Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14109

Klk1b9 kallikrein 1-related peptidase b9 ( MGI:95293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14109 EMAGE:14109 EMAGE:14109 EMAGE:14109
euxassay_003976_01 euxassay_003976_02 euxassay_003976_03 euxassay_003976_04

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14109Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14109_wholemount_strong.wlz
14109_wholemount_moderate.wlz
14109_wholemount_weak.wlz
14109_wholemount_possible.wlz
14109_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14109_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4392
Entity Detected:Klk1b9, kallikrein 1-related peptidase b9 ( MGI:95293)
Sequence:sense strand is shown

>T4392
TGGACACCTGTTACCATGAGGTTCCTGATCCTGTTCCTAGCCCTGTCCCTAGGAGGGATTGATGCTGCAC
CTCCTGTCCACTCTCGAATTGTTGGAGGATTTAAATGTGAGAAGAATTCCCAACCCTGGCATGTGGCTGT
GTACCGTTACAACGAATATATATGCGGGGGTGTCCTGTTGGATGCCAACTGGGTTCTCACAGCTGCCCAC
TGCTATTACGAAGAGAACAAGGTTTCCCTAGGAAAAAACAACCTATACGAAGAGGAACCCTCTGCTCAGC
ACCGATTGGTCAGCAAAAGCTTCCTTCACCCTGGCTACAACAGGAGCCTCCATAGAAACCACATCCGACA
TCCTGAGTATGACTACAGCAATGACCTGATGCTGCTCCGCCTCAGCAAGCCTGCTGACATCACAGATGTT
GTGAAGCCCATCGCCCTGCCTACTGAGGAGCCCAAGCTGGGGAGCACATGCCTTGCCTCAGGCTGGGGCA
GCACTACACCTTTCAAGTTCCAAAATGCAAAAGATCTCCAGTGTGTGAACCTCAAGCTCCTGCCTAATGA
GGACTGTGGCAAAGCCCACATAGAGAAGGTGACAGATGTCATGCTGTGTGCAGGAGAGACAGATGGAGGC
AAAGACACTTGCAAGGGAGACTCAGGAGGCCCACTGATCTGTGATGGTGTTCTCCAAGGTATCACATCAT
GGGGCTTTACCCCATGTGGTGAACCCAAAAAGCCGGGCGTCTACACCAAACTTATTAAGTTTACCTCCTG
GATCAAAGACACTATGGCAAAAAACCTCTGAGTGATACACTGTCTGCCTTTCTCAATAAAATTCACTATG
CAACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4914236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4914236 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14110 same embryo
 EMAGE:14106 same embryo
 EMAGE:14107 same embryo
 EMAGE:14111 same embryo
 EMAGE:14108 same embryo
 EurExpress:euxassay_003976 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS