EMAGE mouse gene expression spatial database

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EMAGE:13540

Wtap Wilms' tumour 1-associating protein ( MGI:1926395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017678. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017678_01	euxassay_017678_02	euxassay_017678_03	euxassay_017678_04

euxassay_017678_05	euxassay_017678_06	euxassay_017678_07	euxassay_017678_08	euxassay_017678_09

euxassay_017678_10	euxassay_017678_11	euxassay_017678_12	euxassay_017678_13	euxassay_017678_14

euxassay_017678_15	euxassay_017678_16	euxassay_017678_17	euxassay_017678_18	euxassay_017678_19

euxassay_017678_20	euxassay_017678_21	euxassay_017678_22	euxassay_017678_23	euxassay_017678_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13540_wholemount_strong.wlz

13540_wholemount_moderate.wlz

13540_wholemount_weak.wlz

13540_wholemount_possible.wlz

13540_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13540_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4709
Entity Detected:	Wtap, Wilms' tumour 1-associating protein ( MGI:1926395)
Sequence:	sense strand is shown >T4709 CGCAAATAAAGGTTCTCGCCAGCGCTGCGGCGGGAGGAGCGCGGGGGGGCCGGCGGCGGTGCGGCCTCGG CCTGTGTGACTGGCGGCGCCCGCGCAGCCTCGGGCTGGGAAGGGTGCAAGATGACCAACGAAGAACCTCT TCCTAAAAAGGTCCGACTGAGTGAAACAGACTTCAAAGTTATGGCACGGGATGAGTTAATTCTAAGATGG AAACAATATGAAGCATATGTTCAAGCTTTGGAGGGAAAGTACACAGATCTTAATTCAAACGATGTGACTG GTTTAAGGGAATCTGAAGAAAAACTAAAGCAGCAACAGCAGGAGTCTGCACGCAGGGAGAACATTCTTGT CATGCGGCTAGCAACCAAGGAGCAGGAGATGCAAGAGTGCACTACTCAAATCCAGTACCTCAAGCAAGTT CAGCAGCCGAGTGTGGCCCAACTGAGATCAACAATGGTAGACCCAGCGATCAACTTGTTTTTCCTAAAAA TGAAAGGTGAACTGGAACAGACTAAAGACAAACTGGAACAAGCCCAAAATGAACTGAGTGCCTGGAAGTT TACGCCTGATAGGTAAACAAATCATACTCCCCAGTCAAGACTTCCCTGACAGTCCCACCTCGAGAAAGCT GTGGTGGGACAGCCAAGTACTCGTTTCCACACCAAGACTCAGACTTTTTGAGCCAAAAAAGCCACATTCT TATACTGTCCAGCTTGTAATGGTTAATGTAAAACTTAGCAGATGAACCTTGTGTTTCAGCTTTTCTCTTC CCCTCCCCCTTGCTTCAGAGGCCTGATGCCGTCGGACTATTCCGAAGAAGAGGCCAACTCCGAAAAATTC CCCTTCTAGGACATGTAGACACTTTGAGAAGTGTTTCTGTTTGAAGAAAATAGAGGGAAAAACAGAAGTC TTAAGTCTGTGGCACACTGTGTCTTCAGACAGTTTCGAGGATTGAAATCCTAGAGGTTTTAAATCATGAA TTGAACATGTAAAATTCCAGTAAAATGTAAAAATGGAATATGCATCGCTCTTAGCCATGAGCATAGTGAC TTAGAGACACTGTACATCAGTTATGCCAATAAGACTGTGGACTTCATGATTGTTGAACTACTGGGTCAAA ACTCAAATGAGGTGAATTTGCCTTTAAAGGACTTACTTGCTAAGAACCAACTAATAGCCGTGAGACAATC ACGTCATAGCTACCAGTACAAGTAGAGCAAATATTTATCCATTTAGCTCTGAGCTCTATATTATATAATG GAGCCTTAAATCTATGTGGTTTTTATCAATGGTTTGTCTTTTGAATGGTTGTGGAAACTGTAGATAACCT TAACCAAGGACTGTACAAACGTGAAGGTGTGGTCTCACACTTCAGGTTTAAAGTGTTTGAAGCATTATAA GCATTCATTTCACAACTAGATTGTATATGGTATTTGGCTGTGATGAGACTCCCATTATTCAGTAAACCCT CATCTCATTCAGCAGGCACATGTTAAACAGCATTGTCGTTGGTGTGAAATGGGGGAATGTGTTCCTTCAT TGTATTGGGCCTTTTGTATCTATCGAATTCCTGATATTAAATTAAATGTGCCTTGAAATAGTTGGGTTTT TTTTAATTCAAGGAATACTCTGATAATTTTGTTGTATTCAATATTTTACTTTGGGGGGCTATGGAGAGCA GATCAACACTTTGGAAAATTTATTCTGTGCTCTTCAAAGTGAAGGAAAAGGCTGTCTCGCTTAAAAGACT ATTCCAGATGGTATAAAAGACAAGGAGACTTGGCATTTGGCCCCAGGATATTACAGTAGAACATATTGTG GGACAGTCTCACAAGGTGCCTGCCAGAAGATTGGGGAAATAATGTGTTGGATCTCTGGGCTGGCCCAAGT GAGATGGTTTGTGAATTGAAAAAGGTATAGTGATCATTTCTAGCAAACTCATTTGCTCATTTAGATAAAT GAGGCATTATATTCTTCATGCTTCCATCCAATCCTGGAGGTGAATTTTTTTCGTCTATAAGTCACCAGTT TGAATTTTCCCTTGGTGATAATGGAGTCTGAGATGACTAGAAGATAAATGTTCCAGCATGCCCCAACGTT TAAGTGCAGTTTTAACAAGGGTGACTTAAGTTCTCTCTTAATCGTTCCAATCAATTGTAAGTATGCTTAT GTTTGTGCTTTAGTGGACTGACTGTGGGCAGGGTGGGCTGGAGGAATGATGTGTATAATTTTTCAGGAAC AAGTCTGCTTCTAAAATTGTCACTGGGGGTTTGGAGGGTAAGTAAACCAGTCTGTTTAAAAGTAGTTTAT TGTGACAAAATTGGATCTTTTGTATTTATTTCATGCATTTTCAGGTTCATGGTTGAAATGATTCTGAATA TAGTTTTAGTGGGTTGAATAGTAATTGGTAAAATGTATTGCTAGTATGTACTCAGATTTAGCCAGTCTTT CTCTGCTGCTTGAATACAGTGACTTTGAGGCCTGTGGGCCTACTTTCTTGTTATTGTTAATCTTCTCTCT TAATAAGACAATACATGTTCATGTATACAAATGAAATACTAGAGAAAGATGGGAGATGGAAAGTGTCACT CATGGACCTGATGTCAAACTACCCCATTCTGCTCTACAGGACCAGTACCACTGTTAAATATCTTGGGTGT CATCGAGATAATTTGTGGATACACAATGATTTCATGTATATTGGTTTTAAAGAAATGTAATCATATGGTA CATACACATGCTTCAAAGCTTTATTATTTCCCTTCATTTTTATCTTCTAGGTATTTTTTTAGCATATCTA GATTACTACCTTTTTAATGGTCTCTTAGTATTTTTTAATCTTATTTTAACAACCCATCAATGAGGTTTCA TTATATAAAACTAAACCATGTGATATAATGAATCAAGTACTAAAACTACATTATTAATTTGAAAATAAGT AGTACTTAATTAGTAATGTGGAATGATTCCAATGATTGGGTTGTAAGGTTTCCTATATGTATATGGTATT CCTGCCCTTTGCTGCAGGTCTAATTTTGAGGGATAAAGATCTTTTTAGCTGGATGTGGTGGCTTATGCCT TTAATCCCAGCAGTGATGAGGCTGATACAGAAGGAGAGGGGACTTAGGAGTACGTTCATAGATTTGTATC TCAAAGAATAAACAAAAGAACTACTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5035075 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5035075 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13539 same embryo
	EMAGE:13536 same embryo
	EMAGE:13538 same embryo
	EMAGE:13541 same embryo
	EMAGE:13537 same embryo
	EurExpress:euxassay_017678 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source