EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13404

Epha4 Eph receptor A4 ( MGI:98277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017515. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017515_01	euxassay_017515_02	euxassay_017515_03	euxassay_017515_04

euxassay_017515_05	euxassay_017515_06	euxassay_017515_07	euxassay_017515_08	euxassay_017515_09

euxassay_017515_10	euxassay_017515_11	euxassay_017515_12	euxassay_017515_13	euxassay_017515_14

euxassay_017515_15	euxassay_017515_16	euxassay_017515_17	euxassay_017515_18	euxassay_017515_19

euxassay_017515_20	euxassay_017515_21	euxassay_017515_22	euxassay_017515_23	euxassay_017515_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13404_wholemount_strong.wlz

13404_wholemount_moderate.wlz

13404_wholemount_weak.wlz

13404_wholemount_possible.wlz

13404_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13404_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7912
Entity Detected:	Epha4, Eph receptor A4 ( MGI:98277)
Sequence:	sense strand is shown >T7912 GGCAGGAGCAGCGTTGGCACCGGCGAACCATGGCTGGGATTTTCTATTTCATCCTCTTTTCGTTTCTCTT TGGAATTTGCGACGCTGTCACCGGTTCTAGGGTATACCCCGCGAATGAAGTTACTTTATTGGATTCCAGA TCTGTTCAGGGAGAGCTTGGGTGGATAGCAAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGCATTATGG ATGAGAAAAATACACCGATCCGAACCTACCAGGTGTGCAACGTGATGGAAGCCAGCCAGAACAACTGGCT GCGAACTGACTGGATCACCCGCGAAGGGGCGCAGAGGGTGTACATTGAAATTAAGTTCACTCTAAGGGAC TGCAACAGTCTTCCGGGCGTCATGGGGACTTGCAAGGAGACGTTTAACCTCTACTACTATGAGTCGGACA ACGACAAGGAGCGCTTCATCAGAGAGAGCCAGTTTGGCAAGATCGACACCATCGCAGCTGATGAGAGCTT CACGCAGGTGGACATTGGTGACCGAATCATGAAACTCAACACTGAGATCCGGGATGTAGGGCCACTGAGC AAGAAAGGGTTTTACCTGGCTTTTCAGGATGTGGGTGCTTGCATCGCCCTGGTGTCTGTCCGTGTGTTCT ACAAGAAGTGTCCACTCACCGTTCGAAATCTAGCCCAGTTTCCTGATACCATTACTGGGGCTGATACCTC TTCCCTGGTGGAAGTTCGAGGCTCCTGTGTCAACAACTCAGAAGAGAAGGATGTGCCAAAAATGTACTGT GGGGCAGATGGTGAATGGCTGGTACCCATTGGCAACTGCCTATGCAACGCTGGGCACGAGGAGCAGAATG GTGAATGCCAAGCTTGCAAAATTGGATATTACAAAGCTCTTTCCACGGACGCCTCCTGTGCCAAGTGTCC ACCGCACAGCTACTCTGTCTGGGAAGGAGCCACGTCCTGCACCTGTGACCGAGGCTTTTTCAGAGCTGAC AACGATGCAGCCTCCATGCCCTGCACCCGCCCACCATCTGCTCCCCTCAACTTGATTTCTAATGTCAATG AGACATCGGTGAACTTGGAATGGAGCAGCCCTCAGAACACAGGTGGCCGTCAGGACATTTCTTACAACGT GGTCTGCAAGAAATGTGGAGCTGGTGATCCCAGCAAGTGCCGGCCCTGTGGAAGTGGAGTCCACTACACA CCACAGCAGAACGGACTCAAGACGACCAGAGTCTCCATCACTGACCTCCTAGCACACACCAATTACACGT TTGAGATCTGGGCAGTGAATGGAGTGTCTAAGTATAACCCTAGCCCGGACCAGTCCGTGTCTGTCACAGT GACAACCAACCAAGCAGCACCATCATCCATTGCTTTGGTCCAGGCTAAAGAAGTTACAAGATATAGCGTA GCTCTGGCTTGGCTGGAACCAGATCGACCAAATGGAGTCATCTTAGAATATGAAGTCAAATATTATGAAA AGGATCAGAATGAACGCAGCTATCGCATAGTCCGGACAGCTGCCAGGAACACAGATATCAAAGGCCTGAA CCCTCTGACTTCCTATGTTTTTCACGTGCGAGCCAGGACCGCTGCTGGCTACGGAGACTTCAGCGAGCCC CTGGAAGTCACTACTAATACAGTGCCTTCCCGCATCATTGGCGATGGAGCCAACTCCACTGTCCTGCTGG TCTCCGTCTCTGGCAGTGTGGTCCTCGTGGTCATTCTCATTGCAGCTTTTGTGATCAGCCGAAGACGGAG TAAGTACAGCAAAGCGAAACAAGAAGCAGATGAAGAGAAACATTTGAATCAAGGTGTTAGAACTTATGTG GATCCCTTTACATACGAAGACCCCAACCAGGCAGTTCGAGAATTTGCCAAAGAAATCGATGCATCCTGCA TTAAAATTGAAAAGGTCATAGGAGTTGGTGAATTTGGAGAGGTCTGCAGTGGGCGTCTCAAAGTGCCCGG CAAGAGAGAGATCTGTGTGGCCATCAAGACTCTGAAAGCTGGCTATACTGACAAGCAGAGGAGAGACTTC CTGAGTGAGGCCAGCATCATGGGACAGTTTGACCACCCAAACATAATCCACCTGGAAGGCGTTGTCACCA AATGTAAACCGGTAATGATCATAACGGAGTACATGGAGAACGGCTCCTTGGATGCTTTCCTCAGGAAGAA TGATGGCCGCTTTACAGTCATTCAGCTGGTGGGCATGCTCCGGGGCATTGGCTCGGGGATGAAGTACTTA TCTGATATGAGCTATGTGCATCGAGACCTGGCTGCCAGGAACATCCTGGTAAACAGCAACTTGGTCTGCA AGGTGTCTGACTTTGGCATGTCCAGGGTGCTGGAGGATGACCCCGAAGCAGCCTACACTACCAGGGGTGG CAAGATTCCTATCCGGTGGACTGCGCCAGAAGCAATTGCGTATCGTAAATTTACCTCAGCCAGTGATGTC TGGAGCTACGGAATCGTTATGTGGGAAGTGATGTCATATGGAGAGAGACCGTACTGGGATATGTCCAATC AAGATGTGATCAAAGCCATCGAGGAAGGGTACCGGCTACCCCCTCCAATGGACTGCCCCATTGCCCTCCA TCAGTTAATGCTGGACTGCTGGCAGAAAGAGAGAAGTGACAGGCCTAAATTTGGGCAGATCGTCAACATG CTGGACAAACTCATCCGCAATCCCAACAGCCTGAAGAGGACAGGGTCGGAGAGTTCCAGACCAAACACAG CCTTGTTAGATCCCAGCTCCCCTGAATTCTCTGCTGTAGTATCAGTGGGCGACTGGCTGCAGGCCATCAA AATGGACCGGTATAAGGACAACTTCACGGCCGCCGGGTACACGACACTAGAAGCCGTGGTTCACATGAGC CAGGACGACCTGGCGAGAATTGGCATCACCGCCATCACGCACCAGAATAAGATTTTGAGCAGCGTCCAGG CGATGCGAACCCAGATGCAGCAGATGCATGGCAGGATGGTTCCTGTCTGAGCCAGTACCGAATAGACTCA AAACTCTCGAATAGTTTACCTCATCCATGCACTTTAATTGAAGAACTGCACTTTTTTTACTTTGTCTTCG CCCTCTGAAATTAAAGGAAAACAAACAAACAAAAAACAAAAACAAAAATATCTGCAGCGTTGCTTGGTGT AAGAGATTGCTGAAACCATGGGGCTTATAATGAATGACCTTGATCATCAGAATTAAACCTGGAGCCAGTA ATTTCTCAGAAGAACTCTGTCCATCGCTGGTGTGTAAAATACATGTACTTATATAAATAAAACTCTGCCT CTGAGTTTCGATGCTGTATTTCCCGTCAGAGGCTTTTCAAGTCACCCTAATCCTTTCTCTGTCTGGAATT TCAAGGGAAGGATTTCATCTGTGGCATTAATAACAGTCATCACTCCTTACCAGAAAGAAGACCTGCTGTG CCCAGGAGCACTGTTAAAGGACTCAATGGTGGCTTTCAGGGCTCGGTTTATACCACAGAAGAAAATCCAG GTGAAAGCCACTTGGCCCATTCATGCTGCCTCTTCAATGGCCAGGACACCTGGTGGATTTTTATGACATA GGGGGGGTTAGAAAGAAAAGAAAATGGATTTTTAAAACATGCGAACTCCCAAGACCTATTAACCCTAACT GGACAAATGAGGTCTCTTTGGGGCTGAGGCTGTGCCATATAAAGTCTTCTTTGAGAATGCTACAAACTTC TCATAACTACCTTATGGATAGTGGGCTGAGACAATCCTGAATGGAGAATTTTCACACACACACACACACA CCCCTTCAAAGAGGCAACTCCCAAATTTACAAGGGAATAGATTCTGGTATCTCAGCCTCACAGCCCTTCC TGTTGATTACATAAGCGCTTGGAAGAAAATAGAACACACCCTCCACCGTTCCTTCTGAATGTTTTTCTTT GGCTTCTCTTCTGCCGGTTTTTGAGGCAAAGACACGACAAAACACCTGCACCTGAACAGCAGAAGAAATG TGTCAAGTCCCCTTCGCCCAGGAAAACGACATCCAGCTTTGGCATCTGTTCTCAGCTGCACTGAGGAATG TGCTGTCTCGAGGCACCAGCTCTGCAGAGCCCTTGCTCCCAAACTCCTTGTGGTTTTATTTATACGTATT TCCATATTTCATTCCTGTACGGCCCCGCTGCATCCGTGTGGCAGCAAGTGACCATATGTTACAGGTTTTA CTATGGACGCCAGGTCAGGCGCCACCACGCAGAACAAAGCCAGTTCCGCAAGCTGCCTCCGATACGCAAT GCTAAAGTGACATTTTACCCAGGGTGTGCCATTGCAGTGATATAAATATATTTTTTTCCTAGACTAAAAT ATGACCTGAATATCTCTTTTGATGTGTGTACATAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGT GAGTGTGTATGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAACCTCATGCTTAGAACTGCCTGTGAA TGTTGTGGAATGCTATACCTGGAGAGTTCTGGTTTATTACCAGTTCAAAGATAAGGAAAGAGTCACATGA TATACAAGACCCGGAGACCATGTCCGTCCCCTTGGGAAGACAGCTTGGAAACGCTACATGTTCTTTATCT GTAAGTGTAATGTGTCTGTCACATTCATCCTAAGAAGCAGTTCTTTGGACAGATGTTTTCACCTTGATGG TGGAATGATTTTTAGATATAAGCCTGACTCGCCGATTTCACAATGGAGAGAGAAGGGGATGGAATCCAAT TTGTGACCCCAGATGGAGTATAAGTCGCCTTTGTTTCCCTGCATAAATGAACTTGTTGAATGCGAACAAA TATATGCAATTCATATACTTTTGGAAGATGAACTTAAGATATGTGTGTCAGCCTTGAGATGATGTGTCTG GAGTAATACTTTGTGTCCCAGTGTCACAGAAAAATACATACATGCCAGTGGCTCTTGAGGTTAATGTAGT TGGGATGAAATGGCCTCTAGCAATTCCAGGTTCCCCAATTACATGTGGAAAGTTCTGGTAATTAATATGC AGCTAACTACTTATGCCCTTGAAAGTATACCAGTCTTCTAGAATCGGAGTTCTGGTGTATGAAGATTCAG TATCGACAGGATATGTATACCAAACTCCAGCCAGCATACTGCCATTTTACTTAACCTGAGAGGCTGCCTT GCTTATTCTGAGCTATGGTCACAGAAACTGTGGCCATTTATATAAGCTATAACATCAAATCAGGGAATTA GAAAAAAAAAAAAACGGGGAAAAGATTCTGAACCATATATTGTTGAATAAGTAAAGAAAATCACCAATAA ATATTTATTGCATTCTGACAGGGTGTATGGCATTGTATTCTCATAACTATGCCAGAGTGACAAAGTTATT TCACCCCTTTTGAGGGGACCTTAATATATTTTTAAAGGGATGTGCCTATGCATTGATGCCTGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6512978 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6512978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13403 same embryo
	EMAGE:13406 same embryo
	EMAGE:13405 same embryo
	EurExpress:euxassay_017515 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source