EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13677

mmu-miR-181b (mmu-miR-181b)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019219. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019219_01	euxassay_019219_02	euxassay_019219_03	euxassay_019219_04

euxassay_019219_05	euxassay_019219_06	euxassay_019219_07	euxassay_019219_08	euxassay_019219_09

euxassay_019219_10	euxassay_019219_11	euxassay_019219_12	euxassay_019219_13	euxassay_019219_14

euxassay_019219_15	euxassay_019219_16	euxassay_019219_17	euxassay_019219_18	euxassay_019219_19

euxassay_019219_20	euxassay_019219_21	euxassay_019219_22	euxassay_019219_23	euxassay_019219_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13677_wholemount_strong.wlz

13677_wholemount_moderate.wlz

13677_wholemount_weak.wlz

13677_wholemount_possible.wlz

13677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 17 19 21 22 23 24

lower lip

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 19 weak expression: see section 10

upper lip

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 22 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70292
Entity Detected:	mmu-miR-181b, (mmu-miR-181b)
Sequence:	sense strand is shown >T70292 AACATTCAACGCTGGTGAGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-181b was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13678 same embryo
	EMAGE:13674 same embryo
	EMAGE:13675 same embryo
	EMAGE:13676 same embryo
	EurExpress:euxassay_019219 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source