EMAGE mouse gene expression spatial database

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EMAGE:13406

Ska1 spindle and kinetochore associated complex subunit 1 ( MGI:1913718)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017491. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017491_01	euxassay_017491_02	euxassay_017491_03	euxassay_017491_04

euxassay_017491_05	euxassay_017491_06	euxassay_017491_07	euxassay_017491_08	euxassay_017491_09

euxassay_017491_10	euxassay_017491_11	euxassay_017491_12	euxassay_017491_13	euxassay_017491_14

euxassay_017491_15	euxassay_017491_16	euxassay_017491_17	euxassay_017491_18	euxassay_017491_19

euxassay_017491_20	euxassay_017491_21	euxassay_017491_22	euxassay_017491_23	euxassay_017491_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13406_wholemount_strong.wlz

13406_wholemount_moderate.wlz

13406_wholemount_weak.wlz

13406_wholemount_possible.wlz

13406_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13406_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6799
Entity Detected:	Ska1, spindle and kinetochore associated complex subunit 1 ( MGI:1913718)
Sequence:	sense strand is shown >T6799 TCGACCCACGCGTCCGCCCACGCGTCCGCGCAAGGTCGCTGGCTCCGAAGCCGCCCGAAGAGGCATCGCT TAGCAGGTTCCGGGAGCGGGCGACTCGGAGGGTTGGCCCCCGCCTTCCCCGTTCCAGCCTGCGCAAAATC ACGCTTAAAGGATGGACTCAGAGCTAGAAGACTTATGCTCTTATGTTAATGAGAAGATTGGAAATATTAA GAAAATTCTGTCGATAAGAAATCTTGGCCAGGACCCTGCCTTGAAGACCACCTTAAGTAAAATAGGAGAT GAGATCATTGCAGTAAATGAGCTTCTAAATAAATTTGAATTGGAAATTCAGTATCAAGAACAAACTAACA GTTCACTCAAGGAGCTCTGTGAATCTCTTAGAGAAGAGTGTGAAGATGTGGAACATCTCAAAGAACACGT CCCTCCCCATCTGCCTCAAGTGACGGCGACCCAGAGCTTAGTTCACAAGCCCGAGCCGGATCCTAAAGAG TCAGACAAAGCTGAGGAGCCTGGACTGCCGAAGAAGCCCCCCAGAGAGCAGAGAGTTATCAAGGAAATGC AATTTATAACTATGGATGAATTCAGTGACGTTCCTGCGTACATGAAATCCCGTTTAACATATTGTCAAAT TAATGATATTATTAAGGAAATTAACAAGGCAGTAGTTAGCAAATATAAGATTATGCATCAACCAAAAGCA TCTATGAGCTCTGTGAAGAGAAATCTCTACCAGAGATTTATTAATGAAGAAACGAAGGACACGAAAGGTC ATCACTTCATCGTGGAAGCTGACATAAAGGAATTCACGGCTTTGAAAGTTGACAAGAGGTTTTATGTGAT CATGCACATTTTGCGGCATTGCCACAGACTGTCAGAGGTCCGAGGGGGAGGACTTACCCGATACGTCATC ACCTGAGGCTTTTGAAGACTCGGGACTGATTGGCAGCAGGGTATCGAGGGGGGTCTTGAAATTTCATGTA TGTTTTTAGCTTCTCCATTTCCTGGCCTTGTTTGTTACAGAAGTAAAACTTTGGATAAAGCATATATATT TTCAAAACGTACTGCCTTTAGGAACGTGGGTGAAGCTGGAGCTCACAAGCACAGTAAGCCAGTTAGGAAG ACTGACTCGTGTGGAACCTGTGTGTGTATAGGAGTCAGGGGATCATGAGGCCAGGAGGAGGATCTGGAAA AGTGCGGAGAAAAGAGGAACAGAGAAGGCAGTGGACCGCTGTGATAGAGCAGGAGGAAAGACCGTCTGGG AACAGCCAGGGGAAGGGTGGCAAGCACTTGGGCAGGAGGTGGATCAGGATAAAGTGCGATGCCACAAGAT ATAAAAATGCAGTAACAGGACTCATTTCTTTGTGTGCTAACATAAAAATTAATTTTAAAAATCCCTGCCT TTCAGCAAATATTCAGGCACTGCCCTGTCAAGCCTCTGCATGAGTTGTATAATGGACAGCGTGTCTAGTT CTGTTAAGTGCAGATACCATGAGCATAGTATGAGGTCGGGAAAGATGGTGGATACCCAGGTCCTCTGTGG CGTAAAGTCTGATTACTGTTTTTCCACGTGCCCATAGAGAACAGCTTGCCTAAAATGCATTCAGTATAAG CTGGAGGCTCCGAAGTACATTGAGATACTGTCACATGTATTACATATGATATGTATTACAGCTTTACGTC TGGTTCCTTGTTCAGGGTTTCGAAAAATCCTTGTAACGTCCTAAGTGGTAAGGGTGCGTCTTGTGTTGAA CTCAGTTCTTGACTGTAGCTTTCAAACGCCTTTGAAACATTTTTCTGATCAGAACATCTTTTGCTCAGAT TCTAGTGAGGTGATTCTTGGGCGCCTTCAAGGCGGGCACTAGTTACCAAAATACTAAGCCATGACTAGGA AGTTGCAGTTTGGAGCCATTCTCCTGCTCCCGGGGAGGGAGACACCCTTGTTGCACATCCGGCCTCAGCA ACTCAGTTACAGGGGGAGATTTCTTGTCTCGAAAGCCAAAACCACTTGGTCAAAGCTGTCAAGTTCTGCT GCCTGATGAGGCTAGAGATGTTTGGTCATGCCGACAGGCTTCGTCCTTTTTAAAATTAATTTAAAAAAAT CACTGCTCTGGGCCTCCATGCAGGGACACCCCTGTCACACATCTGGCCTCAGCAACTTAGTTGCAGAGAG AGAGATTTCTTGACTCTAAAGCCAGAACCACATGGCTAAAGCTGCCAAGTTCTGTTGCTTTCTGGGGCTG GAGTATGGCCCTGTTGTTCAGTTAGATCTTCACCAGCGGTCTGGTTTTGATAGTTTCCTTCACTGCCTAA GCATGGCTGTCTTGGAACTTGCTCTGTAGACCAGGCTGCTCTTGAACTCAGAGATCTGCATACCCCTGTC TTCTAAGTGCTGGAACTAAAGGCGTGTACCACCCACCACACCTGGACCTAAACTTTCCCTTAATTCCTTT TCATTTTACATCGTTGTAAGCATGGCCACTGTGCCTCACGATATGGATTAAAGGTTCGTGTTCATCCACA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709649 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709649 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13403 same embryo
	EMAGE:13405 same embryo
	EMAGE:13404 same embryo
	EurExpress:euxassay_017491 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source