCAAGTTGCTCTCT GTGAACGTCCCTGAGGGTGGGTAGACATCCAGGTAACTAGTGTGCCTTATGTGGTCAGGATGTTCCCTTT GCAATAATTTGCTGCATAGTTGAGTACAGGGCGACTTACTCTCCCACTATGTGTATAATTTATTCATTCT ATCTCCTTTGAGTAAATTCATGTTTTATAGCAAAATATGAATAAAAAGGACCATTTACAATGTCAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6852711 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6852711 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13149 same embryo
	EMAGE:13148 same embryo
	EMAGE:13145 same embryo
	EMAGE:13146 same embryo
	EMAGE:13147 same embryo
	EurExpress:euxassay_017238 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source