EMAGE mouse gene expression spatial database

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EMAGE:13539

4933433P14Rik RIKEN cDNA 4933433P14 gene ( MGI:1914037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017642. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017642_01	euxassay_017642_02	euxassay_017642_03	euxassay_017642_04

euxassay_017642_05	euxassay_017642_06	euxassay_017642_07	euxassay_017642_08	euxassay_017642_09

euxassay_017642_10	euxassay_017642_11	euxassay_017642_12	euxassay_017642_13	euxassay_017642_14

euxassay_017642_15	euxassay_017642_16	euxassay_017642_17	euxassay_017642_18	euxassay_017642_19

euxassay_017642_20	euxassay_017642_21	euxassay_017642_22	euxassay_017642_23	euxassay_017642_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13539_wholemount_strong.wlz

13539_wholemount_moderate.wlz

13539_wholemount_weak.wlz

13539_wholemount_possible.wlz

13539_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13539_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T513
Entity Detected:	4933433P14Rik, RIKEN cDNA 4933433P14 gene ( MGI:1914037)
Sequence:	sense strand is shown >T513 TCCTCGAGNCTGTTGGCCTACTGGAGTCTCCGGACGAGGAAGGATAAGAACATGGGCGCTCGCAGAATGG AAACAGACTATAATCCCGTGGAGCTAAGCAGTATGTCTGGATTTGAAGAAGGCTCCGAGCTCAATGGGTT TGAAGGAGCGGATATGAAGGACATGCAGCTGGAGGCCGAGGCAGTTGTAAACGATGTACTCTTTGCTGTC AACCACATGTTTGTCTCAAAAAGCATGCCCTGTGCAGATGATGTGGCTTACATCAATGTGGAGACAAAGG AAAGGAACAGATACTGCCTGGAGCTCACAGAAGCAGGGCTCAGGGTGGTGGGCTATGCTTTTGACCAGGT GGAGGATCATTTGCAAACCCCCTACCATGAGACAGTCTACTCCTTGTTGGATACTCTCAGCCCTGCCTAC CGGGAAGCATTCGGAAACGCGCTCCTTCAGAGACTGGAAGCTTTGAAACGAGATGGACAGTCATGACTCA TGACTCATCTTCCTCCTAATGGGGCCGCTGCTGGTATAGAATCTAACATAAGG
Notes:	The probe template was PCR amplified from IMAGE:1482662 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1482662 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13536 same embryo
	EMAGE:13538 same embryo
	EMAGE:13541 same embryo
	EMAGE:13537 same embryo
	EMAGE:13540 same embryo
	EurExpress:euxassay_017642 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source